海水酸化与升温对孔石藻(Porolithon cf. onkodes)生长钙化的影响

doi:10.11978/2022099

Abstract

Abstract:

Calcified macroalgae, an important calcifying group widely distributed in reef ecosystems, plays vital roles in the primary productivity and reef frameworks construction. Furthermore, several species of calcified algae can induce the coral larval settlement, which is closely related to the sustained development of coral reef ecosystem. However, it is not clear how calcified algae will response to ocean acidification and warming. In this study, healthy and bleached Porolithon cf. onkodes were exposed to different pCO₂ (400 µatm, 1200 µatm, 1800 µatm) and temperatures (27 ℃, 30 ℃, 32 ℃) for one month, respectively. The results showed that the growth rate and net calcification rate of living P. onkodes were not significantly affected when the temperature increased from 27 ℃ to 30 ℃, however, these physiological parameters declined significantly at 32 ℃. The net calcification rate decreased from 206.99 nmol·cm^-2·h⁻¹(400 µatm pCO₂+ 27 ℃) to -42.22 nmol·cm^-2·h⁻¹(1200 µatm pCO₂+ 32 ℃). Similarly, the enhancement of pCO₂ also significantly inhibited the growth and calcification in living P. onkodes. Additionally, warming and acidification had interactive effects on the chlorophyll a content and net calcification rate. Compared with living P. onkodes, dead or bleached algal skeletons were more susceptible to warming and acidification. When the temperature was 30 ℃ or the pCO₂ was 1200 µatm, the net dissolution rate of dead algal skeletons increased significantly. Furthermore, the adverse effects of warming were exacerbated when high temperatures coincided with acidification. The results revealed that acidification and warming not only affected the growth and calcification rates of living algae, but also accelerated the dissolution rate of calcium carbonate skeleton, which can affect the coral reef ecosystem. The study may contribute to predict the impacts of climate change on reef ecosystem to protect reef ecosystems.

Key words: ocean acidification, warming, Porolithon cf. onkodes, calcification, growth, coral reef ecosystems

YANG Fangfang, XIAO Zhiliang, WEI Zhangliang, HUANG Yi, LONG Lijuan. The effects of ocean acidification and warming on the growth and calcification in coralline alga Porolithon cf. onkodes[J].Journal of Tropical Oceanography, 2023, 42(1): 87-97.

