硇洲岛半叶马尾藻中国变种的受精过程观察及其幼孢子体生长条件初探

doi:10.11978/2024119

Abstract

Abstract:

Sargassum hemiphyllum var. chinense J. Agardh is an economic seaweed widely distributed along the coast of the South China Sea. To investigate the sexual reproductive characteristics and the feasibility of artificial seedling propagation of this brown macroalga, this study employed optical microscopy to examine the morphological features of the thallus, male and female receptacles, and the early developmental stages of fertilized eggs in S. hemiphyllum var. chinense from Naozhou Island. The results revealed that the mature thallus of this Chinese variant exhibited a yellow-brown hue with a height of 0.8 to 1 m. It exhibited dioecious traits and possessed discoid holdfasts composed of repetitive bifurcations. The female receptacles were yellow-brown and cylindrical, adorned with numerous surface spines, measuring (0.46 ± 0.05) mm in diameter and (2.50 ± 0.67) mm in length. Conversely, the male receptacles were lighter in color, adopting a rod-like morphology, with a diameter of (1.01 ± 0.18) mm and a length of (9.21 ± 2.36) mm. Eggs released from the female structures exhibited an eight-nucleate one-egg configuration, either ellipsoidal or oval, with an outside diameter of (76.32 ± 10.11) μm. Following ovulation, these eggs adhered to the female structures awaiting fertilization. Sperms released from the male structures were pear-shaped, measuring (27.52 ± 5.43) μm in length and equipped with flagella. At the post-fertilization stage, the majority of fertilized eggs detached from the receptacles, affixing to substrates and undergoing early developmental processes. Within six hours, these fertilized eggs underwent their initial transverse division, subsequently progressing through multiple transverse and longitudinal divisions, and ultimately culminating in the development of landmine-shaped sporophytes within 24 hours, with primary fronds emerging after 28 days. Additionally, this study investigated the indoor use of suitable artificial substrates for the germination of sporophytes and the effect of temperature on the S. hemiphyllum var. chinense growth. Results indicated that, after seven days of culture, the mesh curtain yielded a germination rate of (7.25 ± 3.64) sporophytes per cm², measuring (1.38 ± 0.35) mm in sporophytes’ length. Additional indoor suspension culture experiments revealed a 90% survival rate of sporophytes at the optimal temperature of 25℃, with a thallus growth rate of 90.1 μm·d⁻¹, significantly outperforming other temperatures. These findings provide valuable preliminary data on which to base further studies of the early development and large-scale artificial seedling propagation of S. hemiphyllum var. chinense, thereby facilitating the rapid establishment of natural Sargassum habitats in coastal regions and contributing to the swift rehabilitation of China’s nearshore marine ecosystems.

Key words: Sargassum hemiphyllum var.chinense J. Agardh, sexual reproduction, growth, morphological characteristics

CLC Number:

P735.531

YANG Wencheng, XIE Enyi, LIN Kun, GUO Youyou, WANG Huihui, CHEN Chunli, XIN Rong, CUI Jianjun. Observation of the fertilization process of Sargassum hemiphyllum var. chinense J. Agardhon Naozhou Island and a preliminary study on the optimal growth conditions for the juvenile sporophyte[J].Journal of Tropical Oceanography, 2025, 44(2): 137-146.

