高通量测序分析涠洲油气田开发区的微生物群落结构

doi:10.11978/2016003

热带海洋学报 ›› 2016, Vol. 35 ›› Issue (5): 48-54.doi: 10.11978/2016003CSTR: 32234.14.2016003

高通量测序分析涠洲油气田开发区的微生物群落结构

吴鹏, 连忠廉, 姜重臣, 邓伟, 熊小飞, 时小军, 张敬怀, 娄全胜, 方宏达

国家海洋局南海环境监测中心, 广东广州 510300

收稿日期:2016-01-05 出版日期:2016-08-29 发布日期:2016-09-22
通讯作者: 方宏达, 教授级高级工程师。E-mail: fanghongda1@21cn.com
作者简介:吴鹏(1985—), 男, 江西省吉安市人, 博士, 主要从事海洋环境监测。E-mail: wupeng980521@163.com
基金资助:
基金项目：国家自然科学基金项目(41406179); 广东省自然科学基金项目(2014A030310495); 热带海洋环境国家重点实验室(中国科学院南海海洋研究所)开放课题(LTO1408); 国家海洋局近岸海域生态环境重点实验室资助项目(201508、201608); 国家海洋局南海分局海洋科学技术局长基金资助项目(1516、1511、1233)

Composition of bacterial community in Weizhou oil exploring field revealed by high-throughput sequencing

WU Peng, LIAN Zhonglian, JIANG Zhongchen, DENG Wei, XIONG Xiaofei, SHI Xiaojun, ZHANG Jinghuai, LOU Quansheng, FANG Hongda

South China Sea Environmental Monitoring Center, State Oceanic Administration, Guangzhou 510301, China

Received:2016-01-05 Online:2016-08-29 Published:2016-09-22
Contact: Corresponding author: FANG Hongda, E-mail: fanghongda1@21cn.com
Supported by:
Foundation item: National Natural Science Foundation of China (41406179); Guangdong Natural Science Foundation (2014A030310495); State Key Laboratory of Tropical Oceanography (South China Sea Institute of Oceanology, Chinese Academy of Sciences) (LTO1408); Key Laboratory for Ecological Environment in Coastal Areas, SOA (201508, 201608); Director Fund of Marine Science and Technology, South China Sea Branch, SOA (1516,1511, 1233)

摘要/Abstract

摘要： 基于16S rRNA基因, 采用Illumina MiSeq高通量测序技术分析和比较了涠洲油气田开发区表层海水和沉积物中微生物群落结构。Shannon多样性指数表明涠洲油气田微生物多样性较高, 并且表层海水的微生物多样性要高于沉积物。表层海水中微生物以芽孢杆菌Bacilli居多, 其次为蓝细菌Cyanobacteria、α变形菌Alphaproteobacteria和γ变形菌Gammaproteobacteria; 而沉积物中微生物群落主要类群为γ变形菌。维恩图表明涠洲油气田表层海水的微生物组成或沉积物的微生物组成各自具有一定的相似性, 热图和主成分分析则发现该两类环境介质之间的微生物结构差异较大。Illumina MiSeq高通量测序还发现涠洲油气田存在潜在石油类降解菌假单胞菌属Pseudomonas、鞘氨醇单胞菌属Sphingomonas、红球菌属Rhodococcus和分枝杆菌属Mycobacterium, 并且沉积物中该四类微生物相对比例要高于表层海水, 这一现象可能由环境中石油类含量决定。研究将为评价油气开发活动对微生物群落的影响提供依据, 并为海洋环境中石油污染的生物修复提供基础数据。

关键词: 油气田开发区, 微生物群落, 微生物多样性, 高通量测序, 表层海水, 沉积物

Abstract: The bacterial compositions of surface seawater and sediment in Wei-zhou oil exploring field were investigated and compared by Illumina MiSeq high-throughput sequencing based on 16S rRNA gene in this study. Shannon diversity index values indicated that the bacterial community in Wei-zhou oil exploring field was highly diverse, and the bacterial diversity in the surface seawater samples was much higher than that in the sediment samples. The main bacterial group in the surface seawater samples was Bacilli, followed by Cyanobacteria, Alphaproteobacteria and Gammaproteobacteria, whereas Gammaproteobacteria dominated the sediment samples. Venn diagram demonstrated bacterial composition from the surface seawater and that from the sediment in Wei-zhou oil exploring field displayed high similarity. However, heat map and principal component analysis showed that the samples from seawater and those from the sediment clustered separately. Illumina MiSeq high-throughput sequencing also indicated that some potential oil-degradation bacteria, such as bacteria in genera of Pseudomonas, Sphingomonas, Rhodococcus, and Mycobacterium existed in this field. Furthermore, those four kinds of bacteria in the sediment were more abundant than in the surface seawater, suggesting that the oil concentration in the environment might play an important role in regulating oil-degradation bacterial community. This work provides some new insights toward the effects of oil exploring activities on bacterial community and oil biodegradation potential in marine environment.

Key words: oil exploring field, bacterial community, bacterial diversity, high-throughput sequencing, surface seawater, sediment

吴鹏, 连忠廉, 姜重臣, 邓伟, 熊小飞, 时小军, 张敬怀, 娄全胜, 方宏达. 高通量测序分析涠洲油气田开发区的微生物群落结构[J]. 热带海洋学报, 2016, 35(5): 48-54.

WU Peng, LIAN Zhonglian, JIANG Zhongchen, DENG Wei, XIONG Xiaofei, SHI Xiaojun, ZHANG Jinghuai, LOU Quansheng, FANG Hongda. Composition of bacterial community in Weizhou oil exploring field revealed by high-throughput sequencing[J]. Journal of Tropical Oceanography, 2016, 35(5): 48-54.

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高通量测序分析涠洲油气田开发区的微生物群落结构

Composition of bacterial community in Weizhou oil exploring field revealed by high-throughput sequencing

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