Journal of Tropical Oceanography ›› 2018, Vol. 37 ›› Issue (3): 65-72.doi: 10.11978/2017084CSTR: 32234.14.2017084
• Orginal Article • Previous Articles Next Articles
Zhiying ZHAO1,2(), Liyun LIANG2, Lirong BAI1,2
Received:
2017-07-30
Revised:
2017-10-17
Online:
2018-06-10
Published:
2018-05-03
Supported by:
Zhiying ZHAO, Liyun LIANG, Lirong BAI. Analysis of genetic diversity among three wild populations of Penaeus monodon using microsatellite marker[J].Journal of Tropical Oceanography, 2018, 37(3): 65-72.
Tab. 1
Fourteen pairs of effective microsatellites primers sequence and their annealing temperature"
微卫星位点 | GenBank序列号 | 引物序列(5′-3′) | 退火温度/℃ |
---|---|---|---|
DTLPM218 | AY 189727 | F: ATTCCGCAATATATCGGTTTCC | 57 |
R: AATGTTTCCATTTCATGCTTCG | |||
DTLPM303 | AY 188984 | F: TGCCTTGTATTTTGACGATCAG | 56 |
R: TTGGAGTAGCAACAGCGGTA | |||
DTLPM402 | AY 188987 | F: CCACTCTAACTCCGCCAGTC | 54 |
R: TCCCTACCCCACTATCATCG | |||
DTLPM 120 | AY188978 | F: TTATCCGTATAGCCGCGTTATC | 52 |
R: TTACAGGACCTGCATTTGTGTC | |||
DTLPM 104 | AY188974 | F: AGGACCTGCATTTGTGTCG | 52 |
R: ATGGCGAGACAAGGTTCG | |||
BC52 | AF097631 | F: AAGCAGATAGACCGTCAG | 52 |
R: AATCCTGCAGAGCAATAC | |||
TUZXPM49 | AF077568 | F: ATCTGACAGGGCACCATAC | 54 |
R: AGTCGAGTCTTGAATAAGCG | |||
PM138 | P1-AY500853 | F: ACGGAGTGGGTAGAGACATA | 56 |
R: ACAAGCGAAGTGAAGAGG | |||
PM205 | AY500854 | F: AGGAATGATGGGAGGGAAAG | 56 |
R: AAGCTCAGGCAAGCGTGTAT | |||
PM528 | AY500855 | F: GTGTTATTTTCCACGGGTGC | 56 |
R: GCTGCAGGAAGTGTAGGGAG | |||
PM1713 | AY500858 | F: GTTGCGACGGGTTGATTC | 54 |
R: TTTATGGCTATGGCTGACAC | |||
PM2345 | AY500860 | F: GATATTTCAAGGAATGCTCG | 54 |
R: TAATTCGTGCCTTACCTCAT | |||
PM3852 | AY500862 | F: TAATGGGCGTAAGTCTTCGG | 56 |
R: TGAAAGGAGTCGGGATATGC | |||
PM4793 | AY500867 | F: CTTCTAGCGCCATTTCAAGG | 56 |
R: TCCTTCCAGTGTTCGGAGTT |
Tab. 2
Allelic frequency of 14 microsatellite loci in three populations of P. monodon"
位点 | 等位基因 | 群体 | 位点 | 等位基因 | 群体 | ||||
---|---|---|---|---|---|---|---|---|---|
琼海 | 三亚 | 湛江 | 琼海 | 三亚 | 湛江 | ||||
PM1384 | A | 0.500 | 0.4667 | 0.4167 | PM1383 | A | 0.7333 | 0.2667 | 0.4667 |
B | 0.0667 | 0.0167 | 0.0333 | B | 0.1500 | 0.4000 | 0.2333 | ||
