Journal of Tropical Oceanography ›› 2020, Vol. 39 ›› Issue (3): 86-97.doi: 10.11978/2019129CSTR: 32234.14.2019129
• Marine Biology • Previous Articles Next Articles
Morphology, phylogeny and toxicity of Coolia tropicalis (Dinophyceae) from the Xisha Islands, China
Lifen HUANG1, Qun LI1, Songhui LÜ1(
), Liang ZHANG2, Xuedong XIE2
- 1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China
2. Guangdong environmental monitoring center, Guangzhou 510362, China
-
Received:2019-12-11Revised:2020-02-03Online:2020-05-10Published:2020-05-19 -
Contact:Songhui Lü E-mail:lusonghui1963@163.com -
Supported by:Foundation item: National Natural Science Foundation of China(41876173);Foundation item: National Natural Science Foundation of China(41706126);Science & Technology Basic Resources Investigation Program of China(2018FY100200);Science & Technology Basic Resources Investigation Program of China(2018FY100100)
Cite this article
Lifen HUANG, Qun LI, Songhui LÜ, Liang ZHANG, Xuedong XIE. Morphology, phylogeny and toxicity of Coolia tropicalis (Dinophyceae) from the Xisha Islands, China[J].Journal of Tropical Oceanography, 2020, 39(3): 86-97.
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Fig. 1
Map of sampling sites"
Fig. 1
Tab. 1
Sampling information"
| 株系号 | 采样时间 | 采样点 | 经纬度 | 附着基质 |
|---|---|---|---|---|
| 2XS73 | 2016-06-11 | 银屿岛(S1) | 16°34'15.39"N, 111°42'13.71"E | 死珊瑚, 少量活珊瑚 |
| 3XS45 | 2016-06-12 | 鸭公岛(S2) | 16°33'30.29"N, 111°40'40.56"E | 死珊瑚 |
| XS554 | 2015-06-11 | 永兴岛(S3) | 16°33'30.29"N, 111°40'40.56"E | 礁岩底质 |
| 5XS15 | 2015-06-11 | 赵述岛(S4) | 16°57'18.73"N, 112°15'58.50"E | 礁石底, 死珊瑚 |
Tab. 1
Fig. 2
Light microscope (LM) images of Coolia tropicalis. a) Cells showing spherical-shaped; b) the arrangement of chloroplasts; c) the U-shaped nucleus; d) ventral view showing cingulum and sulcus"
Fig. 2
Fig. 3
Scanning electron microscope (SEM) images of Coolia tropicalis. a) Lateral view; b) close-up of APC; c) apical view of the epitheca; d) apical view of the epitheca; e) antapical view of the hypotheca; f) ventral view"
Fig. 3
Fig. 4
Thecal plate tabulation of C. tropicalis based on original descriptions. (a, b) This study; (c, d) redrawn from Faust(1995); (e, f) redrawn from Mohammad-Noor et al (2013); (a, c, e) apical view of the epitheca; (b, d, f) antapical view of the hypotheca"
Fig. 4
Fig. 5
Molecular phylogenetic tree based on the D1-D3 region of LSU rDNA with Ostreopsis ovata as outgroups. The numbers at nodes indicate the maximum likelihood booststrap (BS) values (100) and Bayesian Inference posterior probability (PP) values (1), representing the levels of support"
Fig. 5
Tab. 2
Genetic distances of Coolia among different species based on LSU rDNA"
| 种名 | Coolia tropicalis | Coolia palmyrensis | Coolia malayensis | Coolia monotis | Coolia santacroce | Coolia guanchica |
|---|---|---|---|---|---|---|
| C. tropicalis | ||||||
| C. palmyrensis | 0.421 | |||||
| C. malayensis | 0.434 | 0.146 | ||||
| C. monotis | 0.450 | 0.170 | 0.117 | |||
| C. santacroce | 0.428 | 0.098 | 0.090 | 0.086 | ||
| C. guanchica | 0.365 | 0.394 | 0.431 | 0.425 | 0.361 | |
| C. canariensis | 0.392 | 0.354 | 0.396 | 0.416 | 0.359 | 0.187 |
Tab. 2
Fig. 6
Molecular phylogenetic tree based on the ITS region with Ostreopsis ovata as outgroups. The numbers at nodes indicate the maximum likelihood booststrap (BS) values (100) and Bayesian Inference posterior probability (PP) values (1), representing the levels of support"
Fig. 6
Tab. 3
Genetic distances of Coolia among different species based on ITS region"
| 种名 | Coolia tropicalis | Coolia canariensis | Coolia palmyrensis | Coolia santacroce | Coolia monotis |
|---|---|---|---|---|---|
| C. tropicalis | |||||
| C. canariensis | 0.552 | ||||
| C. palmyrensis | 0.553 | 0.798 | |||
| C. santacroce | 0.477 | 0.737 | 0.397 | ||
| C. monotis | 0.428 | 0.577 | 0.379 | 0.201 | |
| C. malayensis | 0.539 | 0.651 | 0.379 | 0.209 | 0.128 |
Tab. 3
Fig. 7
The death rate of A. salina exposed to crude toxin extract of C. tropicalis within 72 h. a) XS554; b) 5XS15"
Fig. 7
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