华南沿海潟湖反硝化微生物活性与群落特征分析

doi:10.11978/2025031

Abstract

Abstract:

The objective of this study was to investigate the activity of denitrifying microorganisms and the factors influencing their community composition in the coastal lagoons of South China, with the aim of elucidating key mechanisms governing the nitrogen cycle in lagoon ecosystems. Three representative lagoons in South China were selected for this research. By measuring the physicochemical parameters of water and sediment, combined with denitrification rates and metagenomic sequencing data, we analyzed the community composition of three denitrifying functional genes (nirK, nirS, nosZ) and their correlations with environmental factors. The results indicated that the denitrification rate in South China lagoons was significantly lower than marine sediment averages, primarily due to low nutrient concentration, high water transparency, and limited water exchange capacity. Notably, the abundance and diversity of nirK genes within the three denitrification functional gene communities were significantly lower than those of nirS and nosZ, suggesting that nirK was more susceptible to environmental variations. Significant differences were observed in the community composition of nirK, nirS and nosZ among different lagoons. At the order level, the dominant species were Nitrosopumilales, Oceanospirillales and Flavobacteriales, respectively, with the main community composition closely resembling that of denitrifying microorganisms in the marine environment. Water transparency, pH and $\text{NH}_{4}^{+}$ concentration were identified as the key environmental factors regulating the composition of the three denitrification functional gene communities. Specifically, the nirS community was more influenced by sediment physicochemical parameters, while the nirK community was more affected by water physicochemical parameters. Additionally, the nirS and nosZ communities were similarly impacted by the same environmental factors, indicating a stronger synergistic effect in the denitrification process. This study provides valuable data to enhance our understanding of denitrification functions and microbial communities in lagoon ecosystems, thereby laying a foundation for their protection and management.

Key words: lagoon, denitrification, community characteristics, gene abundance, nutrient concentration

CLC Number:

P744.2

RONG Qing, XIAN Hanbiao, DONG Xuhui, YE Fei, WU Jiapeng, WANG Yu, LI Jiahui, HONG Yiguo. Denitrifying microbial activity and community characteristics in coastal lagoons of South China[J].Journal of Tropical Oceanography, 2025, 44(6): 108-119.

Figures/Tables 7

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References 39

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潟湖	理化参数
潟湖	S	SD	T	pH	W-$\text{NO}_{3}^{-}$	W-$\text{NH}_{4}^{+}$	W-DIN	W-TN	S-$\text{NH}_{4}^{+}$	S-$\text{NO}_{2}^{-}$	S-DIN	S-TP
DS	-1.000**	/	1.000**	-1.000**	-0.767*	/	0.843**	-0.949**	0.369	0.847**	0.369	-0.525
JH	-0.500	-1.000**	1.000**	-1.000**	-0.685*	0.738*	0.738*	-0.791*	0.474	-0.791*	0.474	-0.482
NSH	-1.000**	-1.000**	-0.429	-1.000**	0.201	-0.891*	0.497	0.891*	-0.754*	-0.088	-0.715*	0.717*
所有潟湖	-0.369	-0.370	0.158	-0.527**	0.407*	0.069	0.350	-0.476*	-0.070	-0.166	-0.035	0.082

Denitrifying microbial activity and community characteristics in coastal lagoons of South China

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