Journal of Tropical Oceanography ›› 2018, Vol. 37 ›› Issue (4): 45-51.doi: 10.11978/2017109CSTR: 32234.14.2017109

• Orginal Article • Previous Articles     Next Articles

Genetic variations among Siganus oramin populations in coastal waters of southeast China based on mtDNA control region sequences

Xiaolin HUANG1,2(), Wenjun LI1, Heizhao LIN1,2(), Yukai YANG1,2, Tao LI1,2, Wei YU1,2, Zhong HUANG1,2   

  1. 1. Guangdong Provincial Key Laboratory of Fishery Ecology and Environment; South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China
    2. Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China
  • Received:2017-10-12 Revised:2018-03-31 Online:2018-07-20 Published:2018-07-16
  • Supported by:
    Marine Fishery Science and Technology Industry Projects from Guangdong Province (A201601B10);Guangdong Provincial Key Laboratory of Fishery Ecology and Environment (FEEL-2017-13);Knowledge Innovation Basic Research Project of Shenzhen Science and Technology Program (JCYJ20170817103922921、JCYJ20170817103856495);South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Scientific Research Funds for Central Non-profit Institutes (2016TS01)

Abstract:

In this study, we determined 828 bp mtDNA control region sequences of 100 Siganus oramin individuals collected in Daya Bay, Dapeng Bay, Shaba Bay, and Dongshan Bay where 72 variable sites defined 50 haplotypes. The parameter of haplotype diversity (h) were 0.893±0.053~0.957±0.030, and that of nucleotide diversity (π) was 0.0105±0.0031~0.0179± 0.0031, indicating a peculiarity of high haplotype diversity and low nucleotide diversity, The genetic diversity indexes of four geographical groups were, respectively, 0.0121 (DS), 0.0169 (DY), 0.0107 (DP), and 0.0184 (SB). A combination of the significant negative neutral test values of Fu’s Fs (-16.7079, p<0.01), the mismatch distribution pattern, and the network analysis revealed that a historical population expansion occurred at about 38300 years ago. The indices of genetic distance among populations (0.0112~0.0172) were at a similar level as the indices of genetic distance within populations (0.0107~0.0184). The overall genetic differentiation index (Fst) was 0.0066 (p>0.05), and the analysis of molecular variance showed that most of the genetic variations of the four populations was distributed within populations. With the above findings, it is suggested that S. oramin in the coastal waters of Southeast China is in low level of population genetic diversity and should receive effective protection.

Key words: mitochondrial D-loop, Siganus oramin, genetic diversity

CLC Number: 

  • P735.541