C | 0.400 | 0.3000 | 0.4833 | C | 0.1167 | 0.3333 | 0.3000 | ||
D | 0.0333 | 0.2167 | 0.0667 | ||||||
TUZXPM49 | A | 0.0500 | 0.0333 | 0.0167 | PM1382 | A | 0.3000 | 0.3000 | 0.4667 |
B | 0.5167 | 0.5667 | 0.4667 | B | 0.3000 | 0.0833 | 0.0500 | ||
C | 0.4333 | 0.4000 | 0.5167 | C | 0.4000 | 0.6167 | 0.4833 | ||
PM1713 | A | 0.7333 | 0.6500 | 0.7333 | DTLPM104 | A | 0.2333 | 0.1000 | 0.0500 |
B | 0.2667 | 0.3500 | 0.2667 | B | 0.6833 | 0.7833 | 0.6333 | ||
C | 0.0833 | 0.1167 | 0.3167 | ||||||
BC52 | A | 0.3167 | 0.5833 | 0.0667 | PM1381 | A | 0.6500 | 0.4667 | 0.3833 |
B | 0.6833 | .4167 | 0.9333 | B | 0.3500 | 0.5333 | 0.6167 | ||
PM528 | A | 0.3167 | 0.1500 | 0.3167 | PM138 | A | 0.0500 | — | 0.0667 |
B | 0.5333 | 0.6333 | 0.5167 | B | 0.6833 | 0.9333 | 0.8333 | ||
C | 0.1500 | 0.2167 | 0.1667 | C | 0.2667 | 0.0667 | 0.1000 | ||
PM2345 | A | 0.0833 | 0.0667 | 0.0167 | PM3852 | A | 0.1667 | 0.1833 | 0.1333 |
B | 0.8667 | 0.9333 | 0.9333 | B | 0.5833 | 0.6500 | 0.6333 | ||
C | 0.0500 | — | 0.0500 | C | 0.2500 | 0.1667 | 0.2333 | ||
PM205 | A | 0.6167 | 0.6167 | 0.6667 | PM4793 | A | 0.5167 | 0.4333 | 0.7500 |
B | 0.3833 | 0.3833 | 0.3333 | B | 0.4833 | 0.5667 | 0.2500 |
Tab. 3
Genetic variation of three populations (Qionghai, Sanya, and Zhanjiang) of P. monodon"
位点 | 等位 基因数 | 有效等位 基因数 | 观察杂合度 | 期望杂合度 | HW平衡偏离指数 | HW 平衡检测 | 多态信息含量 |
---|---|---|---|---|---|---|---|
DTLPM120 | 4/4/4 | 2.4064/2.8169/2.4226 | 1.000/0.9333/0.9333 | 0.5844/0.6450/0.5872 | 0.7111/0.4470/0.5894 | **/**/* | 0.5804/0.6352/0.5842 |
TUZXPM49 | 3/3/3 | 2.1871/2.0737/2.0619 | 0.4667/0.6667/0.4000 | 0.5428/0.5178/0.5150 | -0.1402/0.2876/-0.2233 | ns/ns/ns | 0.4212/0.4162/0.3985 |
PM1713 | 2/2/2 | 1.6423/1.8349/1.6423 | 0.2667/0.5667/0.4667 | 0.3911/0.4550/0.3911 | -0.3181/0.2455/0.1933 | ns/ns/ns | 0.3146/0.3515/0.3146 |
BC52 | 2/2/2 | 1.7630/1.9459/1.1421 | 0.5667/0.3667/0.1333 | 0.4328/0.4861/0.1244 | 0.3094/-0.2456/-0.0715 | ns/ns/ns | 0.3391/0.3668/0.1168 |
DTLPM402 | 3/3/3 | 1.7425/2.9221/2.7607 | 0.3333/0.3000/0.4000 | 0.4261/0.6578/0.6378 | -0.2178/-0.5439/-0.3728 | ns/**/* | 0.4013/0.5995/0.6043 |
DTLPM303 | 3/3/3 | 2.9412/2.0955/2.2032 | 0.5000/0.6333/0.4000 | 0.6600/0.5228/0.5461 | -0.2424/0.2114/-0.2675 | ns/ns/ns | 0.51/0.5162/0.5439 |
