Tab. 1

Sequences of designed primers used in this study"

Tab. 2

Plasmids transfection in dual luciferase reporter assay"

Fig. 1

Full length of the Crassostrea gigas cGAS open reading frame sequence and deduced amino acid sequence. The zinc finger structure is marked with a blue box and the Mab21 structural domain is marked with a purple box. * indicates protein translation termination, with initiation codon ATG and termination codon TAA in red font"

Fig. 2

Schematic of cGAS structures in different species"

Fig. 3

Multiple alignment of cGAS amino acid sequences between Crassostrea gigas and other species. NTase catalytical sites are marked with red dots, and the zinc-ribbon domain is indicated by a box, with “---” representing an amino acid deletion. “*”, “:” and “.” present identical, highly conserved, and less conserved amino acid residues, respectively"

Fig. 4

Phylogenetic evolutionary tree of cGAS constructed by maximum likelihood method. Red lettering represents Crassostrea gigas"

Fig. 5

Expression levels of CgcGAS in different tissues. All data are expressed as mean±SEM, n =3. Significant differences (P< 0.05) are identified by different letters, a-c"

Fig. 6

Subcellular localization of cGAS in Crassostrea gigas. Shown on the left is the fluorescent fusion protein of pEGFP and cGAS; shown in the middle is the nucleus after DAPI staining; and shown on the right is the combined image. The microscopic observation results of HEK293T cells at 40 magnifications"

Fig. 7

The activating effect of cGAS on NF-κB (a) and ISRE-luc (b) in Crassostrea gigas. All data are expressed as mean±SEM, n = 3. Significant differences (P< 0.05) are identified by different letters, a-c"

Fig. 8

Expression of downstream factors after cGAS knockdown in Crassostrea gigas; (a) fluorescence quantitative PCR was used to detect the knockdown effect; (b) expression of downstream immune factors after cGAS knockdown. All data are presented as mean ± SEM, with significance denoted as * for P<0.05, ** for P<0.01, *** for P<0.001, and **** for P<0.0001"