广西红树林放线菌 Streptomyces olivaceuss 10127 次级代谢产物及降糖降脂活性研究

doi:10.11978/2023198

Abstract

Abstract:

Mangrove has a special ecological environment and abundant actinomycetes resources, which is one of the sources of natural active substances. To obtain compounds with potent biological activity, this study was conducted on the secondary metabolites and hypoglycemic and lipid lowering activities of an actinomyces, Streptomyces olivaceuss 10127. The strain was derived from the rhizosphere soil of mangrove Acanthus ilicifolius L. in Guangxi. The strains were identified by PCR amplification and 16S rRNA gene sequencing, and the secondary metabolites of this strain were isolated by silica gel column, high performance liquid chromatography (HPLC) and semi-preparative liquid chromatography. The structures of monomer compounds were identified using nuclear magnetic resonance (NMR), mass spectra (MS) and optical rotatory dispersion (ORD). The hypoglycemic and lipid-lowering activity of the compound was tested by the kit. From the secondary metabolites of Streptomyces olivaceuss 10127, 12 compounds were isolated. These include (6-(3-methylbut-2-enyl)-H-indol-3-yl) methanethiol (1), methyl indole-3-acetate (2), methyl 4-hydroxyphenylacetate (3), 2-hydroxybenzamide (4), germicidin B (5), germicidin A (6), isogermicidin A (7), 4′-hydroxy-5, 7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone (8), 2, 3-dihydroxybenzoic acid (9), phenyl acetic acid (10), germicidin N (11), piloquinonea (12). Compounds 8 and 12 were isolated from this actinomyces for the first time. The hypoglycemic and lipid-lowering activity of the isolated compounds were assayed for the first time. Compound 1 showed a good effect on lipid reduction. Compound 7 showed both the lipid lowering effect and the glucose lowering effect, surpassing the efficacy of the positive control group. The actinomyces Streptomyces olivaceuss 10127 has potential research value in producing lead compounds with hypoglycemic and lipid lowering activities.

Key words: Streptomyces olivaceuss, secondary metabolites, hypoglycemic activity, lipid-lowering activity

CLC Number:

Q939.9

XIAO Yang, YAN Jing, YANG Shuyu, ZHANG Tangjie, WENG Jie, LI Qilin, LIU Hongcun, MENG Juan, YANG Lifang. Study on the secondary metabolites and hypoglycemic and lipid lowering activities of actinomyces Streptomyces olivaceuss 10127 from the Guangxi mangrove sediments[J].Journal of Tropical Oceanography, 2024, 43(6): 181-189.

Figures/Tables 5

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References 36

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样品	TG含量/(mmol·L^-1)
样品	正常	模型	高	中	低
化合物1	0.06±0.01^**	0.43±0.01^##	0.13±0.01^**	0.28±0.01^**	0.40±0.01^**
化合物7	0.04±0.00^**	0.43±0.01^##	0.21±0.01^**	0.23±0.01^**	0.26±0.01^**
阳性药	0.05±0.02^**	0.43±0.01^##	-	-	0.26±0.01^**

样品	降脂率/%			EC₅₀±SD/(μmol·L^-1)
样品	高	中	低	EC₅₀±SD/(μmol·L^-1)
化合物1	68.93±1.68	36.41±2.06	6.80±1.68	68.02±1.83
化合物7	49.50±1.71	46.53±1.71	39.60±2.97	90.22±15.27
阳性药	-	-	39.53±2.32	46.88±2.23

组别	葡萄糖消耗量/(mmol·L^-1)
正常	1.34±0.15^**
模型	1.87±0.09^##
阳性	2.19±0.02^**
化合物7	2.25±0.04^**

Study on the secondary metabolites and hypoglycemic and lipid lowering activities of actinomyces Streptomyces olivaceuss 10127 from the Guangxi mangrove sediments

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