Figures/Tables 7

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References 57

[1]	雷新明, 黄晖, 黄良民, 2012. 珊瑚礁生态系统中珊瑚藻的生态作用研究进展[J]. 生态科学, 31 (5): 585-590.
	LEI XINMING, HUANG HUI, HUANG LIANGMIN, 2012. Current state of ecological functions of coralline algae in coral reef ecosystem[J]. Ecological Science, 31(5): 585-590. (in Chinese with English abstract)
[2]	雷新明, 黄晖, 练健生, 等, 2019. 中国珊瑚藻的多样性及分布研究现状[J]. 热带海洋学报, 38(4): 30-40. doi: 10.11978/2018117
	LEI XINMING, HUANG HUI, LIAN JIANSHENG, et al, 2019. The diversity and distribution of coralline algae in China: state of knowledge and research[J]. Journal of Tropical Oceanography, 38(4): 30-40. (in Chinese with English abstract) doi: 10.11978/2018117
[3]	李银强, 余克服, 王英辉, 等, 2016. 珊瑚藻在珊瑚礁发育过程中的作用[J]. 热带地理, 36(1): 19-26. doi: 10.13284/j.cnki.rddl.002805
	LI YINQIANG, YU KEFU, WANG YINGHUI, et al, 2016. Review on the coralline algae functions in the development process of coral reef[J]. Tropical Geography, 36(1): 19-26. (in Chinese with English abstract)
[4]	聂磊, 谢子强, 彭丹, 2021. 海水酸化对珊瑚藻生长和钙化作用的影响[J]. 广东海洋大学学报, 41(3): 67-73.
	NIE LEI, XIE ZIQIANG, PENG DAN, 2021. Impact of seawater acidification on growth and calcification characteristics of coralline algae[J]. Journal of Guangdong Ocean University, 41(3): 67-73. (in Chinese with English abstract)
[5]	徐智广, 李美真, 霍传林, 等, 2012. 高浓度CO₂引起的海水酸化对小珊瑚藻光合作用和钙化作用的影响[J]. 生态学报, 32(3): 699-705.
	XU ZHIGUANG, LI MEIZHEN, HUO CHUANLIN, et al, 2012. Effects of CO₂-induced seawater acidification on photosynthesis and calcification in the coralline alga Corallina pilulifera[J]. Acta Ecologica Sinica, 32(3): 699-705. (in Chinese with English abstract) doi: 10.5846/stxb
[6]	ANTHONY K R N, KLINE D I, DIAZ-PULIDO G, et al, 2008. Ocean acidification causes bleaching and productivity loss in coral reef builders[J]. Proceedings of the National Academy of Sciences of the United States of America, 105(45): 17442-17446. doi: 10.1073/pnas.0804478105 pmid: 18988740
[7]	BERGSTROM E, ORDOÑEZ A, HO M, et al, 2020. Inorganic carbon uptake strategies in coralline algae: Plasticity across evolutionary lineages under ocean acidification and warming[J]. Marine Environmental Research, 161: 105107. doi: 10.1016/j.marenvres.2020.105107
[8]	CAMPBELL J E, FISCH J, LANGDON C, et al, 2016. Increased temperature mitigates the effects of ocean acidification in calcified green algae (Halimeda spp. )[J]. Coral Reefs, 35: 357-368. doi: 10.1007/s00338-015-1377-9
[9]	CHAN P T W, HALFAR J, ADEY W H, 2020. Recent density decline in wild-collected subarctic crustose coralline algae reveals climate change signature[J]. Geology, 48(3): 226-230. doi: 10.1130/G46804.1
[10]	COMEAU S, CARPENTER R C, EDMUNDS P J, 2014. Effects of irradiance on the response of the coral Acropora pulchra and the calcifying alga Hydrolithon reinboldii to temperature elevation and ocean acidification[J]. Journal of Experimental Marine Biology and Ecology, 453: 28-35. doi: 10.1016/j.jembe.2013.12.013
[11]	CORNWALL C E, BOYD P W, MCGRAW C M, et al, 2014. Diffusion boundary layers ameliorate the negative effects of ocean acidification on the temperate coralline macroalga Arthrocardia corymbosa[J]. PLoS One, 9(9): e97235. doi: 10.1371/journal.pone.0097235
[12]	CORNWALL C E, DIAZ-PULIDO G, COMEAU S, 2019. Impacts of ocean warming on coralline algal calcification: meta-analysis, knowledge gaps, and key recommendations for future research[J]. Frontiers in Marine Science, 6: 186. doi: 10.3389/fmars.2019.00186
[13]	CORNWALL C E, HARVEY B P, COMEAU S, et al, 2022. Understanding coralline algal responses to ocean acidification: Meta-analysis and synthesis[J]. Global Change Biology, 28(2): 362-374. doi: 10.1111/gcb.v28.2
[14]	CORONADO I, FINE M, BOSELLINI F R, et al, 2019. Impact of ocean acidification on crystallographic vital effect of the coral skeleton[J]. Nature Communications, 10(1): 2896. doi: 10.1038/s41467-019-10833-6 pmid: 31263108
[15]	DAVIES P S, 1989. Short-term growth measurements of corals using an accurate buoyant weighing technique[J]. Marine Biology, 101(3): 389-395. doi: 10.1007/BF00428135
[16]	DIAZ-PULIDO G, ANTHONY K R N, KLINE D I, et al, 2012. Interactions between ocean acidification and warming on the mortality and dissolution of coralline algae[J]. Journal of Phycology, 48(1): 32-39. doi: 10.1111/jpy.2012.48.issue-1
[17]	DOVE S G, BROWN K T, VAN DEN HEUVEL A, et al, 2020. Ocean warming and acidification uncouple calcification from calcifier biomass which accelerates coral reef decline[J]. Communications Earth & Environment, 1(1): 55.
[18]	EYRE B D, ANDERSSON A J, CYRONAK T, 2014. Benthic coral reef calcium carbonate dissolution in an acidifying ocean[J]. Nature Climate Change, 4(11): 969-976. doi: 10.1038/nclimate2380
