三亚湾珊瑚来源虫黄藻不同株系微环境中微生物群落结构的差异比较分析

doi:10.11978/2022013

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (6): 90-104.doi: 10.11978/2022013CSTR: 32234.14.2022013

三亚湾珊瑚来源虫黄藻不同株系微环境中微生物群落结构的差异比较分析

黄思军¹^,²(), 邱晨¹^,³, 龙超¹^,², 龙丽娟¹^,²^,⁴

1.中国科学院热带海洋生物资源与生态重点实验室, 中国科学院南海海洋研究所, 广东广州 510301
2.南方海洋科学与工程广东省实验室(广州), 广东广州 511458
3.中国科学院大学, 北京 100049
4.三亚海洋生态环境工程研究院, 海南三亚 572000

收稿日期:2022-01-23 修回日期:2022-03-29 出版日期:2022-11-10 发布日期:2022-04-12
通讯作者: 黄思军
作者简介:黄思军(1982—), 男, 福建省光泽县人, 项目研究员, 从事海洋微生物生态学研究。email: huangsijun@scsio.ac.cn
基金资助:
国家自然科学基金项目(41576126);国家自然科学基金项目(42176116);广东省自然科学基金(2017A030306020);中国科学院南海海洋研究所“南海新星”项目(NHXX2019ST0101);中国科学院青年创新促进会会员(2018377)

Phycosphere microbial communities of zooxanthellae cultures isolated from corals in Sanya Bay, South China Sea

HUANG Sijun¹^,²(), QIU Chen¹^,³, LONG Chao¹^,², LONG Lijuan¹^,²^,⁴

1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
3. University of Chinese Academy of Sciences, Beijing 100049, China
4. Sanya Institute of Ocean Eco-Environmental Engineering, Yazhou Scientific Bay, Sanya 572000, China

Received:2022-01-23 Revised:2022-03-29 Online:2022-11-10 Published:2022-04-12
Contact: HUANG Sijun
Supported by:
National Natural Science Foundation of China(41576126);National Natural Science Foundation of China(42176116);Natural Science Foundation of Guangdong Province(2017A030306020);Rising Star Foundation of the South China Sea Institute of Oceanology(NHXX2019ST0101);Youth Innovation Promotion Association CAS(2018377)

摘要/Abstract

摘要：

虫黄藻、细菌和造礁石珊瑚有着密切的共生关系, 但虫黄藻藻际细菌群落尚未得到广泛研究。本研究对5个属的6株虫黄藻进行了离体培养, 其中2株为悬浮型虫黄藻(E型), 4株为贴壁型虫黄藻(A—D型)。通过采集藻株培养物3种粒径的样本开展细菌群落分析, 分别为0.2~3μm(自由生活)、>3μm(附着于藻体或颗粒物)与Settling(沉底贴壁藻体上)。结果发现, 2种生活方式的藻株藻际细菌群落具有显著差别, 贴壁型藻株细菌群落的物种丰富度显著高于悬浮型藻株。发现7个属的细菌广泛存在于所有的藻株中, 它们代表了A—E型虫黄藻藻际细菌的核心类群。对3种粒径样本的核心细菌群落比较发现, 自由生和颗粒附着生的核心细菌群落十分相似, 但均与沉底贴壁样本差异明显。

关键词: 虫黄藻, 珊瑚, 共生关系, 藻际细菌群落, 藻株

Abstract:

Zooxanthellae, bacteria, and reef—building corals have a close symbiotic relationship, but the phycosphere bacterial community of zooxanthellae is poorly studied. In this study, six strains of zooxanthellae from five genera were cultured in vitro, among which two were free-living zooxanthellae strains (type E) and four were anchorage-dependent living zooxanthellae strains (type A—D). Bacterial community analysis was performed by collecting three different particle size fractions (0.2-3μm, >3μm, and settling algae cells). Results showed that the phycosphere bacterial communities between the two lifestyle algal strains were significantly different. The bacterial species richness in anchorage-dependent living strains was significantly higher than that in free-living strains. Seven bacterial genera were observed to be present in all the algal strains, representing the core bacterial communities of zooxanthellae. Comparing the core bacterial communities in three particle size samples, we found that the core bacterial communities in >3μm samples and in 0.2-3μm samples are highly similar, but both were significantly different from the “Settling” samples.

Key words: symbiodinium, coral, symbiotic relationship, phycosphere bacterial community, algae strain

中图分类号:

Q938

黄思军, 邱晨, 龙超, 龙丽娟. 三亚湾珊瑚来源虫黄藻不同株系微环境中微生物群落结构的差异比较分析[J]. 热带海洋学报, 2022, 41(6): 90-104.

HUANG Sijun, QIU Chen, LONG Chao, LONG Lijuan. Phycosphere microbial communities of zooxanthellae cultures isolated from corals in Sanya Bay, South China Sea[J]. Journal of Tropical Oceanography, 2022, 41(6): 90-104.

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藻株	宿主拉丁文名(中文名)	ITS2基因型	藻株分类(属级)
SYSC-2-1	Acropora tenuis (柔枝鹿角珊瑚)	C1	Cladocopium (C型)
SYSC-2-8	Acropora tenuis (柔枝鹿角珊瑚)	E101	Effrenium (E型)
SYSC-14-11	Galaxea fascicularis (丛生盔形珊瑚)	E101	Effrenium (E型)
SYSC-17-3	Galaxea fascicularis (丛生盔形珊瑚)	A6	Symbiodinium (A型)
SYSC-24-3	Platygyra verwyi (小叶扁脑珊瑚)	D1	Durusdinium (D型)
SYSC-28-9	Pavona decussate (十字牡丹珊瑚)	B1	Breviolum (B型)

藻株1	藻株2	两藻株共有ASVs数(取交集)/两藻株总ASVs数(取并集)	所占比例/%
24-3	28-9	35/76	46.05
17-3	24-3	33/73	45.21
17-3	28-9	30/71	42.25
2-8	14-11	16/42	38.10
2-1	28-9	23/82	28.05
2-1	24-3	24/86	27.91
17-3	2-1	20/80	25.00
14-11	2-1	14/62	22.58
2-8	24-3	15/71	21.13
14-11	28-9	11/66	16.67
2-8	24-3	13/79	16.46
14-11	17-3	10/62	16.13
14-11	24-3	11/71	15.49
2-8	28-9	10/77	12.99
2-8	17-3	9/73	12.33

三亚湾珊瑚来源虫黄藻不同株系微环境中微生物群落结构的差异比较分析

Phycosphere microbial communities of zooxanthellae cultures isolated from corals in Sanya Bay, South China Sea

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