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Journal of Tropical Oceanography

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Secondary metabolites of the soft coral-derived fungus Cladosporium colombiae CSGL 10M10 from the Beibu Gulf and their cardioprotective activities

XIE Jing1, YANG Xiaokun1, HAO Aolin1, TIAN Xiaoyu1, LIN Xiuping2, CHENG Yuanyuan1, ZHANG Cuixian1    

  1. 1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;

    2. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China



  • Received:2026-10-04 Revised:2026-10-16 Accepted:2026-10-22
  • Contact: Zhang, Cui-Xian
  • Supported by:

     National Natural Science Foundation of China (82674913); Special Fund of Guangdong Provincial Department of Natural Resources (GDNRC[2024]25)

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Abstract: This study was conducted to investigate the secondary metabolites and their cardioprotective activities of the soft coral-derived fungus Cladosporium colombiae CSGL 10M10, collected from the Beibu Gulf. The secondary metabolites were isolated and purified by silica gel column chromatography, octadecylsilyl silica (ODS) column chromatography, and semi-preparative high-performance liquid chromatography (HPLC). The structures of the isolated compounds were elucidated on the basis of High Resolution Electrospray Mass Spectrometry (HR-ESI-MS), 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy, optical rotatory dispersion(ORD) and literature comparison. A total of 26 compounds were obtained and identified as Cyclo(Gly-L-Pro) (1), Cyclo(L-Pro-L-Phe) (2), Cyclo(D-Pro-D-Phe) (3), Brevianamide F (4), Cyclo(L-Trp-L-Tyr) (5), Cyclo(L-Leu-L-Tyr) (6), Cyclo(D-Phe-L-Ser) (7), Cyclo(L-Leu-L-Val) (8), Cyclo(L-Ile-L-Val) (9), Phenylacetamide (10), Uracil (11), Genistein (12), Formononetin (13), 3-Hydroxybutan-2-yl 2-hydroxy-3-phenylpropanoate (14), 4-Hydroxyphenylacetic acid butanediol ester (15), Crypticin B (16), Papuline (17), (2-Hydroxyphenyl) acetic acid methyl ester (18), Cinnamic acid (19), 4-Methoxyphenylacetic acid (20), Phenyllactic acid (21), Protocatechuic acid (22), 2-Methylbenzoic acid (23), Benzoic acid (24), Melongenalactone A (25), and Hexadecanoic acid (26). Compound 25 was isolated from the genus Cladosporium for the first time. Except for compounds 2 and 19, all other compounds were isolated from the Cladosporium colombiae strain for the first time. Bioactivity assays of the 24 compounds in normal H9c2 cardiomyocytes and the oxygen-glucose deprivation/reoxygenation (OGD/R)-induced H9c2 cardiomyocyte injury model revealed that compound 20 exhibited cardioprotective activity comparable to that of the positive control diazoxide. In summary, this research enriches the structural diversity of secondary metabolites from marine-derived Cladosporium fungus and provides candidate molecules and experimental evidence for the subsequent development of marine fungus-derived cardioprotective lead compounds.

Key words: soft coral-derived fungusCladosporium colombiae, nitrogen-containing compounds, aromatic phenolic acids, secondary metabolites, cardioprotective activity