黄鳍棘鲷亲子鉴定技术建立及其在增殖放流效果评估上的应用

doi:10.11978/2024175

Abstract

Abstract:

To evaluate the stock enhancement effectiveness of Acanthopagrus latus, this study employed highly polymorphic microsatellite markers developed by our research group using the species' genome and established a parentage assignment technology. Based on this, we assessed the stock enhancement effectiveness of A. latus in Xiamen Bay sampled in 2023-2024. The specific findings are as follows: (1) Polymorphic primer screening: from 110 randomly selected microsatellite loci in the A. latus genome, we designed primers and preliminarily screened 9 loci with high polymorphism using a wild A. latus population. (2) Establishment of parentage assignment technology: using known pedigree families of A. latus, including 20 parental fish and 72 offspring, we performed parentage analysis with the 9 selected microsatellite loci. As shown by the results, a total of 145 alleles were detected across the loci, with an average allele number (N_a) of 16.11, an average observed heterozygosity (H_o) of 0.788, an average expected heterozygosity (H_e) of 0.810, and an average polymorphic information content (PIC) of 0.785, indicating high polymorphism. The analysis using the Cervus 3.0.7 software showed that under a 95% confidence level, the parentage assignment success rate was 98.61% when the sex of both parents was known, and 97.22% when the sex was unknown. (3) Stock enhancement evaluation: the parentage assignment technology was applied to 114 released parental fish and 264 recaptured individuals. Among the 264 recaptured A. latus, 8 were identified as offspring of the released parents, indicating a resource contribution rate of 3.03% to the wild A. latus population. The results provide reliable technical methods for future genetic resource identification and stock enhancement evaluation of A. latus.

Key words: Acanthopagrus latus, microsatellite, parentage assignment, stock enhancement

CLC Number:

S931.9

LU Youjun, CHEN Qiang, HUANG Liangmin, YOU Songyuan, LIU Dingyu, MA Chao, WU Shuiqing, LIU Xiande. Establishment of parentage assignment techniques in Acanthopagrus latus and its applications in stock enhancement assessment[J].Journal of Tropical Oceanography, 2025, 44(3): 141-147.

