小球藻EC04产胞内多糖条件优化及抗氧化活性研究

doi:10.11978/2019016

Abstract

Abstract:

Polysaccharides in Chlorella are involved in many important biological activities and physiological functions. To improve the intracellular polysaccharide production of Chlorella, culture conditions were investigated by single factor experiment and orthogonal experiment. The results showed that the maximum polysaccharide accumulation was obtained at MgSO₄·7H₂O 112.5 mg·L ^-1, K₂HPO₄ 60 mg·L ^-1, NaNO₃ 1.2 g·L ^-1, VB₁ 0.5 mg·L ^-1, and VB₁₂ 0.1 μg·L ^-1. Under these conditions, the polysaccharide production and biomass were 271.74 mg·g ^-1 and 48.027×10 ⁶cells·mL ^-1, which increased by 97.49% and 34.91%, respectively. Free radical scavenging rate of the polysaccharide was also studied. The results showed maximum scavenging rates of DPPH radical and hydroxyl radical were 82.32% and 64.27%, respectively.

Key words: Chlorella sp., polysaccharide, nutrient, vitamin, radical

YANG Haiyan , LI Jieqiong , LIU Hongquan , HUANG Xiaoyan , XU Zhongtao , LUO Yuankang , XUAN Jincai , LONG Han . Study on the optimum conditions for Polysaccharide production of Chlorella EC04 and its antioxidant activity analysis[J].Journal of Tropical Oceanography, 2019, 38(6): 98-104.

Figures/Tables 11

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References 20

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贮存液	配制体积/mL	成分	含量/g	工作液体积/mL
1	1000	NaNO₃	30	50
		K₂HPO₄	0.80
		Na₂CO₃	0.40
2	100	Citrate acid	0.30	2
		柠檬酸铁铵	0.30
		EDTA-Na₂	0.05
3	100	CaCl₂·2H₂O	1.80	2
4	100	MgSO₄·7H₂O	3.75	2
5	1000	H₃BO₃	2.86	1
		MnCl₂-4H₂O	1.81
		CuSO₄·5H₂O	0.079
		Na₂MnO₄·2H₂O	0.391
		Co(NO₃)₂·6H₂O	0.04947
		ZnSO₄·7H₂O	0.222

水平	因素
水平	MgSO₄·7H₂O浓度 /(mg·L^-1)	K₂HPO₄浓度 /(mg·L^-1)	NaNO₃浓度/(g·L^-1)
1	75	40	0.8
2	112.5	60	1.2
3	150	80	1.6

实验组	MgSO₄·7H₂O浓度 /(mg·L^-1)	K₂HPO₄浓度 /(mg·L^-1)	NaNO₃浓度 /(g.L^-1)	空列	多糖产率 /(mg·g^-1)	细胞密度 /(×10⁶cells·mL^-1)
1	3	3	1	3	168.03	48.25
2	1	2	3	2	215.15	49.34
3	3	1	3	1	175.76	41.62
4	1	3	2	3	230.12	49.13
5	2	3	3	2	225.01	52.95
6	3	2	2	1	228.21	50.21
7	2	2	1	3	218.10	45.96
8	2	1	2	1	235.52	42.41
9	1	1	1	2	177.61	35.03
K₁	207.627	196.297	187.913
K₂	226.210	220.487	231.283
K₃	190.667	207.720	205.307
极差R₁	35.543	24.190	43.370
k₁	44.500	39.687	43.080
k₂	47.107	48.503	47.250
k₃	46.693	50.110	47.970
极差R₂	2.607	10.423	4.890

方差来源	离差平方和	自由度d_f	均方	F值	P值
MgSO₄·7H₂O	1896.310	2	948.155	76.899	0.013
K₂HPO₄	878.636	2	439.318	35.630	0.027
NaNO₃	2858.272	2	1429.136	115.908	0.009
误差	24.660	2	12.330

方差来源	离差平方和	自由度d_f	均方	F值	P值
MgSO₄·7H₂O	11.776	2	5.888	6.506	0.133
K₂HPO₄	188.961	2	94.480	104.394	0.009
NaNO₃	41.819	2	20.910	23.104	0.041
误差	1.810	2	0.905

Study on the optimum conditions for Polysaccharide production of Chlorella EC04 and its antioxidant activity analysis

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