Figures/Tables 10

Fig. 1

Fig. 2

Fig. 3

Tab. 1

Fig. 4

Fig. 5

Fig. 6

Tab. 2

Tab. 3

Fig. 7

References 36

[1]	韩军军, 白凯强, 赵明林, 等, 2016. 瓦氏马尾藻有性繁殖和幼孢子体发育的形态学观察[J]. 水生生物学报, 40(6): 1215-1220.
	HAN JUNJUN, BAI KAIQIANG, ZHAO MINGLIN, et al, 2016. Morphological observation of Sargassum vachellianum sexual reproduction and juvenile sporophyte development[J]. Acta Hydrobiologica Sinica, 40(6): 1215-1220 (in Chinese with English abstract).
[2]	贺亮, 2017. 高温胁迫下半叶马尾藻中国变种生理生化响应及其耐热机制的初步研究[D]. 湛江: 广东海洋大学.
	HE LIANG, 2017. Preliminary study on physiological and biochemical responses, heat-tolerance mechanism of Sargassum hemiphyllum var. chinense under high temperature[D]. Zhanjiang: Guangdong Ocean University (in Chinese with English abstract).
[3]	黄冰心, 丁兰平, 谭华强, 等, 2013. 我国沿海马尾藻属(Sargassum)的物种多样性及其区系分布特征[J]. 海洋与湖沼, 44(1): 69-76.
	HUANG BINGXIN, DING LANPING, TAN HUAQIANG, et al, 2013. Species diversity and distribution of genus Sargassum in China seas[J]. Oceanologia et Limnologia Sinica, 44(1): 69-76 (in Chinese with English abstract).
[4]	贾柽, 2012. 基于人工育苗的硇洲马尾藻增殖与移殖[D]. 湛江: 广东海洋大学.
	JIA CHENG, 2012. The proliferation and colonization of Sargassum naozhouense based on the artificial breeding[D]. Zhanjiang: Guangdong Ocean University (in Chinese with English abstract).
[5]	贾柽, 杨彬, 谢恩义, 2012. 莫氏马尾藻繁殖生物学初步研究[J]. 水产科学, 31(10): 616-619.
	JIA CHENG, YANG BIN, XIE ENYI, 2012. Reproductive biology of seaweed Sargassum moclurei[J]. Fisheries Science, 31(10): 616-619 (in Chinese with English abstract).
[6]	姜森颢, 任贻超, 唐伯平, 等, 2017. 我国刺参养殖产业发展现状与对策研究[J]. 中国农业科技导报, 19(9): 15-23. doi: 10.13304/j.nykjdb.2017.0202
	JIANG SENHAO, REN YICHAO, TANG BOPING, et al, 2017. Development status and countermeasures of Apostichopus japonicus culture industry in China[J]. Journal of Agricultural Science and Technology, 19(9): 15-23 (in Chinese with English abstract).
[7]	李爵乾, 廖嘉欣, 谢恩义, 2018. 温度对半叶马尾藻生长及生化组分的影响[J]. 河南农业, (5): 56, 59 (in Chinese).
[8]	林立东, 尚天歌, 张甜甜, 等, 2020. 褐藻羊栖菜繁殖生物学特征和生活史流程的补充研究[J]. 水产学报, 44(4): 581-595.
	LIN LIDONG, SHANG TIANGE, ZHANG TIANTIAN, et al, 2020. Supplementary study on reproductive biology and life cycle of Sargassum fusiforme (Phaeophyceae)[J]. Journal of Fisheries of China, 44(4): 581-595 (in Chinese with English abstract).
[9]	刘玮, 2015. 鼠尾藻群体资源调查及人工繁育的研究[D]. 北京: 中国科学院研究生院(海洋研究所).
	LIU WEI, 2015. Surveys of natural populations and artificial breeding of Sargassum thunbergii[D]. Beijing: Institute of Oceanology, Chinese Academy of Sciences (in Chinese with English abstract).
[10]	骆其君, 2002. 赤潮对羊栖菜产量构成的影响[J]. 中国水产科学, 9(1): 95-96.
	LUO QIJUN, 2002. Effects of red tide on product construction of Hizikia fusiforme[J]. Journal of Fishery Sciences of China, 9(1): 95-96 (in Chinese with English abstract).
[11]	逄少军, 费修绠, 肖天, 等, 2000. 羊栖菜生殖托的离体培养的初步研究[J]. 海洋科学, 24(3): 1-3.
	PANG SHAOJUN, FEI XIUGENG, XIAO TIAN, et al, 2000. Suspended culture of the receptacles of Hizikia fusiformis[J]. Marine Sciences, 24(3): 1-3 (in Chinese).
[12]	曲元凯, 谢恩义, 孙立伟, 等, 2015. 3种马尾藻周年生长和成熟时间以及形态学研究[J]. 安徽农业科学, 43(23): 136-139, 231.
