珠江口异养细菌时空分布特征及其调控机制

doi:10.11978/2017042

Abstract

Abstract:

Estuaries are important land-sea interaction zones with unique biogeochemical processes and are important places to study the carbon cycle. Little is known on bacterial regulation in carbon cycling in the Pearl River Estuary. To examine the relative contribution of different groups of heterotrophic bacteria (particle-attached bacteria vs free-living bacteria, and high nucleic acid bacteria vs low nucleic acid bacteria), spatial and temporal variation in heterotrophic bacterial abundance was investigated during three contrasting periods (May 2015, August 2015 and January 2016) in the Pearl River Estuary. The total bacterial abundance was the highest in spring (an average of 2.94±1.30×10⁹ cells •L^-1 at the surface and 2.81±1.50×10⁹ cells •L^-1 at the bottom), moderate in summer (2.32±0.43×10⁹ cells •L^-1 at the surface and 1.85±0.50×10⁹cells •L^-1 at the bottom), and the lowest in winter (1.06±0.33×10⁹ cells •L^-1 at the surface and 9.76±3.44×10⁸ cells •L^-1 at the bottom). Bacterial abundance at the surface was slightly higher than that at the bottom. Bacterial abundance decreased spatially from the upstream to the downstream of the estuary, suggesting that sewage input contributed to high bacterial abundance in the upstream, and that the downstream decrease resulted from the mixing of river discharge and seawater. The peaks of bacterial abundance and Chl a were observed at the frontal zone in the wet season, since a front increased the residence time of nutrients and plankton, and favored plankton growth. According to the spatial variation trend of bacterial abundance, it could be inferred that the heterotrophic bacteria of the estuary mainly came from upstream inputs. Heterotrophic bacterial communities were dominated by particle-attached bacteria, which accounted for 78.65%, 70.32% and 68.17% of the total bacterial abundance in spring, summer and winter, respectively. The metabolically active bacteria with high nucleic acid dominated in the particle-attached bacteria, while the low nucleic acid group was the dominant component of the free-living bacteria.

Key words: Pearl River Estuary, heterotrophic bacteria, particle-attached bacteria, free-living bacteria, correlation

CLC Number:

P735.12

Xiangfu LI, Jie XU, Zhen SHI, Ruihuan LI. Spatial and temporal variation in heterotrophic bacteria and their regulators in the Pearl River Estuary[J].Journal of Tropical Oceanography, 2018, 37(1): 27-36.

Figures/Tables 6

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Tab. 1

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References 44

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		温度	盐度	SPM	NO^- 3	NO^-2	NH+ 4	PO3- 4	SiO2- 4	DOC	Chl a
201505	TBA^#		-0.67*	0.71*		0.76**	0.78*	0.76*	0.73*		0.69*
	THNA%		-0.94**		0.94**	0.84**		0.94**	0.93**	0.59*
	FBA	0.56*
	FHNA%
	PBA^#		-0.70*	0.78**		0.71*	0.72**	0.64*	0.75**		0.66*
	PHNA%		-0.82**							0.60*
	PBA%		-0.84**	0.65*	0.68*	0.656*		0.69**	0.76**
201508	TBA	0.68**	-0.50*						0.52*
	THNA%										0.56*
	FBA	0.54*	-0.50*		0.51*				0.57*
	FHNA%
	PBA
	PHNA%	0.72**	-0.72**		0.67*				0.67*
	PBA%
201601	TBA	-0.63**	-0.56*		0.54*	0.66**	0.54*
	THNA%	-0.83**	-0.86**		0.84**	0.58*	0.64*	0.77**	0.82**
	FBA	-0.83**	-0.76**		0.75*	0.57*	0.56*	0.52*	0.56*
	FHNA%
	PBA	-0.53*	-0.52*		0.51*	0.68**
	PHNA%	-0.75**	-0.76**		0.76**	0.58*		0.73**	0.69*
	PBA%	-0.68**	-0.63**		0.59*				0.53*

航次时间	线性回归方程
201505	TBA=1.50[±0.18]×10⁹+ 1.52[±0.29]×10⁸Chl a	r²=0.800, p<0.01
201505	PBA=8.88[±2.14]×10⁸ + 1.33[±0.33]×10⁸Chl a	r²=0.693, p<0.01
201508	PBA=1.04[±0.11]×10⁹+ 1.02[±0.28]×10⁸Chl a	r²=0.821, p<0.05
201601	TBA=7.46[±1.97]×10⁷+ 9.20[±3.15]×10⁷Chl a + 7.25[±1.32]×10⁷NO^- 2	r²=0.732, p<0.01
	FBA=3.55[±0.60]×10⁹- 1.79[±0.32]×10⁸T	r²=0.739, p<0.01
	PBA=3.85[±1.29]×10⁸ - 4.49[±1.21]×10⁷ NO^- 2	r²=0.534, p<0.01

Spatial and temporal variation in heterotrophic bacteria and their regulators in the Pearl River Estuary

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