海绵共附生真菌Penicillium crustosum SCSIO 41442活性代谢产物研究

doi:10.11978/2025025

热带海洋学报 ›› 2025, Vol. 44 ›› Issue (6): 188-195.doi: 10.11978/2025025CSTR: 32234.14.2025025

• 海洋资源开发 • 上一篇

海绵共附生真菌Penicillium crustosum SCSIO 41442活性代谢产物研究

梁嘉琦¹^,²(), 陈欣琪¹^,², 陈仪¹^,², 丛梦静¹, 曹沁琳¹, 黄晓雯¹^,³, 刘永宏¹, JAYASINGHE Lalith¹^,⁴, 陈春梅¹(), 周雪峰¹^,²()

¹ 中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东省海洋药物重点实验室, 广东广州 510301
² 中国科学院大学, 北京 100049
³ 桂林医学院, 广西桂林 541199
⁴ 国家基础科学研究所, 斯里兰卡康提 20000

收稿日期:2025-02-18 修回日期:2025-03-19 出版日期:2025-11-10 发布日期:2025-12-03
通讯作者: 陈春梅, email: chenchunmei18@mails.ucas.ac.cn;周雪峰, email: xfzhou@scsio.ac.cn
作者简介:
梁嘉琦(2000—), 女, 河北省张家口市人, 博士研究生, 从事海洋微生物天然产物研究。email: liangjiaqi22@mails.ucas.ac.cn
基金资助:
广东省自然资源厅海洋经济发展专项(GDNRC[2023]37)

Bioactive metabolites from the sponge-derived fungus Penicillium crustosum SCSIO 41442

LIANG Jiaqi¹^,²(), CHEN Xinqi¹^,², CHEN Yi¹^,², CONG Mengjing¹, CAO Qinlin¹, HUANG Xiaowen¹^,³, LIU Yonghong¹, JAYASINGHE Lalith¹^,⁴, CHEN Chunmei¹(), ZHOU Xuefeng¹^,²()

¹ CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
² University of Chinese Academy of Sciences, Beijing 100049, China
³ Pharmacy school of Guilin Medical University, Guilin 541199, China
⁴ National Institute of Fundamental Studies, Kandy 20000, Sri Lanka

Received:2025-02-18 Revised:2025-03-19 Online:2025-11-10 Published:2025-12-03
Contact: CHEN Chunmei, email: chenchunmei18@mails.ucas.ac.cn;ZHOU Xuefeng, email: xfzhou@scsio.ac.cn
Supported by:
Special Project for Marine Economic Development of Department of Natural Resources of Guangdong Province(GDNRC[2023]37)

摘要/Abstract

摘要：

从海绵共附生真菌Penicillium crustosum SCSIO 41442中分离得到1个新生物碱diacedolinate(1)和14个已知化合物(2~15)。通过光谱学分析和电子圆二色谱(ECD)测定了所有化合物的结构, 并对这些化合物进行了抗氧化和抗菌活性测试。结果表明, 化合物1具有弱抗氧化活性, 半抑制浓度(half maximal inhibitory concentration, IC₅₀)为(71.00±0.14)μg·mL^-1。与阳性对照维生素C相比, 化合物2表现出强抗氧化活性, IC₅₀为(1.25±0.10)μg·mL^-1。化合物9、10、11和15对耐甲氧西林葡萄球菌(methicillin-resistant Staphylococcus aureus, MRSA)、Colletotrichum asianum HNM 408、Colletotrichum acutatum HNM RC178 和 Alternaria alternate多种病原菌表现出广谱的抗菌活性, 最小抑菌浓度(minimum inhibitory concentration, MIC)为2.5~160μg·mL^-1。这些化合物的活性均为首次报道。

关键词: 海绵共附生真菌, Penicillium crustosum, 次级代谢产物, 抗氧化, 抗菌

Abstract:

A new alkaloid, diacedolinate (1), along with fourteen known compounds (2-15) was isolated from the sponge associated fungus Penicillium crustosum SCSIO 41442. The structures of these compounds were determined by spectrum analysis and ECD. All compounds were evaluated for their antioxidant and antimicrobial activities. The results showed that compound 1 exhibited weak antioxidant activity with an IC₅₀ value of (71.00±0.14) μg·mL^-1, while compound 2, in contrast, displayed broad antioxidant activity with an IC₅₀ value of (1.25±0.10) μg·mL^-1, compared with the positive control, vitamin C. In addition, compounds 9, 10, 11, and 15 demonstrated broad-spectrum antimicrobial activity against a variety of pathogens, including MRSA, Colletotrichum asianum HNM 408, Colletotrichum acutatum HNM RC178, and Alternaria alternate, with MIC values ranging from 2.5 to 160 μg·mL^-1. The bioactivities of these compounds are reported here for the first time.

Key words: sponge-derived fungi, Penicillium crustosum, secondary metabolites, antioxidant, antimicrobial

中图分类号:

Q936

梁嘉琦, 陈欣琪, 陈仪, 丛梦静, 曹沁琳, 黄晓雯, 刘永宏, JAYASINGHE Lalith, 陈春梅, 周雪峰. 海绵共附生真菌Penicillium crustosum SCSIO 41442活性代谢产物研究[J]. 热带海洋学报, 2025, 44(6): 188-195.

LIANG Jiaqi, CHEN Xinqi, CHEN Yi, CONG Mengjing, CAO Qinlin, HUANG Xiaowen, LIU Yonghong, JAYASINGHE Lalith, CHEN Chunmei, ZHOU Xuefeng. Bioactive metabolites from the sponge-derived fungus Penicillium crustosum SCSIO 41442[J]. Journal of Tropical Oceanography, 2025, 44(6): 188-195.

图/表 6

参考文献 20

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Position	δ_H, mult (J in Hz)	δ_C, type
2		174.75, C
3	4.17, s	75.35, CH
3a		133.42, C
4	6.89, d (2.2)	113.05, CH
5		154.52, C
6	6.67, dd (8.3, 2.2)	116.67, CH
7	6.71, d (8.7)	111.76, CH
7a		135.39, C
8		175.73, C
9	2.56, s	30.07, CH₃

Compound	IC₅₀/( μg·mL^-1)
1	71.00±0.14
2	1.25±0.10
Vitamin C ^a	5.90±0.05

Compound	MRSA	C. acutatum HNM RC178	C. asianum HNM 408	A. alternate
9	>160	2.5	>160	>160
10	>160	>160	5.0	>160
11	2.5	>160	80.0	>160
15	>160	>160	>160	160.0
Ampicillin ^a	0.625	-	-	-
Nystatin ^b	-	0.625	2.5	5.0

海绵共附生真菌Penicillium crustosum SCSIO 41442活性代谢产物研究

Bioactive metabolites from the sponge-derived fungus Penicillium crustosum SCSIO 41442

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