	QU YUANKAI, XIE ENYI, SUN LIWEI, et al, 2015. Study on the annual growth, maturity time and morphology of three species of Sargassum[J]. Journal of Anhui Agricultural Sciences, 43(23): 136-139, 231 (in Chinese with English abstract).
[13]	孙建璋, 庄定根, 陈万东, 等, 2008. 铜藻Sargassum horneri繁殖生物学及种苗培育研究[J]. 南方水产, 4(2): 6-14.
	SUN JIANZHANG, ZHUANG DINGGEN, CHEN WANDONG, et al, 2008. Studies on sexual reproduction and seedling production of the brown alga Sargassum horneri[J]. South China Fisheries Science, 4(2): 6-14 (in Chinese with English abstract).
[14]	孙立伟, 谢恩义, 曲元凯, 2015. 亨氏马尾藻的有性生殖观察[J]. 南方水产科学, 11(1): 62-67.
	SUN LIWEI, XIE ENYI, QU YUANKAI, 2015. Sexual reproduction of Sargassum henslouianum[J]. South China Fisheries Science, 11(1): 62-67 (in Chinese with English abstract).
[15]	孙修涛, 王飞久, 汪文俊, 等, 2010. 基于有性繁殖的鼠尾藻规模化繁育试验[J]. 渔业科学进展, 31(3): 84-91.
	SUN XIUTAO, WANG FEIJIU, WANG WENJUN, et al, 2010. Large scale breeding of Sargassum thunbergii based on sexual reproduction[J]. Progress in Fishery Sciences, 31(3): 84-91 (in Chinese with English abstract).
[16]	田淑娴, 陈万东, 林利, 等, 2014. 繁殖期半叶马尾藻中国变种的形态结构观察[J]. 海洋渔业, 36(2): 107-115.
	TIAN SHUXIAN, CHEN WANDONG, LIN LI, et al, 2014. Morphological observation and structual analysis of Sargassum hemiphyllum (Turn.) var. chinense J. Agardh in breeding season[J]. Marine Fisheries, 36(2): 107-115 (in Chinese with English abstract).
[17]	王惠, 谢恩义, 徐日升, 等, 2019. 硇洲马尾藻Sargassum naozhouense幼孢子体附着及原生态恢复[J]. 广东海洋大学学报, 39(1): 42-48.
	WANG HUI, XIE ENYI, XU RISHENG, et al, 2019. Attachment of young sporophores and restoration in natural rocky habitats of Sargassum naozhouense resources[J]. Journal of Guangdong Ocean University, 39(1): 42-48 (in Chinese with English abstract).
[18]	向玉哲, 2022. 人工藻礁的选型与藻类附着效果的初步研究[D]. 汕头: 汕头大学.
	XIANG YUZHE, 2022. A survey of the distribution characteristics of Sargassum hemiphyllum var. Chinese in four geographical populations and its response to environmental stress under high and low temperatures[D]. Shantou: Shantou University (in Chinese with English abstract).
[19]	邢珊珊, 邹潇潇, 林勇, 等, 2016. 匍枝马尾藻有性繁殖和人工育苗的初步研究[J]. 水产科学, 35(4): 376-380.
	XING SHANSHAN, ZOU XIAOXIAO, LIN YONG, et al, 2016. Sexual reproduction and artificial breeding of brown alga Sargassum polycystum[J]. Fisheries Science, 35(4): 376-380 (in Chinese with English abstract).
[20]	于沛民, 2007. 人工藻礁的选型与藻类附着效果的初步研究[D]. 青岛: 中国海洋大学.
	YU PEIMIN, 2007. The choice of material and shape of artificial alga reefs and the elementary investigation of attachment of algas[D]. Qingdao: Ocean University of China (in Chinese with English abstract).
[21]	曾呈奎, 2000. 中国海藻志. 第三卷褐藻门, 第二册墨角藻目[M]. 北京: 科学出版社.
	ZENG CHENGKUI, 2000. Flora algarum marinarum sinicarum[M]. Beijing: Science Press (in Chinese).
[22]	曾昭钧, 孙立伟, 谢恩义, 2024. 围氏马尾藻周年生长及有性生殖的早期发育研究[J]. 热带海洋学报, 44(1): 44-52.
	ZENG ZHAOJUN, SUN LIWEI, XIE ENYI, 2024. Study on annual growth and early development of sexual reproduction of Sargassum wightii[J]. Journal of Tropical Oceanography, 44(1): 44-52 (in Chinese with English abstract).
[23]	张立宁, 2013. 羊栖菜孢子体繁育及幼苗北方海区渡夏培育的研究[D]. 宁波: 宁波大学.
