饲料中添加芽孢杆菌和中草药制剂对凡纳滨对虾生长及肠道菌群的影响

doi:10.11978/j.issn.1009-5470.2010.04.132

Abstract

Abstract:

A feeding experiment was conducted to investigate the effects of Bacillus and traditional Chinese medicines (TCM) on growth and intestinal bacterial flora of shrimp L. vannamei. Four experimental diets were formulated by adding the Bacillus and TCM to basil diet (C): 0.2% TCM (M), 0.20% TCM+0.30% Bacillus (BM1), 0.10% TCM+0.15% Bacillus (BM2), 0.30% Bacillus (B). The concentration of Bacillus was 109 CFU·g-1. The TCM consisted of a mixture of herbs and plant materials Isatis tinctoria L., Isatis indigodica Fort, Forsythia suspersa Vahl, Corydalis bungeana Turez, Pogostemon cablin (blanco) Benth and Astragalus menbranaceus (Fisch.) Bge. Acclimatized shrimp with an average initial weight of 1.91±0.03 g were selected and stocked into 15 cement tanks at a stocking density of 80 shrimp. Each aquarium contained 1500L of sand filtered seawater with a flow rate of 0.9L·min-1. The water was continuously aerated with two air stones. Each diet was given to shrimp four times (07:00, 12:00, 17:00, 23:00) a day to saturation for a period of 56 days. During the experiment period, the water temperature fluctuated between 19.6 and 25.2ºC, salinity between 30 and 31 psu. At the end of feeding trial, survival ranged from 95.83% to 98.33% with no significant difference (P>0.05) among all groups. Growth measured as final weight was slightly higher (P>0.05) in shrimp fed with BM1, M and B than that with C, and there was a significant difference (P<0.05) between BM2 and C. In case of weight gain (WG) and specific growth rate (SGR), the shrimp fed with M, BM1 and BM2 exhibited significantly (P<0.05) higher values than that with C and B. Of the five diets, BM2 exhibited the best performance, followed by BM1. The combination of Bacillus spp. and TCM, therefore, proved to be more effective and economically viable. The total bacteria counts and Vibrio counts in shrimp fed with M were the most; the counts with shrimp fed with BM1 and BM2 were the least. In each group 30 bacterial strains were purified and identified. These bacterial strains belonged to 12, 8, 6, 5, 7 genera in C, M, BM1, BM2 and B, respectively. Most of the bacteria were gram negative bacteria; they counted a percentage of 86% to 93% in each group. Vibrio spp., Photobacterium spp. and Pseudomonas spp. were the predominant bacterial flora; however, their values varied in different groups. The results showed that the diets supplemented with TCM and Bacillus also changed the number and composition of intestinal bacterial flora.

Key words: Bacillus spp., traditional Chinese medicines, Litopenaeus vannamei, growth, bacteria

YU Ming-chao,LI Zhuo-jia,LIN Hei-zhao,YANG Ying-ying. Effects of dietary Bacillus spp. and traditional Chinese medicines on growth and intestinal bacterial flora of shrimp Litopenaeus vannamei[J].Journal of Tropical Oceanography, 2010, 29(4): 132-137.

