南沙珊瑚礁区波纹钩鳞鲀(Balistapus undulatus)食性和营养级分析

doi:10.11978/2021008

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (1): 7-14.doi: 10.11978/2021008CSTR: 32234.14.2021008

南沙珊瑚礁区波纹钩鳞鲀(Balistapus undulatus)食性和营养级分析

张琛^1,²(), 胡思敏^1,³(), 林先智¹, 周天成^1,², 黄晖^1,^3,⁴, 刘胜^1,³()

1. 中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东省应用海洋生物学重点实验室, 广东广州 510301
2. 中国科学院大学, 北京 100049
3. 三亚海洋科学综合(联合)实验室, 海南省热带海洋生物技术重点实验室, 三亚中科海洋研究院, 海南三亚 572000
4. 海南三亚海洋生态系统国家野外科学观测研究站, 中国科学院海南热带海洋生物实验站, 海南三亚 572000

收稿日期:2021-01-15 修回日期:2021-04-17 出版日期:2022-01-10 发布日期:2021-04-20
通讯作者: 胡思敏,刘胜
作者简介:张琛(1997—), 男, 甘肃省陇南市人, 硕士研究生, 从事鱼类摄食生态学研究。email: zhangchen183@mails.ucas.ac.cn
基金资助:
中国科学院战略性先导科技专项(XDA13020104);国家自然科学基金项目(41806188);国家重点研发计划(2016YFC0502800);广东省省级科技计划项目(2020B1212060058)

Diet and trophic level analysis of triggerfish (Balistapus undulatuse) in coral reefs of Nansha

ZHANG Chen^1,²(), HU Simin^1,³(), LIN Xianzhi¹, ZHOU Tiancheng^1,², HUANG Hui^1,^3,⁴, LIU Sheng^1,³()

1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. CAS-HKUST Sanya Joint Laboratory of Marine Science Research, Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya Institute of Oceanology, SCSIO, Sanya 572000, China
4. Sanya National Marine Ecosystem Research Station, Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya 572000, China

Received:2021-01-15 Revised:2021-04-17 Online:2022-01-10 Published:2021-04-20
Contact: HU Simin,LIU Sheng
Supported by:
The Strategic Priority Research Program of the Chinese Academy of Sciences(XDA13020104);National Natural Science Foundation of China(41806188);National Key Research and Development Project of China(2016YFC0502800);Science and Technology Planning Project of Guangdong Province, China(2020B1212060058)

摘要/Abstract

摘要：

波纹钩鳞鲀(Balistapus undulatus)是一种在热带珊瑚礁海域中广泛分布的杂食性鱼类, 研究其自然环境中的食物组成有助于了解其食物来源及其在生态系统中的功能地位。但目前的认识仅限于他们是珊瑚礁区的海胆捕食者, 对其准确的食物组成和生态功能定位尚不清晰。本研究于2017年夏季在南沙珊瑚礁区采集了波纹钩鳞鲀样品, 通过特异性引物扩增波纹钩鳞鲀消化道中的线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因, 以高通量测序技术分析了其现场食物组成, 并测定了碳、氮稳定同位素以分析其营养级。研究结果显示, 波纹钩鳞鲀摄食的食物种类有13种, 分属于节肢动物门(Arthropoda)、脊索动物门(Chordata)和环节动物门(Annelida)。其中最主要的食物来源是节肢动物门扇蟹科的蟹类, 如滑面蟹(Etisus sp.)、Luniella pubescens、皱纹花瓣蟹(Liomera rugata)等, 分别占61.8%、6.7%和1.8%。鱼类也有一定的贡献, 占总食物序列的23.5%。同位素结果显示其营养级为3.71±0.07, 与分子检测的结果相符。研究结果表明波纹钩鳞鲀的主要食物是小型甲壳类动物以及植食性鹦嘴鱼, 这拓展了以往对波纹钩鳞鲀所扮演生态角色的认识。

关键词: 波纹钩鳞鲀(Balistapus undulatus), 食性, 高通量测序, 稳定同位素, 营养级, 珊瑚礁

Abstract:

As an omnivorous and widespread fish in tropical coral reefs, Balistapus Undulatus is an important sea urchins predator according to previous observations. However, the accurate food composition and ecological function of B. Undulatus remain unclear due to the lack of food composition information in natural environment. Here, we studied samples of triggerfish collected from the coral reef of Nansha in the summer of 2017. We used the high-throughput sequencing method targeted on the Cytochrome c. Oxidase subunit I (COI) gene and stable isotopes method to analyze the gut content composition of B. Undulatus, and conducted trophic levels analysis. Thirteen species were detected, including Arthropoda, Chordata and Annelida. Crabs of the Xanthidae family were the most important food item, such as Etisus sp., Luniella pubescens and Liomera rugata, accounting for 61.8%, 6.7% and 1.8%, respectively. Fish also made a contribution, accounting for 23.5% of the total food sequence. The isotopic results show that the trophic level was 3.71±0.07, which was consistent with the results of molecular detection. The results show that the main food of triggerfish (B. Undulatus) was small crustaceans and herbivore parrotfish. This study deepens our understanding of the ecological role of triggerfish.

Key words: Balistapus undulatus, diet, high-throughput sequencing, stable isotope, trophic level, coral reef

中图分类号:

P735.12

张琛, 胡思敏, 林先智, 周天成, 黄晖, 刘胜. 南沙珊瑚礁区波纹钩鳞鲀(Balistapus undulatus)食性和营养级分析[J]. 热带海洋学报, 2022, 41(1): 7-14.

ZHANG Chen, HU Simin, LIN Xianzhi, ZHOU Tiancheng, HUANG Hui, LIU Sheng. Diet and trophic level analysis of triggerfish (Balistapus undulatuse) in coral reefs of Nansha[J]. Journal of Tropical Oceanography, 2022, 41(1): 7-14.

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编号	体重/g	体长/cm	饱食度	消化道内容物	高通量测序	同位素分析	δ¹⁵N值/‰	δ¹³C值/‰	营养级
Balun1	118.5	14.0	30%	海胆棘刺	√	√	8.94	-15.70	3.63
Balun2	120.9	14.0	20%	-	√	√	9.60	-15.96	3.82
Balun3	97.9	12.5	20%	贝、螺	√	√	9.13	-16.95	3.69
Balun4	60.1	10.5	30%	贝、头足类	√
Balun5	188.6	15.3	50%	大型藻类	√	√	9.18	-16.08	3.70
Balun6	157.7	14.0	60%	蟹、螺、海胆、藻类	√	√	9.15	-15.79	3.69
Balun7	93.6	12.0	40%	沙子、贝、海胆	√

南沙珊瑚礁区波纹钩鳞鲀(Balistapus undulatus)食性和营养级分析

Diet and trophic level analysis of triggerfish (Balistapus undulatuse) in coral reefs of Nansha

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