三份南海岛礁珊瑚砂样品中可培养细菌多样性

doi:10.11978/2021050

Abstract

Abstract:

There are a large number of uncultured microbial resources in coral reef environments. Microbial diversity research will help us understand their ecological functions, and also help develop and utilize microbial resources. In this study, a total of 349 pure bacterial strains were obtained by selective isolation media with multiple oligotrophic components, from three samples collected from three coral reef islands in the South China Sea. Based on 16S rRNA gene sequence analysis, we found the cultivable bacteria belong to four phyla of Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes; and they spread into six class, 26 orders, 43 families, 73 genera, and 134 species. There are 18 potential new taxa with 16S rRNA gene sequence similarities less than 97%. The dominant group is Actinobacteria, which accounts for 60% of all isolates. In this study, the improved and optimized oligotrophic media were used, which showed better isolation results including the composition of the microbial community and rare or new taxa in every sample. The results showed that the cultivable microbial resources are rich and diverse in coral reef island environments of the South China Sea, and the microbial community has comprehensive functions for material and energy metabolism; and the proportion is higher to obtain potential new taxa. This research has laid a good foundation for the methods to mine microbial resources in coral reef islands; it also accumulated abundant and rare microbial resources for the deep development and application in the future.

Key words: Coral reef island, oligotrophic medium, cultivable bacterial diversity, microbial resources

CLC Number:

P735.51

LI Cun, CUI Linqing, YANG Hongqiang, LONG Lijuan, TIAN Xinpeng. Diversity of cultured bacteria isolated from three coral reef sediments in South China Sea[J].Journal of Tropical Oceanography, 2022, 41(2): 149-158.

Figures/Tables 10

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References 21

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培养基名称	培养基成分/(g·L^-1)
5% 2216	蛋白胨5.0, 酵母浸粉1.0, 柠檬酸铁0.1, NaCl 19.45, MgCl₂ 5.98, Na₂SO₄ 3.24, CaCl₂ 1.8, KCl 0.55, Na₂CO₃ 0.16, KBr 0.08, SrCl₂ 0.034, H₃BO₃ 0.022, Na₂O·SiO₂ 0.004, NaF 0.0024, NH₄NO₃ 0.0016, Na₂HPO₄ 0.008, 稀释20倍, 琼脂15. 0
5% AIA	酪蛋白酸钠2.0, 天冬酰胺0.1, 丙酸钠4.0, K₃PO₄ 0.5, MgSO₄ 0.1, FeSO₄ 0.001, 沸水浴1min后加入甘油5.0, 稀释20倍, 琼脂15. 0
5% R2A	胰蛋白胨0.25, 酸水解酪蛋白0.5, 酵母浸粉0.5, 可溶性淀粉0.5, K₂HPO₄ 0.3, MgSO₄ 0.1, 丙酮酸钠0.3, 蛋白胨0.25, 葡萄糖0.5, 稀释20倍, 琼脂15. 0
SN	NaNO₃ 0.75, K₂HPO₄ 0.0159, EDTA二钠0.0056, Na₂CO₃ 0.0104, VitaminB₁₂ 0.001(过滤除菌), 1×10^-6微量盐(乙酸6.25, 柠檬酸铁铵6.0, MnCl₂·4H₂O 1.4, Na₂MoO₄·2H₂O 0.39, Co(NO₃)₂·6H₂O 0.025, ZnSO₃·7H₂O 0.222, 单独灭菌), 琼脂15. 0
10%菌液琼脂	离心收集菌株Gaiella occulta F2-233^T的发酵液100mL, 过滤除菌, 加入900mL纯水, 琼脂15. 0

样品名称	Shannon指数	Simpson指数	Invsimpson指数	Chao1指数	ACE指数
Y11	2.39	0.85	6.78	53.00	42.54
BK-S	3.83	0.95	20.40	182.67	259.60
BS2-S	3.81	0.97	30.07	169.11	176.52

菌种编号	合并菌株数	最相似菌种	16S RNA序列相似性	新分类单元
SCSIO 63424	1	Arboricoccus pini	89.88	潜在新属
SCSIO 63409	3	Inquilinus limosus	92.80	潜在新属
SCSIO 63602	1	Nocardioides mesophilus	93.64	潜在新属
SCSIO 63346	1	Brevibacterium ammoniilyticum	94.61	潜在新属
SCSIO 63385	1	Gordonia terrae	94.74	潜在新属
SCSIO 63263	3	Brevibacterium casei	95.07	潜在新属
SCSIO 63650	1	Brachybacterium paraconglomeratum	95.66	潜在新属
SCSIO 63659	6	Nocardioides iriomotensis	96.00	潜在新种
SCSIO 63265	2	Haoranjiania flava	96.19	潜在新种
SCSIO 63649	1	Prauserella aidingensis	96.34	潜在新种
SCSIO 63671	1	Mesorhizobium oceanicum	96.45	潜在新种
SCSIO 63658	1	Prauserella rugosa	96.56	潜在新种
SCSIO 63505	1	Cohnella cellulosilytica	96.58	潜在新种
SCSIO 63329	1	Bacillus dafuensis	96.71	潜在新种
SCSIO 63500	1	Microbacterium oxydans	96.72	潜在新种
SCSIO 63633	1	Maricaulis virginensis	96.77	潜在新种
SCSIO 68054	1	Oceanobacillus iheyensis	97.01	潜在新种
SCSIO 63591	1	Sphingomonas aestuarii	97.02	潜在新种

Diversity of cultured bacteria isolated from three coral reef sediments in South China Sea

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