基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成*

doi:10.11978/2019105

Abstract

Abstract:

Uroteuthis chinensis and Sthenoteuthis oualaniensis are key species of the cephalopods in the South China Sea, which have important effects on the material flow and energy circulation in the marine ecosystem. The study of feeding ecology of these two cephalopods will be great significant to the construction of marine food web. Because of the sharp tooth decay, the stomach contents are all scorpion-like, and it is difficult to accurately identify them by traditional morphological methods. And the effectiveness of molecular techniques in feeding analysis of these two species has not been fully validated. In this study, we identified the species (or genus) in the stomach contents of U. chinensis and S. oualaniensis based on the mitochondrial COI gene, and used the Generalized Mixed Yule Coalescent (GMYC) model for species definition and phylogenetic tree building. The results showed that of a total of 41 stomach contents sequences (28 from U. chinensis and 13 from S. oualaniensis), 30 (73%) were identified as species, and the rest were determined as genus (all are crustaceans). The diets of U. chinensis in Shantou-Taiwan Shoal fishing ground included fish, cephalopods, and crustaceans; and the diets of S. oualaniensis in the Central South China Sea were fish and cephalopods. Cannibalism occurred in both species, but was more pronounced in S. oualaniensis.

Key words: Uroteuthis chinensis, Sthenoteuthis oualaniensis, diet, COI, DNA barcoding, South China Sea, Shantou-Taiwan Shoal fishing ground

CLC Number:

P735

Mengna LIU, Lei XU, Xuehui WANG, Yu LIU, Miaodi WANG, Yongsong QIU, Jiangfeng ZHU, Yinglin HE, Weilie BEI, Feiyan DU. Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence[J].Journal of Tropical Oceanography, 2020, 39(4): 61-69.

Figures/Tables 7

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References 34

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	站位	东经	北纬
鸢乌贼	S3	111°37'50"	14°58'04"
	S9	110°35'06"	14°02'10"
	S16	117°28'28"	13°00'00"
	S18	110°30'35"	11°02'09"
	S19	111°28'51"	10°59'30"
	S20	113°51'30"	11°02'17"
	S21	114°32'36"	11°06'13"
	S26	110°28'22"	9°30'53"
中国枪乌贼	SQ	118°29'21"	23°05'46"

引物名称	序列(5′-3′)	参考文献
HCO2198(下游)	TAAACTTCAGGGTGACCAAAAAATCA	Folmer et al, 1994
dgHCO2198(下游)	TAAACTTCAGGGTGACCAAARAAYCA	Meyer, 2003
jgHCO2198(下游)	TAIACYTCIGGRTGICCRAARAAYCA	Geller et al, 2013
mlCOIintF(上游)	GGWACWGGWTGAACWGTWTAYCCYCC	Leray et al, 2013

最佳匹配物种	相似度	序列号	样品号
克氏副葉鰺 Alepes Swainson	99.90%	KY371115.1	N0709、N0711、N0752、N0759、N0767、N0771、N0772
蓝圆鲹 Decapterus maruadsi	100.00%	MK264594.1	N0718
金带细鲹 Selaroides leptolepis	99.64%	JX261038.1	N0740、N0742、N0779
三线鯻 Terapon puta	100.00%	KY372209.1	N0722
怀氏方头鲳 Cubiceps whiteleggii	100.00%	KY371367.1	N0743
黄泽小沙丁鱼 Sardinella lemuru	100.00%	MK264430.1	N0758
角突仿对Kishinouyepenaeopsis amicus	98.71%	KR349255.1	N0720、N0723
赤虾属 Metapenaeopsis sp.	87.05%	KR150460.1	N0753
刺铠虾属 Munida sp.	86.58%	AY350938.1	N0749、N0754、N0757、N0761、N0768、N0769、N0775
招潮蟹属 Uca sp.	84.40%	AB813678.1	N0735
团扇蟹属 Ozius sp.	87.45%	HM638053.1	N0770
梭子蟹属Portunus sp.	88.67%	JX398096.1	N0777
中国枪乌贼 Uroteuthis chinensis	100.00%	MG192385.1	N0736

最佳匹配物种	相似度	序列号	样品号
孔锯鳞鱼 Myripristis kuntee	99.53%	MK658063.1	S20-41-2
尾斑棘鳞鱼 Sargocentron caudimaculatum	100.00%	MF409503.1	S19-44-4
克氏副葉鰺 Alepes Swainson	100.00%	MK264421.1	S3-23-3
脂眼凹肩鲹 Selar crumenophthalmus	100.00%	MH638727.1	S26-14-2
鲣鱼 Katsuwonus pelamis	99.78%	MH638766.1	S19-44-3、S16-24-3
金焰灯笼魚 Myctophum aurolaternatum	100.00%	KJ555427.1	S20-28-3
鸢乌贼微型群 Sthenoteuthis oualaniensis	100.00%	MF411130.1	S18-1、S18-14、S19-31-2
鸢乌贼 Sthenoteuthis oualaniensis	100.00%	MF411105.1	S21-45-1、S3-25、S9-22

Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence

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