分子鉴定方法研究大亚湾水体弧菌种类变化

doi:10.11978/j.issn.1009-5470.2010.04.154

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (4): 154-159.doi: 10.11978/j.issn.1009-5470.2010.04.154cstr: 32234.14.j.issn.1009-5470.2010.04.154

• Marine biology • Previous Articles Next Articles

On dynamic change of Vibrio species in the Daya Bay using molecular identification method

JIANG Xiao1,2, REN Chun-hua1, HU Chao-qun1, LUO Peng1, CHEN Chang1, FENG Jing-bin1,2

1. South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100049, China

Received:2009-11-25 Revised:2010-03-01 Online:2010-07-31 Published:2010-07-29
Contact: 胡超群。 E-mail:cqhu@scsio.ac.cn
About author:江晓(1983—), 湖北省黄冈市人, 硕士研究生, 主要从事弧菌检测与分型研究。
Supported by:
中国科学院知识创新工程重要方向项目(KZCX2-YW-Q07-03); 国家“973”课题(2006CB101803)

Abstract

Abstract:

The authors investigate dynamic change regularity of Vibrio species in the Daya Bay using molecular identification method. Brine from different locations of the Daya Bay was cultured, purified and identified. A total of 368 Vibrio isolates were obtained from 224 seawater samples. Using molecular biology method following biochemical identification, they find Vibrio alginolyticus, V. parahaemolyticus, V. harveyi and V. vulnificus, but not V. cholerae, V. mimicus, V. vulnificus and V. hollisae in the brine from the Daya Bay. The results show that V. alginolyticus and V. parahaemolyticus are the dominant species, which occupy 27.99% and 21.74% of the overall Vibrio isolates, respectively.

Key words: Vibrio, molecular identification, species composition, the Daya Bay, seasonal distribution

JIANG Xiao,REN Chun-hua,HU Chao-qun,LUO Peng,CHEN Chang,FENG Jing-bi. On dynamic change of Vibrio species in the Daya Bay using molecular identification method[J].Journal of Tropical Oceanography, 2010, 29(4): 154-159.

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On dynamic change of Vibrio species in the Daya Bay using molecular identification method

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