DTLPM104 | 3/3/3 | 1.8927/1.5693/1.9846 | 0.4667/0.4333/0.4667 | 0.4717/0.3628/0.4961 | -0.011/0.1943/-0.0593 | ns/ns/ns | 0.4146/0.3338/0.4137 |
DTLPM218 | 2/2/2 | 1.8349/1.9912/1.8967 | 0.4333/0.4667/0.2333 | 0.4550/0.4978/0.4728 | -0.0478/-0.0625/-0.5066 | ns/ns/** | 0.3515/0.3739/0.3857 |
PM528 | 3/3/3 | 2.4557/2.1251/2.5316 | 0.4000/0.3000/0.4333 | 0.5928/0.5294/0.6050 | -0.3252/-0.4333/-0.2838 | */**/ns | 0.5231/0.4738/0.5365 |
PM205 | 2/2/2 | 1.8967/1.8967/1.8000 | 0.3667/0.6333/0.6000 | 0.4728/0.4728/0.4444 | -0.2244/0.3395/0.3501 | ns/ns/ns | 0.3610/0.3610/0.3457 |
PM138 | 3/2/3 | 1.8499/1.1421/1.4107 | 0.4333/0.0000/0.1333 | 0.4594/0.1244/0.2911 | -0.0568/-1.000/-0.5421 | ns/**/** | 0.3902/0.1168/0.2711 |
PM3852 | 3/3/3 | 2.3226/2.0666/2.1127 | 0.3667/0.2333/0.2333 | 0.5694/0.5161/0.5267 | -0.3559/-0.5480/-0.5571 | */**/** | 0.5025/0.4642/0.4688 |
PM2345 | 3/2/3 | 1.3148/1.1421/1.1443 | 0.1667/0.0667/0.0667 | 0.2394/0.1244/0.1261 | -0.3037/-0.4638/-0.4711 | **/*/** | 0.2252/0.1168/0.1213 |
PM4793 | 2/2/2 | 1.9978/1.9651/1.6000 | 0.1000/0.5333/0.2333 | 0.4994/0.4911/0.3750 | -0.7998/0.0860/-0.3787 | **/ns/* | 0.3747/0.3705/0.3047 |
平均 | 2.7143/ 2.5714/ 2.7143 | 2.0185/1.9715/1.9081 | 0.4214/0.4357/0.3667 | 0.4857/0.4575/0.4385 | -0.2926/-0.2809/-0.2817 | ns/ns/ns | 0.4078/0.3926/0.3864 |
Tab. 5
F-value and gene flow of 14 microsatellite loci in the three populations of P. monodon"
微卫星位点 | 近交系数 | 遗传分化系数 | 基因流 | 微卫星位点 | 近交系数 | 遗传分化系数 | 基因流 |
---|---|---|---|---|---|---|---|
DTLPM120 | -0.5780 | 0.0219 | 11.1477 | DTLPM402 | 0.4197 | 0.0887 | 2.5679 |
TUZXPM49 | 0.0070 | 0.0079 | 31.3235 | DTLPM218 | 0.2050 | 0.0496 | 4.7873 |
PM1713 | -0.0507 | 0.0074 | 33.4050 | DTLPM303 | 0.1131 | 0.0438 | 5.4533 |
BC52 | -0.0224 | 0.2038 | 0.9768 | DTLPM104 | -0.0271 | 0.0442 | 5.4104 |
PM528 | 0.3438 | 0.0165 | 14.9471 | PM3852 | 0.4831 | 0.0047 | 53.0854 |
PM205 | -0.1511 | 0.0024 | 104.2500 | PM2345 | 0.3878 | 0.0142 | 17.4079 |
PM138 | 0.3524 | 0.0612 | 3.8352 | PM4793 | 0.3653 | 0.0732 | 3.1675 |
平均 | 0.1143 | 0.0456 | 5.2321 |
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