磷酸根对海洋克雷伯氏菌合成1,3-丙二醇代谢的影响

doi:10.11978/2014136

Abstract

Abstract: Klebsiella pneumoniae HSL4 is an excellent strain for the fermentation of 1,3-propanediol (1,3-PDO), which was isolated from coastal mangrove sediment environment. Many nutrient factors are involved in the culture medium of K. pneumoniae HSL4. Among them, phosphate is one of the main factors, which have a significant impact on the fermentation. The effects of the low concentration of phosphate (LP: 1.6×10^-4mol·L^-1 PO₄^3-) and the high concentration phosphate (HP: 5.0×10^-3mol·L^-1 PO₄^3-) on cell growth, 1,3-PDO and by-product (acetic succinic acid, lactic acid, acid, 2,3-butanediol, and ethanol) production were studied. The impacts of phosphate on the metabolism of K. pneumoniae HSL4 were characterized by metabolic flux analysis. The results showed that the final concentration of 1,3-PDO was 75g·L^-1 under both LP and HP in the culture medium. Significantly, the 1,3-PDO conversion was higher, but the substrate glycerol consumption and by-products (except for 2,3-butanediol) accumulation were less under LP condition compared to HP condition. The productions of acetic acid and ethanol were higher under HP condition. The accumulations of acetic acid and ethanol will change the environment of fermentation and increase the difficulty of product separation in the downstream. Metabolic flux distributions in four fermentation periods (adjustmental phase, log phase, stationary phase, and decline phase) were analyzed. This study will help researchers to choose reasonable phosphate concentration in the culture broth and provide information for process control and metabolic regulation in 1,3-PDO fermentation by K. pneumoniae HSL4.

Key words: Sciences, Guangzhou 510301, China, 2. University of Chinese Academy of Sciences, Beijing 100049, China, 3. College of Marine Science, Hainan University, Haikou 570228, China

LI Li-li, ZHOU Sheng, GAO-Pin, GUO Chuan-yu, YU Ye-ping, NI Song-wei, LI Peng-fei, QIN Qi-wei. Effect of phosphate on 1,3-propanediol metabolism by marine Klebsiella pneumonia HSL4[J].Journal of Tropical Oceanography, 2015, 34(5): 27-35.

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Effect of phosphate on 1,3-propanediol metabolism by marine Klebsiella pneumonia HSL4

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