References

[1] 许兵, 纪伟尚, 张鹏等. 对虾病原菌抑菌药物的研究[J]. 青岛海洋大学学报, 1993, 23(2): 43-51.
[2] BOYD C E, MASSAAUT L. Risks associated with the use of chemical in pond aquaculture [J]. Aqua Engin, 1999, 20(2): 113–132.
[3] GATESOUPE F J. The use of probiotics in aquaculture [J]. Aquaculture, 1999, 180: 147–165.
[4] JIAN J C, WU Z H. Effects of Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosiaena crocea (Richardson) [J]. Aquaculture, 2003, 218: 1–9.
[5] THOMPSON F L, ABREU P C, CAVALLI R. The use of microorganisms as food source for Penaeus paulensis larvae [J]. Aquaculture, 1999, 174: 139–153.
[6] VERSCHUERE L, ROMBAUT G, SORGELOOS P, et al. Probiotic bacteria as biological control agents in aquaculture [J]. Microbiol Mol Biol Rev, 2000, 64: 655–671.
[7] LIN H Z, GUO Z X, YANG Y Y, et al. Effect of dietary probiotics on apparent digestibility coefficients of nutrients of white shrimp Litopenaeus vannamei Boone [J]. Aquac Res, 2004, 35: 1441–1447.
[8] JIAN J C, WU Z H. Influences of traditional Chinese medicine on non-specific immunity of Jian common carp (Cyprinus carpio var. Jian) [J]. Fish Shellfish Immunol, 2004, 16: 185–191.
[9] 罗日祥. 中药制剂对中国对虾免疫活性物的诱导作用[J]. 海洋与湖沼, 1997, 28(6): 573–578.
[10] CHANSUE N, PONPORNPISIT A, ENDO M, et al. Improved immunity of tilapia Oreochromis niloticus by C-UP III, a herb medicine [J]. Fish Pathol, 2000, 35: 89–90.
[11] LIN H Z, LI Z J, CHEN Y Q, et al. Effect of dietary traditional Chinese medicines on apparent digestibility coefficients of nutrients for white shrimp Litopenaeus vannamei, Boone [J]. Aquaculture, 2006, 253: 495–501.
[12] OLIVER J D. Taxonomic scheme for the identification of marine bacteria [J]. Doco Sea Res, 1982, 29 (6): 795–798.
[13] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 353–372.
[14] 丁贤, 李卓佳, 陈永青, 等. 芽孢杆菌对凡纳滨对虾生长和消化酶活性的影响[J]. 中国水产科学, 2004, (11): 17–21.
[15] 李卓佳, 曹煜成, 陈永青, 等. 地衣芽孢杆菌De株的胞外产物对凡纳滨对虾脂肪酶活性影响的体外实验[J]. 高技术通讯, 2006, 16(2): 191–195.
[16] RENGPIPAT S, PHIANPHAK W, PIYATIRATITIVORAKUL S, et al. Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth [J]. Aquaculture, 1998, 167: 301– 313.
[17] BOMBA A, NEMCOÐ R, GANCARCIKOVA S, et al. Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids [J]. Brit J Nutr, 2002, 88 (Suppl. 1): 95–99.
[18] 王吉桥, 孙永新, 张剑诚. 金银花等复方草药对牙鲆生长、消化和免疫能力的影响[J]. 水产学报, 2006, 30(1): 90–96.
[19] 邱小琮, 周洪琪, 刘小刚, 等. 中草药添加剂对异育银鲫生长和蛋白质消化吸收的影响[J]. 水产学报, 2002, 26(6): 551–555.
[20] 宛立, 王吉桥, 高峰, 等. 南美白对虾肠道细菌菌群分析[J]. 水产科学, 2006, 25(1): 13–15.
[21] 李卓佳, 林亮, 杨莺莺, 等. 芽孢杆菌制剂对凡纳滨对虾Litopenaeus vannamei肠道微生物群落的影响[J]. 南方水产, 2005, 1(3): 54–59.
[22] 刘红柏, 张颖, 杨雨辉, 等. 5种中草药作为饲料添加剂对鲤肠内细菌及生长的影响[J]. 大连水产学院学报, 2004(1): 16–20.
[23] 李莉, 陈孝煊, 蔡雪峰. 穿心莲对草鱼肠内细菌的影响[J]. 内陆水产, 2002 (8): 35–37.
[24] 徐怀恕, 杨学宋, 李筠. 对虾苗期细菌病害的诊断与控制[M]. 北京: 海洋出版社, 1999: 136–145.
[25] AGUIRRE GUZMAN G, VAZQUEZ JUAREZ R, ASCENCIO F. Differences in the susceptibility of American white shrimp larval substages (Litopenaeus vannamei) to four Vibrio species [J]. Invertebr Pathol, 2001, 78 (4): 215–219.
[26] 李筠, 吕艳, 李军, 等. 苗期中国对虾幼体异养细菌区系及其变化与病害发生的关系[J]. 中国海洋大学学报, 2004, 34(6), 1003–1007.
[27] 许兵, 周新婷, 徐怀恕, 等. 几种中草药对光合细菌生长的促进作用[J]. 青岛海洋大学学报, 1994, 24(1): 47–51.
[28] 丁轲. 益生乳酸杆菌的筛选及中草药协同作用的研究 [D]. 雅安: 四川农业大学预防兽医学, 2003.
[29] 刘明杰, 林琳, 王钊. 肠道细菌对天然药物代谢的研究进展[J]. 中国现代应用药学杂志, 2001, 18(2): 90–91.

Effects of dietary Bacillus spp. and traditional Chinese medicines on growth and intestinal bacterial flora of shrimp Litopenaeus vannamei

PDF (PC)