Figures/Tables 5

Tab. 1

Tab. 2

Fig. 1

Tab. 3

Tab. 4

References 24

[1]	程家骅, 姜亚洲, 2010. 海洋生物资源增殖放流回顾与展望[J]. 中国水产科学, 17(3): 610-617.
	CHENG JIAHUA, JIANG YAZHOU, 2010. Marine stock enhancement: Review and prospect[J]. Journal of Fishery Sciences of China, 17(3): 610-617 (in Chinese with English abstract).
[2]	成为为, 汪登强, 危起伟, 等, 2014. 基于微卫星标记对长江中上游胭脂鱼增殖放流效果的评估[J]. 中国水产科学, 21(3): 574-580.
	CHENG WEIWEI, WANG DENGQIANG, WEI QIWEI, et al, 2014. Effect of restocking enkancement of Chinese sucker in the middle and upper reaches of Yangtze River based on microsatellite loci[J]. Journal of Fishery Sciences of China, 21(3): 574-580 (in Chinese with English abstract).
[3]	冯晓婷, 杨习文, 杨雪军, 等, 2019. 基于微卫星标记对长江江苏段鳙增殖放流效果评估[J]. 中国水产科学, 26(6): 1185-1193.
	FENG XIAOTING, YANG XIWEN, YANG XUEJUN, et al, 2019. Microsatellite method assessment of the effects of restocking enhancement of bighead carp (Aristichthys nobilis) in Jiangsu reaches of the Yangtse River[J]. Journal of Fishery Sciences of China, 26(6): 1185-1193 (in Chinese with English abstract).
[4]	郭爱环, 原居林, 练青平, 等, 2022. 基于微卫星标记的钱塘江上游黄尾鲴增殖放流效果及其潜在遗传风险评估[J]. 水产学报, 46(12): 2349-2356.
	GUO AIHUAN, YUAN JULIN, LIAN QINGPING, et al, 2022. Stock enhancement effect and potential genetic risks of Xenocypris davidi by molecular markers in the upper reaches of Qiantang River, China[J]. Journal of Fisheries of China, 46(12): 2349-2356 (in Chinese with English abstract).
[5]	江兴龙, 黄永春, 黄良敏, 等, 2013. 厦门湾黄鳍鲷增殖放流效果的评估[J]. 集美大学学报(自然科学版), 18(3): 161-166.
	JIANG XINGLONG, HUANG YONGCHUN, HUANG LIANGMIN, et al, 2013. An evaluation on the effect of Sparus latus enhancement & release in Xiamen Bay[J]. Journal of Jimei University (Natural Science), 18(3): 161-166 (in Chinese with English abstract).
[6]	王九龙, 叶苗, 李洪莉, 等, 2024. 绿鳍马面鲀野生与养殖群体的微卫星遗传多样性分析[J]. 水产科学, 43(2): 312-318.
	WANG JIULONG, YE MIAO, LI HONGLI, et al, 2024. Genetic diversity analysis for wild and cultured filefish Thamnaconus modestus populations revealed by microsatellite markers[J]. Fisheries Science, 43(2): 312-318 (in Chinese with English abstract).
[7]	王新华, 俞小牧, 冯建新, 等, 2016. 黄河鲤全同胞家系的微卫星标记亲子鉴定[J]. 中国水产科学, 23(5): 1023-1031.
	WANG XINHUA, YU XIAOMU, FENG JIANXIN, et al, 2016. Parentage assignment for full-sib families of the Yellow River carp Cyprinus carpio haematoperus based on microsatellites[J]. Journal of Fishery Sciences of China, 23(5): 1023-1031 (in Chinese with English abstract).
[8]	王亚军, 2023. 鲢微卫星标记开发及湖北养殖群体遗传多样性分析[D]. 荆州: 长江大学.
	WANG YAJUN, 2023. Development of microsatellite markers and genetic diversity analysis of cultured Hypophthalmichthys molitrix population in Hubei[D]. Jingzhou: Yangtze University (in Chinese with English abstract).
[9]	杨习文, 刘熠, 薛向平, 等, 2020. 基于微卫星标记的长江江苏段鲢(Hypophthalmichthys molitrix)增殖放流资源贡献率的评估[J]. 湖泊科学, 32(4): 1154-1164.
	YANG XIWEN, LIU YE, XUE XIANGPING, et al, 2020. Resource contribution rate assessment of stock enhancement of silver carp, Hypophthalmichthy smolitrix in Jiangsu section of the Yangtze River based on microsatellite markers[J]. Journal of Lake Sciences, 32(4): 1154-1164 (in Chinese with English abstract).
[10]	余成晨, 沈玉帮, 徐晓雁, 等, 2021. 草鱼生长相关的微卫星标记在选育群体中的验证[J]. 水产学报, 45(3): 321-332.
	YU CHENGCHEN, SHEN YUBANG, XU XIAOYAN, et al, 2021. Verification of microsatellite markers associated with growth traits in selected populations of grass carp (Ctenopharyngodon idella)[J]. Journal of Fisheries of China, 45(3): 321-332 (in Chinese with English abstract).
[11]	张敏莹, 方弟安, 周彦锋, 等, 2022. 基于微卫星标记的钱塘江中下游三角鲂亲本群体和自然捕捞群体遗传多样性及遗传结构[J]. 大连海洋大学学报, 37(5): 775-783.
	ZHANG MINYING, FANG DI’AN, ZHOU YANFENG, et al, 2022. Genetic diversity and genetic structure of four brooder populations and one captive population of black bream Megalobrama terminalisin the middle and lower reaches of Qiantang River by microsatellite marker[J]. Journal of Dalian Ocean University, 37(5): 775-783 (in Chinese with English abstract).
[12]	赵广泰, 刘贤德, 王志勇, 等, 2010. 大黄鱼连续4代选育群体遗传多样性与遗传结构的微卫星分析[J]. 水产学报, 34(4): 500-507.
	ZHAO GUANGTAI, LIU XIANDE, WANG ZHIYONG, et al, 2010. Genetic structure and genetic diversity analysis of four consecutive breeding generations of large yellow croaker (Pseudosciaena crocea) using microsatellite markers[J]. Journal of Fisheries of China, 34(4): 500-507 (in Chinese with English abstract).
[13]	郑卫卫, 陈松林, 李泽宇, 等, 2016. 牙鲆抗迟缓爱德华氏菌性状的遗传力和育种值分析[J]. 农业生物技术学报, 24(8): 1181-1189.
	ZHENG WEIWEI, CHEN SONGLIN, LI ZEYU, et al, 2016. Analyzing of heritability and breeding value of disease resistance for Edwardsiella tarda in Japanese flounder (Paralichthys olivaceus)[J]. Journal of Agricultural Biotechnology, 24(8): 1181-1189 (in Chinese with English abstract).
[14]	朱克诚, 宋岭, 刘宝锁, 等, 2020. 黄鳍棘鲷家系亲缘关系鉴定[J]. 水产学报, 44(3): 351-357.
	ZHU KECHENG, SONG LING, LIU BAOSUO, et al, 2020. Establishment of parentage determination in yellowfin seabream (Acanthopagrus latus)[J]. Journal of Fisheries of China, 44(3): 351-357 (in Chinese with English abstract).
[15]	BOTSTEIN D, WHITE R L, SKOLNICK M, et al, 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. The American Journal of Human Genetics, 32(3): 314-331.
[16]	CASTRO J, PINO A, HERMIDA M, et al, 2007. A microsatellite marker tool for parentage assessment in gilthead seabream (Sparus aurata)[J]. Aquaculture, 272: S210-S216.
[17]	CHEN X, CHO Y, MCCOUCH S, 2002. Sequence divergence of rice microsatellites in Oryza and other plant species[J]. Molecular Genetics and Genomics, 268(3): 331-343.
[18]	JERRY D R, PRESTON N P, CROCOS P J, et al, 2006. Application of DNA parentage analyses for determining relative growth rates of Penaeus japonicus families reared in commercial ponds[J]. Aquaculture, 254(1-4): 171-181.
[19]	JIA PENG, JIN FANMING, XIANG YANGXI, et al, 2022. Establishment and characterization of a fin cell line from yellowfin sea bream (Acanthopagrus latus) and its application to fish virology and toxicology[J]. Aquaculture, 549: 737801.
[20]	NORRIS A T, BRADLEY D G, CUNNINGHAM E P, 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers[J]. Aquaculture, 182(1-2): 73-83.
[21]	PAN CHUANYAN, GAO CHONGMIN, CHEN TAO, et al, 2021. The complete mitochondrial genome of yellowfin seabream, Acanthopagrus latus (Percoiformes, Sparidae) from Beibu Bay[J]. Mitochondrial DNA Part B, 6(4): 1313-1314.
[22]	REN SHENGJIE, MATHER P B, TANG BINGUO, et al, 2022. Standardized microsatellite panels for pedigree management of farmed white-leg shrimp (Penaeus vannamei) stocks validated in a VIE tagged family selection line[J]. Aquaculture, 551: 737946.
[23]	WANG QINGYIN, ZHUANG ZHIMENG, DENG JINGYAO, et al, 2006. Stock enhancement and translocation of the shrimp Penaeus chinensis in China[J]. Fisheries Research, 80(1): 67-79.
[24]	YU YANG, ZHANG XIAOJUN, LIU JINGWEN, et al, 2015. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei[J]. Chinese Journal of Oceanology and Limnology, 33(1): 1-10.

