红树植物拟海桑及其亲本的根际细菌群落特征分析

doi:10.11978/2021114

Abstract

Abstract:

Plant rhizosphere microbes can be transferred directly from mother to offspring, these vertically-transmitted organisms can affect host phenotype. Field investigation revealed that Sonneratia × gulngai, a natural hybrid of mangrove plants of the genus Sonneratia, usually grew stronger than its parents, and showed stronger survival advantage. In order to explore the reasons for this phenomenon, the offspring's microbiome composition (S. × gulngai) was compared with its parents S. caseolaris and S. alba by the bacterial 16S rRNA gene high-throughput sequencing technology. The sediment samples were collected from the Dongzhaigang Mangrove National Nature Reserve of Hainan Island. The result showed the rhizosphere bacterial communities of the three mangroves were highly diverse and rich, and distributed into 30 phyla, 242 families and 351 genera. Proteobacteria contributed to 40% of all reads and constituted the predominant members, most of the inherited rhizosphere microbiome belonged to this phylum. Significant differences were also observed at the phylum level. In offspring S. ×gulngai, the abundance of Actinobacteria and Acidobacteria was 4.3% and 6.5% respectively, which are significantly higher than parent's 1%~2%; Thermi species were enriched to be dominant populations with relative abundances of 19.8% in the parent S. alba, but the numbers was only 1% in the offspring S. × gulngai, and found Thermotogae phylum (5%) was absent in the rhizosphere bacterial community of S.× gulngai. In short, microbiome composition differed more strongly between offspring S. × gulngai and the parent S. alba than S. caseolaris. The analysis of soil physical and chemical properties found that the soil total nitrogen (TN) content of S. × gulngai was significantly lower than that of the parent, with a difference of more than 3 times. Correlation analysis showed that the concentration of TN was significantly negatively correlated with the abundance of Planctomycetes, Actinobacteria, and Acidobacteria. Through functional prediction, some functional genes related to the basic metabolism in the offspring S. × gulngai microbial community were greatly enriched, S.× gulngai's metabolic capacity was enhanced. These findings showed that the selectively inherited some the rhizosphere soil microorganisms from their parents, which made the offspring's microbiome composition more reasonable and maintained higher diversity, some rhizosphere growth-promoting bacteria particularly enriched and increased the basal metabolic capacity, these changes promoted S. × gulngai to grow better than its parent.

Key words: mangrove, Sonneratia, rhizosphere microbial, bacterial community, diversity

CLC Number:

S714.3

YE Jincheng, CHEN Yiqing, GAO Lin, ZHOU Xianjiao, ZHONG Cairong, ZHANG Ying, WANG Yun. Analysis of rhizosphere bacterial community characteristics of mangrove plant Sonneratia × gulngai and its parents[J].Journal of Tropical Oceanography, 2022, 41(6): 75-89.

Figures/Tables 9

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样品	OTUs	测序总量
Sc1	6333	38105
Sc2	5987	37913
Sc3	6059	38142
Sal1	1037	39702
Sal2	1905	37969
Sal3	1336	37147
Sg1	5264	39259
Sg2	6409	39784
Sg3	6975	39264

红树物种	香农维纳指数	OTUs观测量	Chao1指数	Ace指数	辛普森指数
Sc	10.44±0.12^a	5196.67±43.59^a	7482.27±197.88^a	7883.287±140.61^a	1±0^a
Sg	10.53±0.24^a	5353.33±562.37^a	6882.33±769.47^a	7231.98±801.72^a	1±0^a
Sal	5.65±0.56^b	1289.67±357.69^b	2229.13±464.49^b	2363.41±533.58^b	0.93±0.02^b

理化指标	样品
理化指标	Sc	Sg	Sal
TC/(mg.g^-1)	24.33±2.07^a	13.17±0.60^b	15.60±2.30^b
TN/(mg.g^-1)	4.33±0.06^b	1.21±0.05^c	4.64±0.04^a
TP/(mg.g^-1)	0.34±0.02^b	0.72±0.04^a	0.67±0.03^a
AK/(mg.g^-1)	0.51±0.01^b	0.34±0.02^c	0.67±0.01^a
C/N	5.62±0.52^b	10.82±0.13^a	3.36±0.52^c
N/P	12.45±0.67^a	1.68±0.03^c	6.93±0.24^b

细菌门	中文名	TC	TN	TP	AK	C/N	N/P
Proteobacteria	变形菌门	-0.817**	0.217	0.700*	0.159	-0.283	-0.667*
Chloroflexi	绿弯菌门	0.433	-0.467	-0.517	-0.402	0.433	0.467
Planctomycetes	浮霉菌门	-0.417	-0.917**	0.333	-0.854**	0.883**	-0.433
Bacteroidetes	拟杆菌门	-0.233	0.65	-0.05	0.678*	-0.817**	0.1
Chlorobi	绿菌门	0.35	0.967**	-0.267	0.879**	-0.933**	0.383
Gemmatimonadetes	出芽单胞菌门	0.1	-0.5	0.083	-0.770*	0.6	-0.1
WS3		0.417	-0.483	-0.533	-0.418	0.417	0.483
Spirochaetes	螺旋体门	0.433	-0.383	-0.55	-0.385	0.35	0.533
Acidobacteria	酸杆菌门	-0.433	-0.933**	0.3	-0.879**	0.850**	-0.417
Firmicutes	厚壁菌门	-0.117	0.717*	0.4	0.527	-0.533	-0.267
Actinobacteria	放线菌门	-0.45	-0.867**	0.383	-0.904**	0.800**	-0.483
Thermi	栖热菌门	0.317	0.850**	-0.433	0.887**	-0.917**	0.5
Thermotogae	热袍菌门	0.356	0.881**	-0.39	0.877**	-0.915**	0.492

Analysis of rhizosphere bacterial community characteristics of mangrove plant Sonneratia × gulngai and its parents

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