Journal of Tropical Oceanography ›› 2011, Vol. 30 ›› Issue (3): 62-68.doi: 10.11978/j.issn.1009-5470.2011.03.062cstr: 32234.14.j.issn.1009-5470.2011.03.062

• Marine biology • Previous Articles     Next Articles

Genetic polymorphism of three cultured populations of  golden pompano Trachinotus ovatus as revealed by microsatellites

JI Lei1, 2, OU You-jun1, LI Jia-er1   

  1. 1. South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • Received:2010-05-10 Revised:2010-06-29 Online:2011-07-18 Published:2011-07-20
  • Contact: 区又君。E-mail: ouyoujun@126.com

Abstract:

Amplifications with six pairs of microsatellite primers were used to determine the genetic polymorphism of genomic DNA in three cultured populations of golden pompano Trachinotus ovatus, which were collected in Hainan, Shenzheng, and Fujian provinces. The data were analyzed by software package PopGen ver. 32, and their phylogenetic trees were constructed using Mega ver. 4.0. The results show that the value of average number of alleles (Na) was 3.67-3.83 in all three populations; the value of average effective number of alleles (Ne) was 2.43-3.03; the observed value of mean heterozygosity (Ho) varied from 48% to 66%; the expected value of mean heterozygosity (He) varied from 56% to 64%; and the value of average polymorphism information content (PIC) was 0.49-0.55. A high genetic diversity among the three populations could be concluded from the data collected. In addition, we computed the Hardy-Weinberg index to access the distribution of genotypes, and the values in Hainan, Shenzhen, and Fujian populations were 0.10, 0.12, and -0.08, respectively. The Bonferroni Correction test showed that there is no significant genetic disequilibrium among the three populations (p0.05). Cluster analysis was also carried out based on the Nei's genetic identity and genetic distance. The results reveal that phylogenetic relationship between the populations in Fujian and Shenzhen was closer, and the origins of these populations may request further studies.

Key words: Trachinotus ovatus , cultured population , microsatellite DNA , genetic diversity