[19]	FINDLAY H S, WOOD H L, KENDALL M A, et al, 2011. Comparing the impact of high CO₂on calcium carbonate structures in different marine organisms[J]. Marine Biology Research, 7(6): 565-575. doi: 10.1080/17451000.2010.547200
[20]	FOSTER T, GILMOUR J P, 2016. Seeing red: coral larvae are attracted to healthy-looking reefs[J]. Marine Ecology Progress Series, 559: 65-71. doi: 10.3354/meps11902
[21]	GAO KUNSHAN, ZHENG YANGQIAO, 2010. Combined effects of ocean acidification and solar UV radiation on photosynthesis, growth, pigmentation and calcification of the coralline alga Corallina sessilis (Rhodophyta)[J]. Global Change Biology, 16(8): 2388-2398. doi: 10.1111/gcb.2010.16.issue-8
[22]	GOREAU T J F, HAYES R L, 2021. Global warming triggers coral reef bleaching tipping point: this article belongs to Ambio's 50th anniversary collection. Theme: climate change impacts[J]. Ambio, 50(6): 1137-1140. doi: 10.1007/s13280-021-01512-2
[23]	HOFMANN L C, YILDIZ G, HANELT D, et al, 2012. Physiological responses of the calcifying rhodophyte, Corallina officinalis (L.), to future CO₂ levels[J]. Marine Biology, 159(4): 783-792. doi: 10.1007/s00227-011-1854-9
[24]	HUGGETT M J, MCMAHON K, BERNASCONI R, 2018. Future warming and acidification result in multiple ecological impacts to a temperate coralline alga[J]. Environmental Microbiology, 20(8): 2769-2782. doi: 10.1111/1462-2920.14113 pmid: 29575500
[25]	IPCC, 2014. Climate change 2013: the physical science basis: working group I contribution to the fifth assessment report of the Intergovernmental Panel on Climate Change[M]. Cambridge: Cambridge University Press.
[26]	JIANG LEI, SUN YOUFANG, ZHANG YUYANG, et al, 2017. Impact of diurnal temperature fluctuations on larval settlement and growth of the reef coral Pocillopora damicornis[J]. Biogeosciences, 14(24): 5741-5752. doi: 10.5194/bg-14-5741-2017
[27]	JOHNSON M D, CARPENTER R C, 2012. Ocean acidification and warming decrease calcification in the crustose coralline alga Hydrolithon onkodes and increase susceptibility to grazing[J]. Journal of Experimental Marine Biology and Ecology, 434-435: 94-101.
[28]	JOHNSON M D, MORIARTY V W, CARPENTER R C, 2014. Acclimatization of the crustose coralline alga Porolithon onkodes to variable pCO₂[J]. PLoS One, 9(2): e87678. doi: 10.1371/journal.pone.0087678
[29]	JOHNSON M D, COMEAU S, LANTZ C A, et al, 2017. Complex and interactive effects of ocean acidification and temperature on epilithic and endolithic coral-reef turf algal assemblages[J]. Coral Reefs, 36(4): 1059-1070. doi: 10.1007/s00338-017-1597-2
[30]	JOHNSON M D, CARPENTER R C, 2018. Nitrogen enrichment offsets direct negative effects of ocean acidification on a reef-building crustose coralline alga[J]. Biology Letters, 14(7): 20180371. doi: 10.1098/rsbl.2018.0371
[31]	KAMENOS N A, 2010. North Atlantic summers have warmed more than winters since 1353, and the response of marine zooplankton[J]. Proceedings of the National Academy of Sciences of the United States of America, 107(52): 22442-22447. doi: 10.1073/pnas.1006141107 pmid: 21148422
[32]	KAMENOS N A, BURDETT H L, ALOISIO E, et al, 2013. Coralline algal structure is more sensitive to rate, rather than the magnitude, of ocean acidification[J]. Global Change Biology, 19(12): 3621-3628. doi: 10.1111/gcb.12351 pmid: 23943376
[33]	KIM J H, KIM N, MOON H, et al, 2020. Global warming offsets the ecophysiological stress of ocean acidification on temperate crustose coralline algae[J]. Marine Pollution Bulletin, 157: 111324. doi: 10.1016/j.marpolbul.2020.111324
[34]	LATHAM H, 2008. Temperature stress-induced bleaching of the coralline alga Corallina officinalis: a role for the enzyme bromoperoxidase[J]. Bioscience Horizons: The International Journal of Student Research, 2(1): 104-113.
[35]	LEI XINMING, JIANG LEI, ZHANG YUYANG, et al, 2020. Response of coralline algae Porolithon onkodes to elevated seawater temperature and reduced pH[J]. Acta Oceanologica Sinica, 39(2): 132-137. doi: 10.1007/s13131-020-1548-6
[36]	LICHTENTHALER H K, 1987. Chlorophylls and carotenoids pigments of photosynthetic biomembranes.[J]. Methods in Enzymology, 148: 350-382.
[37]	MANNING J C, CARPENTER R C, MIRANDA E A, 2019. Ocean acidification reduces net calcification and wound healing in the tropical crustose coralline alga, Porolithon onkodes (Corallinales, Rhodophyta)[J]. Journal of Experimental Marine Biology and Ecology, 520: 151225. doi: 10.1016/j.jembe.2019.151225
[38]	MARTIN S, GATTUSO J P, 2009. Response of Mediterranean coralline algae to ocean acidification and elevated temperature[J]. Global Change Biology, 15(8): 2089-2100. doi: 10.1111/gcb.2009.15.issue-8