Like

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	AN Fan, JIANG Yue, WANG Yu, CAO Guangping, GAO Chenghai, LIU Yonghong, YI Xiangxi, BAI Meng. Studies on secondary metabolites of endophytic fungus Aspergillus terreus GXIMD 03158 isolated from mangroves Acanthus ilicifolius L. [J]. Journal of Tropical Oceanography, 2024, 43(5): 41-48.
[2]	WANG Jiaxi, LU Humu, QI Xin, GAO Chenghai, LIU Yonghong, LUO Xiaowei. Study on the secondary metabolites from the Weizhou Island coral Acropora austera associated fungus Arachniotus ruber GXIMD 02510 and their antibacterial activity [J]. Journal of Tropical Oceanography, 2024, 43(4): 174-180.
[3]	SUN Manman, ZENG Yanbo, XU Han, YAO Ligong, GUO Yuewei, SU Mingzhi. Chemical composition and antibacterial activities of the soft coral Lobophytum sp. from the South China Sea [J]. Journal of Tropical Oceanography, 2024, 43(4): 189-197.
[4]	LIU Didi, ZHANG Xiyang, SUN Fulin, WANG Mingzhuang, TAN Fei, SHI Qi, WANG Guan, YANG Hongqiang. Microbial communities and specific strains within beachrocks of the South China Sea: implications for the origin of beachrock* [J]. Journal of Tropical Oceanography, 2024, 43(4): 112-122.
[5]	ZHOU Weihua, LI Yingxin, GUO Yajuan, HUO Jiaxin, SONG Yan, ZHU Qing, YUAN Xiangcheng, LIU Sheng, HUANG Hui. Effects of ocean acidification and warming on the growth and calcification of two reef-building corals* [J]. Journal of Tropical Oceanography, 2024, 43(3): 49-57.
[6]	LIN Xianzhi, ZHOU Yanyan, LIN Haoye, HU Simin, HUANG Hui, ZHANG Li, LIU Sheng. Diet analysis of the parrotfish (Scarus globiceps) in coral reefs of the Nansha Islands [J]. Journal of Tropical Oceanography, 2024, 43(3): 100-108.
[7]	LIU Ying, XIAO Yang, ZHU Zhenxin, LIU Hongcun, JIANG Mingguo, ZHU Yuzhang, LIN Kun, WU Jincheng, LU Xiaomei, HUANG Xiaoning, LIANG Haina, LU Wensen, YANG Lifang. Study on the Secondary Metabolites of Marine Streptomyces Sporoverrucosus 33510 [J]. Journal of Tropical Oceanography, 2024, 43(2): 128-134.
[8]	FENG Ting, SUN Jian, WANG Yufei, PAN Weibin, QIN Xucan, QIN Bingyun, ZHOU Liman, WANG Cong, WANG Pei, KONG Fandong. Study on the chemical constituents and pharmacological activity of the marine-derived fungus Aspergillus fumigatus DL-p0m-g2 in the Beibu Gulf [J]. Journal of Tropical Oceanography, 2024, 43(1): 154-166.
[9]	HUANG Yu, WANG Lin, MAI Zhimao, LI Jie, ZHANG Si. Isolation and characterization of sand fixation ability of bacteria in biological soil crusts of the tropical islands, South China Sea [J]. Journal of Tropical Oceanography, 2023, 42(6): 101-110.
[10]	ZENG Buyan, LIANG Zhifeng, LUO Qinqin, ZENG Ling, YANG Changgeng, WANG Liyun, SUN Yulin. Molecular identification, secondary metabolites and biological activities of a deep-sea-derived fungus 101#^* [J]. Journal of Tropical Oceanography, 2023, 42(5): 104-114.
[11]	YUAN Huabiao, HUANG Jingtong, WAN Peng, CAI Bingna, PAN Jianyu, ZHANG Yuhang, LING Juan, CHEN Hua. Screening and efficacy evaluation of antibacterial peptides from Holothuroidea [J]. Journal of Tropical Oceanography, 2023, 42(4): 184-194.
[12]	SONG Yongxiang, LI Xiaoyue, JU Jianhua. The isolation and identification of neoabyssomicin H from the deep-sea derived Streptomyces koyangensis SCSIO 5802^* [J]. Journal of Tropical Oceanography, 2023, 42(3): 169-173.
[13]	LU Tianmei, GUAN jiasong, QIN Shijing, LIU Yonghong, SU Zhiwei. Preliminary study on the culturable myxobacteria resources from the Beibu Gulf, Guangxi and their antibacterial activity [J]. Journal of Tropical Oceanography, 2023, 42(3): 158-168.
[14]	WANG Pengxia, ZHAO Yi, DU Xiaofei, WANG Weiquan, WANG Xiaoxue. Identification and functional study of the genomic island GIPspSM9913 in Pseudoalteromonas sp. SM9913 [J]. Journal of Tropical Oceanography, 2023, 42(2): 45-53.
[15]	YANG Fangfang, XIAO Zhiliang, WEI Zhangliang, HUANG Yi, LONG Lijuan. The effects of ocean acidification and warming on the growth and calcification in coralline alga Porolithon cf. onkodes [J]. Journal of Tropical Oceanography, 2023, 42(1): 87-97.