	ZHANG LINING, 2013. The study on the artificial breed and cultivation for the sporophytes of Sargassum fusiforme and the culturing seedling at the North Sea of Sargassum Fusiforme Setch during summer[D]. Ningbo: Ningbo University (in Chinese with English abstract).
[24]	赵素芬, 2012. 海藻与海藻栽培学[M]. 北京: 国防工业出版社.
	ZHAO SUFEN, 2012. Marine algae and algal culture science[M]. Beijing: National Defense Industry Press (in Chinese).
[25]	AGARDH J G, 1889. Species sargassorum australiae descriptae et dispositae[J]. Kungliga Svenska Vetenskapsakademiens Handlingar, 23: 1-133.
[26]	AJISAKA T, NANG H Q, DINH N H, et al, 1997. Sargassum hemiphyllum (Turner) C. Agardh var. chinense J. Agardh from Vietnam, the Chinese mainland, and Taiwan (Fucales, Phaeophyta)[M]//ABBOTT I A. Taxonomy of economic seaweeds with reference to some pacific species. La Jolla, California: California Sea Grant College Program, University of California: 6.
[27]	CHEANG C C, CHU K H, ANG JR P O, 2008. Morphological and genetic variation in the populations of Sargassum hemiphyllum (Phaeophyceae) in the northwestern Pacific[J]. Journal of Phycology, 44(4): 885-865.
[28]	CHU S H, ZHANG Q S, LIU S K, et al, 2011. Trade-off between vegetative regeneration and sexual reproduction of Sargassum thunbergii[J]. Hydrobiologia, 678(1): 127-135.
[29]	GUIRY M D, 2024. How many species of algae are there? A reprise. Four kingdoms, 14 phyla, 63 classes and still growing[J]. Journal of Phycology, 60(2): 214-228.
[30]	OLIVEIRA E C, ALVEAL K, ANDERSON R J, 2000. Mariculture of the agar-producing gracilarioid red algae[J]. Reviews in Fisheries Science, 8(4): 345-377.
[31]	PANG S J, LIU F, SHAN T F, et al, 2009. Cultivation of the brown alga Sargassum horneri: sexual reproduction and seedling production in tank culture under reduced solar irradiance in ambient temperature[J]. Journal of Applied Phycology, 21(4): 413-422.
[32]	PHILLIPS N, 1995. Biogeography of Sargassum (Phaeophyta) in the Pacific basin[M]//ABBOTT I A. Taxonomy of economic seaweeds. La Jolla, California: California Sea Grant College Program: 107-144.
[33]	SALDARRIAGA-HERNANDEZ S, NÁJERA-MARTÍNEZ E F, MARTÍNEZ-PRADO M A, et al, 2020. Sargassum-based potential biosorbent to tackle pollution in aqueous ecosystems-an overview[J]. Case Studies in Chemical and Environmental Engineering, 2: 100032.
[34]	TERAWAKI T, YOSHIKAWA K, YOSHIDA G, et al, 2003. Ecology and restoration techniques for Sargassum beds in the Seto Inland Sea, Japan[J]. Marine Pollution Bulletin, 47(1-6): 198-201.
[35]	TSENG C K, YOSHIDA T, CHIANG Y M, 1985. East Asiatic species of Sargassum subgenus Bactrophycus J. Agardh (Sargassaceae, Fucales), with keys to the sections and species[M]//ABBOTT I A, NORRIS J N. Taxonomy of economic seaweeds: with reference to some Pacific and Caribbean species. La Jolla, California: California Sea Grant College Program: 1-15.
[36]	YU Y Q, ZHANG Q S, TANG Y Z, et al, 2012. Establishment of intertidal seaweed beds of Sargassum thunbergii through habitat creation and germling seeding[J]. Ecological Engineering, 44: 10-17.

