急性盐度胁迫对斜带石斑鱼Na+/K+-ATP酶及血清应激指标的影响

doi:10.11978/j.issn.1009-5470.2010.04.160

Abstract

Abstract:

Effects of acute salinity stress on plasma indicators and Na+/K+-ATPase activity in gills of Epinphelus coioides juveniles were studied. E. coioides juveniles with body weight (19.59 ±0.25)g in the seawater with salinity of 34‰ were directly transferred to water with salinities at 0‰, 4‰, 14‰, and 24‰, while those in seawater with salinity of 34‰ were taken as the control group. Plasma glucose content, activities of lysozyme, aspartate carbamyl transferase (AST) and Na+/K+-ATPase in gills were determined at 1, 3, 6, 12, and 24h after the transferring of E. coioides. The results showed that Na+/K+-ATPase activity in salinity stress groups displayed a similar trend of increasing first to its peak at 1h, decreasing thereafter, remaining stable at 6h, and being significantly higher than that in the control group later (P<0.05). Plasma glucose content in waters with salinities 24‰ and 14‰ showed a downward trend, and the content in waters with salinities 4‰ and 0‰ exhibited the lowest value at 3h, followed by an upward trend with the peak occurring at 6h and a gradual decrease thereafter. There were no significant differences in AST among waters with salinity of 14‰, 24‰ and 34‰ (P>0.05). AST in waters with salinities 4‰ and 0‰ reached its peak at 6h and 12h, respective, and then decreased. There were significant differences in lysozyme activity among waters with salinity of 24‰, 14‰ and 4‰ at 24h (P<0.05). Lysozyme activity in salinity 0‰ increased first and then declined, with the peak occurring at 6h. In conclusion, E. coioides juveniles had a strong adaptability to ambient salinity stress, and they could adapt themselves at 6h. In waters with salinity at 14‰ and 24‰, E. coioides juveniles showed the weakest response. According to the results of this study, we conclude that E. coioides juveniles can be transferred directly from seawater to water with salinity of 14‰ before the salinity in water is gradually reduced to a prospective salinity value, which can reduce the time of desalination.

Key words: Epinephelus coioides, salinity stress, Na+/K+ -ATP, plasma glucose, AST, lysozyme

YANG Yu-qing,YU De-guang,XIE Jun,YU Er-meng,LI Wang-dong,WANG Guang-. Effects of acute salinity stress on Na+/K+-ATPase activity and plasma indicators of Epinephelus coioides[J].Journal of Tropical Oceanography, 2010, 29(4): 160-164.

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Effects of acute salinity stress on Na+/K+-ATPase activity and plasma indicators of Epinephelus coioides

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