位点	重复单元	荧光标记	引物序列	片段大小	退火温度/℃
AL1	(GT)₁₀	FAM	F: TGTGTCTGTGTGCTCGTGT R: TGGAGCAACAGAAAACACGC	141	57℃
AL2	(GTG)₇	FAM	F: GATCTGGAAATCTGCGGCGA R: TGGCCCGATCACCACTACTA	162	58℃
AL3	(CACG)₅	FAM	F: CTGAATTACACACGTGCACACA R: AGTGGAAGATGAGCACCGTC	140	57.5℃
AL4	(GGAC)₅	HEX	F: GAGGTGATGTGGATGGAGGG R: GCCCCCCCTCTTCTTTCAAA	142	58℃
AL5	(TAG)₇	FAM	F: AAATGTTGGCGTGGTTCACC R: GCAACAGGACACGAAGAAGC	150	57℃
AL6	(TGC)₇	HEX	F: GGTGGGTGGTTAAGTTCAATCC R: AGAATCTTCCAGCCCATTAAGC	195	56℃
AL7	(AGG)₃	FAM	F: GATACTGTCGCAGGCTGAGG R: AACCACACACACACAAAGCC	200	58℃
AL8	(ATC)₃	HEX	F: AGGTAGTACGCAGCACAACC R: CCTTCCAGAGAGTCTCTTTGAC	188	57.5℃
AL9	(AGAT)₄	HEX	F: TACAGTTCTGTGTCGGCTGC R: GATGCTTGAATTTCCTCCGGG	186	58℃

