Journal of Tropical Oceanography ›› 2018, Vol. 37 ›› Issue (2): 55-62.doi: 10.11978/2017049CSTR: 32234.14.2017049

• Orginal Article • Previous Articles     Next Articles

Analysis of physicochemical properties and structure characteristics of several crude algal polysaccharides

Ping HOU1(), Jun MA2,3, Yan CHEN2,3, Zhisheng PEI3, Jing ZHAO3, Xipeng YE3, Fangyan JIANG2,3, Hai HUANG2,3()   

  1. 1. Sanya Aviation and Tourism College, Sanya 572000, China
    2. Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources, Sanya 572022, China
    3. College of Life Science and Ecology, Hainan Tropical Ocean University, Sanya 572022, China
  • Received:2017-04-26 Revised:2017-07-18 Online:2018-04-10 Published:2018-04-11
  • Supported by:
    Hainan Province Natural Science Foundation (20153162, 20163071, 20163074)

Abstract:

The monosaccharide composition, functional groups and molecular weight of five crude algal polysaccharides were analyzed by using fourier transform infrared spectroscopy (FTIR), gas chromatography (GC) and gel permeation chromatography (GPC). The results showed that five crude algal polysaccharides contained both xylose and fucose. Padina australis polysaccharide, which was the high molecular weight uniformity-polysaccharide, contained the rich sorbitol, whereas Ulva lactuca polysaccharide had a wide molecular weight distribution and was the poor uniformity-polysaccharide. The analysis of FTIR indicated that five crude polysaccharides were both acidic polysaccharides. Caulerpa sertularioides, Sargassum polycystum and Betaphycus gelatinum polysaccharide had obvious absorption peak of sulfate group. The structure of Betaphycus gelatinum polysaccharide included furanose and pyranose, and the glycosidic bond might include beta and alpha types. The sulfate content of Padina australis polysaccharide was 38.43% (±2.38%), which was significantly higher than that of the other kinds of algal polysaccharides, whereas the sulfate content of Caulerpa sertularioides polysaccharide was only 8.92%(±1.04%).

Key words: algal polysaccharide, component, structure, molecular weight, sulfate group