印度洋深海来源真菌Penicillium sp. SCSIO 06871次级代谢产物研究

doi:10.11978/2024003

Abstract

Abstract:

The rice fermentation products of the fungus Penicillium sp. SCSIO 06871 from the deep-sea Indian Ocean were studied in this paper. The extract was isolated and purified by silica, Sephadex LH-20, and reversed phase octadecylsilyl (ODS) column chromatography, as well as semipreparative high performance liquid chromatography (HPLC). Eight known compounds, including sydowinin B (1), mrlactone1 (2), AGI-B4 (3), methyl cordyol C (4), diorcinol (5), 11-dehydrosydonic acid (6), (+)-sydonic acid (7), and methyl indole-3-acetate (8) were obtained and identified by analyzing their nuclear magnetic resonance (NMR) spectra, mass spectrum (MS) spectra and specific rotation values. Compounds 4, 5 and 8 showed different degree of inhibitory activities against multiple pathogenic bacteria. Also, compounds 5-8 all showed inhibitory activities against α-glycosidase.

Key words: deep-sea-derived fungus, Penicillium sp., secondary metabolite, antibacterial, inhibitory activity against α-glycosidase

CLC Number:

Q936

PANG Xiaoyan, LI Xiaolin, WANG Junfeng, TIAN Xinpeng, XU Shihai, LIU Yonghong. Study on the secondary metabolites from Penicillium sp. SCSIO 06871 of the deep-sea Indian Ocean[J].Journal of Tropical Oceanography, 2024, 43(6): 190-195.

Figures/Tables 2

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Tab. 1

References 16

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仪器/试剂	型号/规格	品牌
立式自动电热压力蒸汽灭菌锅	110L	合肥华泰医疗器械有限公司
恒温摇床	HYG-C三层	北京博宇宝威实验设备有限公司
超声仪	KQ-250DB 型	巩义市予华仪器有限公司
旋转蒸发仪	EYELA N-1000型	上海爱郎仪器有限公司
循环水真空泵	SHZ-CB型	巩义市予华仪器有限公司
柱层析硅胶/薄层层析硅胶	200~300目/ GF 254	烟台江友硅胶开发有限公司
中压制备色谱仪	LC3000型	北京慧德易科技有限责任公司
高效液相色谱仪	半制备型	日本Hitachi 公司
色谱柱1	C-18, 250×10mm, 5μm	日本YMC公司
超导核磁共振仪	AVANCE-500MHz	德国 Bruker 公司
低分辨离子阱质谱仪	amaZon SL	德国 Bruker 公司
旋光光谱仪	MCP 500	奥地利Anton Paar公司
酶标仪	Enspire	新加坡PerkinElmer公司
DMSO-d₆	0.6mL	美国 CIL公司
麦芽提取粉	400g/瓶	广州环凯微生物科技有限公司
海盐	500g/袋	购于市场
大米	5kg/袋	购于市场
胰化蛋白胨	500g/瓶	英国OXOID公司
酵母提取粉	400g/瓶	广州环凯微生物科技有限公司
氨苄西林	5g/瓶	北京兰杰柯科技有限公司
α-葡萄糖苷酶	S10050-100U	上海源叶生物科技有限公司
阿卡波糖	20mg/瓶	北京索莱宝科技有限公司
4-硝基苯基α-D-吡喃葡萄糖苷	1g/瓶	上海麦克林生化科技股份有限公司
石油醚、乙酸乙酯等试剂	分析纯	天津市大茂化学试剂厂
甲醇、乙腈试剂	色谱纯	上海星可高纯溶剂有限公司

Study on the secondary metabolites from Penicillium sp. SCSIO 06871 of the deep-sea Indian Ocean

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