Journal of Tropical Oceanography ›› 2020, Vol. 39 ›› Issue (1): 66-73.doi: 10.11978/2019021CSTR: 32234.14.2019021
• Marine Biology • Previous Articles Next Articles
Dynamic characteristics of microtubule-dependent transport of Singapore Grouper Iridovirus in host cells
WANG Liqun1,2, WANG Shaowen3, WANG Hongda4, QIN Qiwei3(
)
- 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. College of Marine Sciences, South China Agricultural University, Guangzhou 510642
4. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022, China
-
Received:2019-03-03Revised:2019-04-22Online:2020-01-20Published:2020-01-09 -
Contact:Qiwei QIN E-mail:qinqw@scau.edu.cn -
Supported by:National Natural Science Foundation of China(41606183);Natural Science Foundation of Guangdong Province, China(2016A030310111);National Key R&D Program of China(2017YFA0505300);National Natural Science Foundation of China(21727816);National Natural Science Foundation of China(21721003);National Natural Science Foundation of China(21525314)
CLC Number:
- P735
Cite this article
WANG Liqun, WANG Shaowen, WANG Hongda, QIN Qiwei. Dynamic characteristics of microtubule-dependent transport of Singapore Grouper Iridovirus in host cells[J].Journal of Tropical Oceanography, 2020, 39(1): 66-73.
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Fig. 1
Single virus moved bi-directionally along microtubule. a) Confocal images of single virus moved from cell membrane to MTOC along microtubules. Scale bar is 10 μm. The arrow indicates the virus particle moving on microtubule. The circle indicates MTOC. b) Confocal images of single virus moved from MTOC to cell membrane along microtubules. The arrow indicates the virus particle moving on microtubule. The circle indicates MTOC. c) The trajectory of the virus particle shown in (a) and (b). d) The instantaneous speed of the virus particle shown in (a) and (b). e) The MSD-time plots of the virus particle shown in (a) and (b). The red lines are fitted to the equation LMSD=4Dt+(Vt)2 (LMSD is the average square displacement distance, D and V are the diffusion efficient and mean speed of the particles, respectively). The black lines indicate the MSD data."
Fig. 1
Fig. 2
Single virus decelerated near the intersection of microtubules and moved along the microtubule sequentially. a) Confocal images of single virus limited at an intersection of microtubules. The arrow indicates the virus particle moving on microtubule; b) the trajectory of the virus particle shown in (a); c) the instantaneous speed of the virus particle shown in (a); and d) the MSD-time plots of the virus particle shown in (a)"
Fig. 2
Fig. 3
Single virus limited at the intersection of microtubules. a) Confocal images of single virus limited at an intersection of microtubules. The arrow indicates the virus particle moving on microtubule; b) the trajectory of the virus particle shown in (a); c) the instantaneous speed of the virus particle shown in (a); and d) the MSD-time plots of the virus particle shown in (a)"
Fig. 3
Fig. 4
The morphology of microtubule altered by SGIV. The arrow indicates viral factory"
Fig. 4
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