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热带海洋学报

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基于OSMAC策略的Nocardiopsis alba SCSIO XS060次级代谢产物多样性挖掘及其生物合成途径推测

张馨予1,2, 董平3, 葛雯3, 鞠建华2, 高程海1, 易湘茜1*, 马俊英2*
  

  1. 1. 广西中医药大学海洋药物研究院, 广西 南宁 530200;


    2. 广东省海洋药物重点实验室(中国科学院南海海洋研究所), 广东 广州 510301;


    3. 遵义医科大学, 贵州 遵义 563000


  • 收稿日期:2026-05-31 修回日期:2026-06-12 接受日期:2026-06-20
  • 通讯作者: 马俊英
  • 基金资助:
    国家自然科学基金(U23A20107); 广东省基础与应用基础研究基金(2023B1515120053)

OSMAC Strategy-Driven Diversity Mining of Secondary Metabolites from Nocardiopsis alba SCSIO XS060 and Prediction of Their Biosynthetic Pathway

ZHANG Xinyu1, 2, DONG Ping3, GE Wen3, JU Jianhua2, GAO Chenghai1, YI Xiangqian1*, MA Junying2*    

  1. 1. Institute of Marine Materia Medica, Guangxi University of Chinese Medicine, Nanning 530200, China;


    2. Guangdong Key Laboratory of Marine Materia medica(South China Sea Institute of Oceanology), Guangzhou 510301, China;


    3. Zunyi Medical University, Zunyi 563000, China;


  • Received:2026-05-31 Revised:2026-06-12 Accepted:2026-06-20
  • Supported by:

     National Natural Science Foundation of China (U23A20107); Guangdong Basic and Applid Basic Rescarch Foundation (2023B1515120053)

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摘要： 本文从西沙群岛珊瑚来源的样品中筛选得到一株放线菌, 鉴定其种属后命名为Nocardiopsis alba SCSIO XS060。基于OSMAC(One Strain Many Compounds)策略挖掘其次级代谢产物, 从中分离得到8个天然产物, nocapyrone B(1)、marinactinone A(2)、nocapyrone L(3)、nocapyrone H(4)、(2E/5Z)-2-[(4-methoxyph-enyl)methylene]-5-(2-methylpr-opylidene)-3,6-piperazinedione(5)、malassezione(6)、2-Hydroxy-3-methoxybenz-oic acid(7)、1-hydroxy-4-met-hoxy-2-naphthoic acid(8)。抗菌活性测试结果显示, 化合物5对耐甲氧西林金黄色葡萄球菌MRSA(methicillin-resistant Staphylococcus aureus)和金黄色葡萄球菌(Staphylococcus aureus)有抗菌活性, MIC(minimal inhibitory concentration)值分别为32μg·mL-1、16μg·mL-1, 化合物2对枯草芽孢杆菌 (Bacillus subtilis)有弱抗菌活性。通过生物信息学分析, 确定了cluster 9为nocapyrone生物合成基因簇, 命名为npr基因簇, 基于化学结构和生物合成原理推测了其生物合成途径。本研究基于OSMAC策略从菌株 Nocardiopsis alba SCSIO XS060中分离得到了系列化合物, 为聚酮或吩嗪类等分子的生产提供了新的菌株资源。

关键词: 放线菌, 次级代谢产物, OSMAC, 聚酮, 吩嗪

Abstract: In this study, a coral-derived actinomycete strain was isolated from samples collected from the Xisha Islands and identified as Nocardiopsis alba SCSIO XS060 based on taxonomic characterization. Guided by the OSMAC (One Strain Many Compounds) strategy, the secondary metabolites of this strain were systematically investigated, leading to the isolation of eight natural products: nocapyrone B (1), marinactinone A (2), nocapyrone L (3), nocapyrone H (4), (2E/5Z)-2-[(4-methoxyphenyl)methylene]-5-(2-methylpropylidene)-3,6-piperazinedione (5), malassezione (6), 2-hydroxy-3-methoxybenzoic acid (7), and 1-hydroxy-4-methoxy-2-naphthoic acid (8). Antibacterial assays demonstrated that compound 5 exhibited antibacterial activity against MRSA (methicillin-resistant Staphylococcus aureus) and Staphylococcus aureus, with MIC (minimal inhibitory concentration) values of 32μg·mL-1 and 16μg·mL-1, respectively, while compound 2 showed weak antibacterial activity against Bacillus subtilis. Through bioinformatic analysis, cluster 9 was identified as the biosynthetic gene cluster responsible for nocapyrone production and was designated as the npr biosynthetic gene cluster. Based on the chemical structures and biosynthetic logic, a plausible biosynthetic pathway was proposed. Overall, this study demonstrated that the OSMAC strategy enabled the discovery of a series of secondary metabolites from Nocardiopsis alba SCSIO XS060, providing a new microbial resource for the production of polyketide or phenazine-related molecules.

Key words: actinomycetes, secondary metabolites, OSMAC, polyketides, phenazines