性别	第二分支生殖托总数/个	生殖托长/mm	生殖托直径/mm
雄性	74.17 ± 12.69	9.21 ± 2.36	1.01 ± 0.18
雌性	56 ± 4.08	2.50 ± 0.67	0.46 ± 0.05

基质	幼孢子体附着	幼苗长度/mm	幼苗密度/(indiv.·cm^-2)
牡蛎壳	有	0.92 ± 0.34^a	3.20 ± 2.15^a
布帘	有	1.38 ± 0.35^b	7.25 ± 3.64^b
铝板	无	—	—
塑料板	无	—	—
水泥砖	有	—	—

	第7天			第14天
	20℃	25℃	30℃	20℃	25℃	30℃
存活率/%	75	95	55	75	90	40
生长速率/(μm·d^-1)	-1.4	102.9	-15.7	3.6	90.1	-10.6

Observation of the fertilization process of Sargassum hemiphyllum var. chinense J. Agardhon Naozhou Island and a preliminary study on the optimal growth conditions for the juvenile sporophyte

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 36

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZENG Zhaojun, SUN Liwei, XIE Enyi. Study on the annual growth and early development of sexual reproduction of Sargassum wightii [J]. Journal of Tropical Oceanography, 2025, 44(1): 44-52.
[2]	HAO Yanxia, ZHAO Yuxuan, DENG Liqiu, ZHANG Ruixue, WANG Shuhong. Observation on the asexual reproduction of Phyllorhiza sp. [J]. Journal of Tropical Oceanography, 2025, 44(1): 35-43.
[3]	ZHOU Weihua, LI Yingxin, GUO Yajuan, HUO Jiaxin, SONG Yan, ZHU Qing, YUAN Xiangcheng, LIU Sheng, HUANG Hui. Effects of ocean acidification and warming on the growth and calcification of two reef-building corals* [J]. Journal of Tropical Oceanography, 2024, 43(3): 49-57.
[4]	YANG Fangfang, XIAO Zhiliang, WEI Zhangliang, HUANG Yi, LONG Lijuan. The effects of ocean acidification and warming on the growth and calcification in coralline alga Porolithon cf. onkodes [J]. Journal of Tropical Oceanography, 2023, 42(1): 87-97.
[5]	LIU Shuangshuang, YANG Sheng, LIU Xing, CHEN Qiuxia, WANG Jinwang, GUO Jinmin, WANG Jiayu, WANG Wenqing, WU Weizhi, LIANG Licheng, ZHANG Xiaowei. Morphological characteristics of hypocotyls with different fresh weights of Kandelia obovata and their effects on the seedling growth [J]. Journal of Tropical Oceanography, 2022, 41(6): 56-66.
[6]	WU Weizhi, ZHAO Zhixia, YANG Sheng, LIANG Licheng, Chen Qiuxia, LU Xiang, LIU Xing, ZHANG Xiaowei. The mangrove forest distribution and analysis of afforestation effect in Zhejiang Province [J]. Journal of Tropical Oceanography, 2022, 41(6): 67-74.
[7]	LIU Xiaoju, SHI Qi, TAO Shichen, YANG Hongqiang, ZHANG Xiyang, ZHOU Shengnan. The growth rate of coral Porites at the Zhongbei Ansha of the Zhongsha Atoll and its response to the seawater temperature change in the past 165 Years [J]. Journal of Tropical Oceanography, 2022, 41(5): 64-73.
[8]	CHEN Qiming, LIU Songlin, ZHANG Chi, CUI Lijun, JIANG Zhijian, WU Yunchao, HUANG Xiaoping. Growth characteristics of four representative fishes and their responses to seagrass resource changes in typical tropical seagrass beds of Hainan Island [J]. Journal of Tropical Oceanography, 2020, 39(5): 62-70.
[9]	LIU Yu, WANG Xuehui, DU Feiyan, LIU Bilin, ZHANG Peng, LIU Mengna, QIU Yongsong. Age and growth of Sthenoteuthis oualaniensis based on statolith microstructure in the South China Sea [J]. Journal of Tropical Oceanography, 2019, 38(6): 62-73.
[10]	Jialin AI, Zhimin LI, Jianyong LIU, Yuchun SHEN. Single nucleotide polymorphisms of myostation gene and its association with growth traits in Haliotis diversicolor supertexta [J]. Journal of Tropical Oceanography, 2019, 38(2): 78-85.
[11]	Minhang HU, Tianran CHEN, Wenjing ZHANG. Inter-species and inter-colony differences of Sr/Ca-SST calibration in Porites [J]. Journal of Tropical Oceanography, 2018, 37(6): 74-84.
[12]	Xuejia HE, Huaming YANG, Baidong LYU, Jianqiang YIN. Nucleic acid proxies for ingestion rate, growth and reproduction in copepod Pseudodiaptomus annandalei [J]. Journal of Tropical Oceanography, 2018, 37(2): 26-35.
[13]	Yajuan GUO, Weihua ZHOU, Xiangcheng YUAN, Jianzu LIAO, Lei JIANG, Hui HUANG. Responses of two species of reef-building corals to acidification and dissolved organic carbon enrichment^* [J]. Journal of Tropical Oceanography, 2018, 37(1): 57-63.
[14]	Jiaxing LIU, Linbin ZHOU, Zhixin KE, Gang LI, Rongjun SHI, Yehui TAN. Effect of aluminum on the growth and nitrogen fixation of a marine nitrogen-fixing cyanobacterium: Crocosphaera watsonii [J]. Journal of Tropical Oceanography, 2017, 36(2): 12-18.
[15]	Guojian WEI, Yaoguo LI, Jianshi LIN, Maoxian HE. Verification of QTL and genotype selection effect of QTL for growth-related traits in the Pearl Oyster, Pinctada fucata [J]. Journal of Tropical Oceanography, 2017, 36(2): 19-25.