位点	N_a	H_o	H_e	PIC	HWE	F_N
AL1	18	0.787	0.821	0.796	NS	0.0204
AL2	9	0.522	0.747	0.700	**	0.1716
AL3	15	0.815	0.854	0.834	NS	0.0228
AL4	21	0.978	0.906	0.893	ND	-0.0427
AL5	17	0.978	0.852	0.832	*	-0.0779
AL6	11	0.457	0.589	0.557	NS	0.1333
AL7	23	0.780	0.829	0.808	*	0.0068
AL8	10	0.784	0.764	0.725	NS	-0.0197
AL9	21	0.989	0.932	0.922	ND	-0.0331
平均值	16.11	0.788	0.8103	0.785	-	-

位点	N_a	H_o	H_e	PIC	HWE
AL1	29	0.883	0.904	0.895	NS
AL2	11	0.727	0.728	0.682	NS
AL3	24	0.818	0.912	0.904	**
AL4	39	0.864	0.918	0.911	*
AL5	24	0.896	0.882	0.870	***
AL6	40	0.463	0.797	0.786	***
AL7	43	0.939	0.947	0.943	ND
平均值	30	0.798	0.8698	0.8559

子代编号	匹配座位数	体长/mm	体重/g	体高/mm	似然率值	候选亲本编号
HB15	7	140	59.6	52	8.31	A33/A42
HB33	6	150	89.29	56	8.58	A51/A102
HB49	6	148	76.87	59	8.41	A56/A64
HB51	7	148	62.31	50	7.69	A11/A28
HB66	7	155	106.35	64	8.41	A71/A86
HB74	6	145	108.37	65	7.71	A43/A46
HB135	6	132	75.74	57	8.44	A1/A48
HB167	6	177	181.74	79	10.7	A7/A38

Establishment of parentage assignment techniques in Acanthopagrus latus and its applications in stock enhancement assessment

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 24

Related Articles 9

Metrics

Comments

Recommended 0

[1]	WU Guiqing, LI Ruihua, XIAO Yihao, CHEN Yanlin, LUO Xuan, LIU Xiangquan, ZHU Jiajie, WU Xueping. Analysis of genetic diversity in 9 populations of Sinonovacula constricta using microsatellite markers [J]. Journal of Tropical Oceanography, 2025, 44(2): 124-136.
[2]	ZHENG Guobin, ZHAO Hongbo, HUANG Liangmin, ZHANG Jing, LIU Xiande. Discovery and verification of SNP in Acanthopagrus latus [J]. Journal of Tropical Oceanography, 2023, 42(2): 78-86.
[3]	FU Chengchong, LI Fuyu, CHEN Dandan, HOU Jing, WANG Jun, LI Yuanchao, WANG Daoru, WANG Yan. The genetic structure and connectivity of Porites lutea metapopulation of the fringing reefs around the Hainan Island [J]. Journal of Tropical Oceanography, 2023, 42(2): 64-77.
[4]	HONG Xiaofan, CHEN Zuozhi, ZHANG Jun, JIANG Yan’e, GONG Yuyan, CAI Yancong, YANG Yutao. Analysis of ecological carrying capacity of reef organisms in Qilianyu Islands based on Ecopath model [J]. Journal of Tropical Oceanography, 2022, 41(1): 15-27.
[5]	Zhiying ZHAO, Liyun LIANG, Lirong BAI. Analysis of genetic diversity among three wild populations of Penaeus monodon using microsatellite marker [J]. Journal of Tropical Oceanography, 2018, 37(3): 65-72.
[6]	LI Li-hao,YU Da-hui,HUANG Gui-ju,DU Bo,FU Yun,TONG Xin,GUO Yi-hui,YE Wei. Comparison of genetic diversity among stocks of Oreochromis niloticus, O. aureus and red tilapia based on microsatellite DNA [J]. Journal of Tropical Oceanography, 2012, 31(2): 102-109.
[7]	JI Lei,OU You-jun,LI Jia-er. Genetic polymorphism of three cultured populations of golden pompano Trachinotus ovatus as revealed by microsatellites [J]. Journal of Tropical Oceanography, 2011, 30(3): 62-68.
[8]	GUO Yu-song,WANG Zhong-duo,LIU Chu-wu,CHEN Zhi-ming,LIU Yun. Isolation and genetic diversity analysis of microsatellites from nine species of familiar snappers [J]. Journal of Tropical Oceanography, 2010, 29(3): 82-86.
[9]	QU Ni-ni ,GONG Shi-yuan ,HUANG Gui-ju ,TONG Jin-gou ,YU Da-hui . Isolation and screening of microsatellite markers from the Chinese pearl oyster Pinctada fucata based on FIASCO [J]. Journal of Tropical Oceanography, 2010, 29(3): 47-54.