[39]	MARTIN S, COHU S, VIGNOT C, et al, 2013. One-year experiment on the physiological response of the Mediterranean crustose coralline alga, Lithophyllum cabiochae, to elevated pCO₂ and temperature[J]. Ecology and Evolution, 3(3): 676-693. doi: 10.1002/ece3.2013.3.issue-3
[40]	MASSON-DELMOTTE V, ZHAI P, PIRANI A, et al, 2021. Climate Change 2021: contribution of working group I to the sixth assessment report of the intergovernmental panel on climate change[M]. Cambridge: Cambridge University Press.
[41]	MCCORMACK S, 2014. Ocean acidification, rather than elevated temperature, causes reduced growth and calcification in the geniculate coralline algae, Amphiroa anceps[D]. New South Wales: University of New South Wales: 1-57.
[42]	MCCOY S J, RAGAZZOLA F, 2014. Skeletal trade-offs in coralline algae in response to ocean acidification[J]. Nature Climate Change, 4(8): 719-723. doi: 10.1038/nclimate2273
[43]	MCNICHOLL C, KOCH M S, SWARZENSKI P W, et al, 2020. Ocean acidification effects on calcification and dissolution in tropical reef macroalgae[J]. Coral Reefs, 39(6): 1635-1647. doi: 10.1007/s00338-020-01991-x
[44]	MUÑOZ P T, SÁEZ C A, MARTÍNEZ-CALLEJAS M B, 2018. Short-term interactive effects of increased temperatures and acidification on the calcifying macroalgae Lithothamnion crispatum and Sonderophycus capensis[J]. Aquatic Botany, 148: 46-52. doi: 10.1016/j.aquabot.2018.04.008
[45]	QUI-MINET Z N, COUDRET J, DAVOULT D, et al, 2019. Combined effects of global climate change and nutrient enrichment on the physiology of three temperate maerl species[J]. Ecology and Evolution, 9(24): 13787-13807. doi: 10.1002/ece3.v9.24
[46]	SCHUBERT N, SALAZAR V W, RICH W A, et al, 2019. Rhodolith primary and carbonate production in a changing ocean: the interplay of warming and nutrients[J]. Science of the Total Environment, 676: 455-468. doi: 10.1016/j.scitotenv.2019.04.280
[47]	SINUTOK S, HILL R, DOBLIN M A, et al, 2012. Microenvironmental changes support evidence of photosynthesis and calcification inhibition in Halimeda under ocean acidification and warming[J]. Coral Reefs, 31(4): 1201-1213. doi: 10.1007/s00338-012-0952-6
[48]	SISSINI M N, KOERICH G, DE BARROS-BARRETO M B, et al, 2022. Diversity, distribution, and environmental drivers of coralline red algae: the major reef builders in the Southwestern Atlantic[J]. Coral Reefs, 41(3): 711-725, doi: 10.1007/s00338-021-02171-1. doi: 10.1007/s00338-021-02171-1
[49]	SORDO L, SANTOS R, BARROTE I, et al, 2018. High CO₂ decreases the long-term resilience of the free-living coralline algae Phymatolithon lusitanicum[J]. Ecology and Evolution, 8(10): 4781-4792. doi: 10.1002/ece3.2018.8.issue-10
[50]	SORDO L, SANTOS R, BARROTE I, et al, 2019. Temperature amplifies the effect of high CO₂ on the photosynthesis, respiration, and calcification of the coralline algae Phymatolithon lusitanicum[J]. Ecology and Evolution, 9(19): 11000-11009. doi: 10.1002/ece3.v9.19
[51]	TAMBUTTÉ E, VENN A A, HOLCOMB M, et al, 2015. Morphological plasticity of the coral skeleton under CO₂-driven seawater acidification[J]. Nature Communications, 6: 7368. doi: 10.1038/ncomms8368 pmid: 26067341
[52]	TANAKA Y, SUZUKI A, SAKAI K, 2017. Effects of elevated seawater temperature and phosphate enrichment on the crustose coralline alga Porolithon onkodes (Rhodophyta)[J]. Phycological Research, 65(1): 51-57. doi: 10.1111/pre.2017.65.issue-1
[53]	WEBSTER N S, UTHICKE S, BOTTÉ E S, et al, 2013. Ocean acidification reduces induction of coral settlement by crustose coralline algae[J]. Global Change Biology, 19(1): 303-315. doi: 10.1111/gcb.12008 pmid: 23504741
[54]	WEISS A, MARTINDALE R C, 2017. Crustose coralline algae increased framework and diversity on ancient coral reefs[J]. PLoS One, 12(8): e0181637. doi: 10.1371/journal.pone.0181637
[55]	WILLIAMS B, CHAN P T W, WESTFIELD I T, et al, 2021. Ocean acidification reduces skeletal density of hardground-forming high-latitude crustose coralline algae[J]. Geophysical Research Letters, 48(5): e2020GL091499, doi: 10.1029/2020GL091499. doi: 10.1029/2020GL091499
[56]	YANG FANGFANG, MO JIAHAO, WEI ZHANGLIANG, et al, 2021a. Calcified macroalgae and their bacterial community in relation to larval settlement and metamorphosis of reef-building coral Pocillopora damicornis[J]. FEMS Microbiology Ecology, 97(1): fiaa215. doi: 10.1093/femsec/fiaa215
[57]	YANG FANGFANG, WEI ZHANGLIANG, LONG LIJUAN. 2021b. Transcriptomic and physiological responses of the tropical reef calcified macroalga Amphiroa fragilissima to elevated temperature[J]. Journal of Phycology, 57(4): 1254-1265. doi: 10.1111/jpy.v57.4

