Fig. 1

Light microscope (LM) images of strains SYSC-14-11. a) Immobile globular cells and moving gourd-like cells, including binary dividing cells; b) dorsal view and side view, with pyrenoid and nucleus; and c) ventral view, with pyrenoid and nucleus. N: nucleus; PY: pyrenoid "

Fig. 2

Scanning electron microscope (SEM) images of strains SYSC-14-11. a) Apical view showing Elongate Apical Vesicle, five apical plates and x plate; b) apical view showing four apical plates; c) apical-ventral view showing cingulum, cingulum plates, sulcus and sulcus plates; d) dorsal view showing episome, cingulum and hyposome; e) antapical-ventral view showing cingulum and sulcus; and f) antapical view showing hyposome, sulcus plates and posterior sulcal plate"

Fig. 3

Maximum likelihood and neighbor-Joining phylogenetic tree based on LSU sequences with P. beii as outgroup. Bootstrap test (1000 replicates) are shown next to the branches as ML/NJ. For ML, mode/method: Kimura 2-parameter model; Rate among Sites: Gamma distributed with Invariant sites (G + I); No of Discrete Gamma Categories: 5; Gaps/Missing Data Treatment: Partial deletion; Site coverage cutoff: 95%; Select codon positions: 1st + 2nd + 3rd + Noncoding sites; ML Heuristic Method: nearest-neighbor-interchange (NNI). For NJ, mode/method: Kimura 2-parameter model; Substitutions to Include: d Transition + Transversions; Rate among Sites: Gamma Distributed (G); Gamma Parameter: 5, Pattern among Lineages: Same (Homogeneous); Gaps/Missing Data Treatment: Partial deletion; Site coverage cutoff: 95%; Select codon positions: 1st + 2nd + 3rd + Noncoding sites"

Fig. 4

Maximum likelihood and neighbor-Joining phylogenetic tree based on ITS2 region with P. beii as outgroup. Bootstrap test (1000 replicates) are shown next to the branches as ML/NJ. For ML, mode/method: Kimura 2-parameter model; Rate among Sites: Gamma distributed with Invariant sites (G + I); No of Discrete Gamma Categories: 5; Gaps/Missing Data Treatment: Partial deletion; Site coverage cutoff: 95%; Select codon positions: 1st + 2nd + 3rd + Noncoding sites; ML Heuristic Method: nearest-neighbor-interchange (NNI). For NJ, mode/method: Kimura 2-parameter model; Substitutions to Include: d Transition + Transversions; Rate among Sites: Gamma Distributed (G); Gamma Parameter: 5; Pattern among Lineages: Same (Homogeneous); Gaps/Missing Data Treatment: Partial deletion; Site coverage cutoff: 95%; Select codon positions: 1st + 2nd + 3rd + Noncoding sites"