三亚湾珊瑚来源虫黄藻不同株系微环境中微生物群落结构的差异比较分析

doi:10.11978/2022013

Abstract

Abstract:

Zooxanthellae, bacteria, and reef—building corals have a close symbiotic relationship, but the phycosphere bacterial community of zooxanthellae is poorly studied. In this study, six strains of zooxanthellae from five genera were cultured in vitro, among which two were free-living zooxanthellae strains (type E) and four were anchorage-dependent living zooxanthellae strains (type A—D). Bacterial community analysis was performed by collecting three different particle size fractions (0.2-3μm, >3μm, and settling algae cells). Results showed that the phycosphere bacterial communities between the two lifestyle algal strains were significantly different. The bacterial species richness in anchorage-dependent living strains was significantly higher than that in free-living strains. Seven bacterial genera were observed to be present in all the algal strains, representing the core bacterial communities of zooxanthellae. Comparing the core bacterial communities in three particle size samples, we found that the core bacterial communities in >3μm samples and in 0.2-3μm samples are highly similar, but both were significantly different from the “Settling” samples.

Key words: symbiodinium, coral, symbiotic relationship, phycosphere bacterial community, algae strain

CLC Number:

Q938

HUANG Sijun, QIU Chen, LONG Chao, LONG Lijuan. Phycosphere microbial communities of zooxanthellae cultures isolated from corals in Sanya Bay, South China Sea[J].Journal of Tropical Oceanography, 2022, 41(6): 90-104.

Figures/Tables 9

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藻株	宿主拉丁文名(中文名)	ITS2基因型	藻株分类(属级)
SYSC-2-1	Acropora tenuis (柔枝鹿角珊瑚)	C1	Cladocopium (C型)
SYSC-2-8	Acropora tenuis (柔枝鹿角珊瑚)	E101	Effrenium (E型)
SYSC-14-11	Galaxea fascicularis (丛生盔形珊瑚)	E101	Effrenium (E型)
SYSC-17-3	Galaxea fascicularis (丛生盔形珊瑚)	A6	Symbiodinium (A型)
SYSC-24-3	Platygyra verwyi (小叶扁脑珊瑚)	D1	Durusdinium (D型)
SYSC-28-9	Pavona decussate (十字牡丹珊瑚)	B1	Breviolum (B型)

藻株1	藻株2	两藻株共有ASVs数(取交集)/两藻株总ASVs数(取并集)	所占比例/%
24-3	28-9	35/76	46.05
17-3	24-3	33/73	45.21
17-3	28-9	30/71	42.25
2-8	14-11	16/42	38.10
2-1	28-9	23/82	28.05
2-1	24-3	24/86	27.91
17-3	2-1	20/80	25.00
14-11	2-1	14/62	22.58
2-8	24-3	15/71	21.13
14-11	28-9	11/66	16.67
2-8	24-3	13/79	16.46
14-11	17-3	10/62	16.13
14-11	24-3	11/71	15.49
2-8	28-9	10/77	12.99
2-8	17-3	9/73	12.33

Phycosphere microbial communities of zooxanthellae cultures isolated from corals in Sanya Bay, South China Sea

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