指标		自由度	均方	F值	P值 (Tukey HSD)
相对生长速率	Tem	2	0.58	147.46	<0.001 (P_27℃&30℃=0.56; P_27℃&32℃<0.001; P_30℃&32℃<0.001)
	pCO₂	2	0.05	11.40	<0.001(P_L&M=0.01; P_L&H<0.001; P_M&H=0.63)
	Tem & pCO₂	4	0.01	2.64	0.07
叶绿素a含量	Tem	2	197.83	59.12	<0.001(P_27℃&30℃<0.001; P_27℃&32℃<0.001; P_30℃&32℃=0.21)
	pCO₂	2	19.59	5.85	<0.001(P_L&M=0.82; P_L&H=0.01; P_M&H=0.04)
	Tem & pCO₂	4	12.17	3.64	0.03
类胡萝卜素含量	Tem	2	4.60	2.86	0.08
	pCO₂	2	3.65	2.27	0.13
	Tem & pCO₂	4	4.62	2.88	0.05

指标		自由度	均方	F值	P值 (Tukey HSD)
净钙化速率	Tem	2	126762.19	218.46	<0.001(P_27℃&30℃=0.99; P_27℃&32℃<0.001; P_{30℃& 32℃}<0.001)
	pCO₂	2	10572.41	18.22	<0.001(P_L&M=0.79; P_L&H<0.001; P_M&H<0.001)
	Tem & pCO₂	4	8770.87	15.12	<0.001
净溶解速率	Tem	2	12152.63	133.72	<0.001(P_27℃&30℃<0.001; P_27℃&32℃=0.02; P_30℃&32℃<0.001)
	pCO₂	2	11280.92	124.13	<0.001(P_L&M<0.001; P_L&H<0.001; P_M&H=0.003)
	Tem & pCO₂	4	3102.12	34.13	<0.001

The effects of ocean acidification and warming on the growth and calcification in coralline alga Porolithon cf. onkodes

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