基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成*

doi:10.11978/2019105

Abstract

Abstract:

Uroteuthis chinensis and Sthenoteuthis oualaniensis are key species of the cephalopods in the South China Sea, which have important effects on the material flow and energy circulation in the marine ecosystem. The study of feeding ecology of these two cephalopods will be great significant to the construction of marine food web. Because of the sharp tooth decay, the stomach contents are all scorpion-like, and it is difficult to accurately identify them by traditional morphological methods. And the effectiveness of molecular techniques in feeding analysis of these two species has not been fully validated. In this study, we identified the species (or genus) in the stomach contents of U. chinensis and S. oualaniensis based on the mitochondrial COI gene, and used the Generalized Mixed Yule Coalescent (GMYC) model for species definition and phylogenetic tree building. The results showed that of a total of 41 stomach contents sequences (28 from U. chinensis and 13 from S. oualaniensis), 30 (73%) were identified as species, and the rest were determined as genus (all are crustaceans). The diets of U. chinensis in Shantou-Taiwan Shoal fishing ground included fish, cephalopods, and crustaceans; and the diets of S. oualaniensis in the Central South China Sea were fish and cephalopods. Cannibalism occurred in both species, but was more pronounced in S. oualaniensis.

Key words: Uroteuthis chinensis, Sthenoteuthis oualaniensis, diet, COI, DNA barcoding, South China Sea, Shantou-Taiwan Shoal fishing ground

CLC Number:

P735

Mengna LIU, Lei XU, Xuehui WANG, Yu LIU, Miaodi WANG, Yongsong QIU, Jiangfeng ZHU, Yinglin HE, Weilie BEI, Feiyan DU. Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence[J].Journal of Tropical Oceanography, 2020, 39(4): 61-69.

Figures/Tables 7

Tab. 1

Tab. 2

Fig. 1

Tab. 3

Fig. 2

Tab. 4

Fig. 3

References 34

[1]	陈大纲, 张美昭, 2015. 中国海洋鱼类[M]. 青岛: 中国海洋大学出版社: 280-1883(in Chinese).
[2]	陈新军, 2004. 渔业资源与渔场学[M]. 北京: 海洋出版社: 51-52(in Chinese).
[3]	陈新军, 刘必林, 王尧耕, 2009. 世界头足类[M]. 北京: 海洋出版社: 312-313(in Chinese).
[4]	戴泉水, 卢振彬, 洪明进, 等, 2004. 台湾海峡南部海域游泳生物区系组成和渔业资源现状[J]. 中国水产科学, 11(4):360-366.
	DAI QUANSHUI, LU ZHENBIN, HONG MINGJIN, et al, 2004. Fauna composition of necton and fishery resources in the southern waters of Taiwan Strait[J]. Journal of Fishery Sciences of China, 11(4):360-366 (in Chinese with English abstract).
[5]	董正之, 1988. 中国动物志, 软体动物门, 头足纲[M]. 北京: 科学出版社: 88-95(in Chinese).
[6]	范江涛, 冯雪, 邱永松, 等, 2013. 南海鸢乌贼生物学研究进展[J]. 广东农业科学, 40(23):122-128.
	FAN JIANGTAO, FENG XUE, QIU YONGSONG, et al, 2013. Review on the biology of purpleback flying squid in South China Sea[J]. Guangdong Agricultural Science, 40(23):122-128 (in Chinese with English abstract).
[7]	龚金科, 1981. 闽南-台湾浅滩渔场台湾枪乌贼的洄游分布和生物学特性的探讨[J]. 福建水产科技, ( 2):15-26 (in Chinese).
[8]	龚玉艳, 詹凤娉, 杨玉滔, 等, 2016. 南海鸢乌贼摄食习性的初步研究[J]. 南方水产科学, 12(4):80-87.
	GONG YUYAN, ZHAN FENGPING, YANG YUTAO, et al, 2016. Feeding habits of Symplectoteuthis oualaniensis in the South China Sea[J]. South China Fisheries Science, 12(4):80-87 (in Chinese with English abstract).
[9]	龚玉艳, 孔啸兰, 杨玉滔, 等, 2018. 南海鸢乌贼微型群体的摄食习性研究[J]. 海洋渔, 40(4):394-403.
	GONG YUYAN, KONG XIAOLAN, YANG YUTAO, et al, 2018. Feeding habits of dwarf-form Sthenoteuthis oualaniensis population in the South China Sea[J]. Marine Fisheries, 40(4):394-403 (in Chinese with English abstract).
[10]	黄美珍, 2004. 台湾海峡及邻近海域4种头足类的食性和营养级研究[J]. 台湾海峡, 23(3):331-340.
	HUANG MEIZHEN, 2004. Study on feeding habits and nutrient level of four cephalopod species from Taiwan Strait and its adjacent areas[J]. Journal of Oceanography in Taiwan Strait, 23(3):331-340 (in Chinese with English abstract).
[11]	李斌, 陈国宝, 郭禹, 等, 2016. 南海中部海域渔业资源时空分布和资源量的水声学评估[J]. 南方水产科学, 12(4):28-37.
	LI BIN, CHEN GUOBAO, GUO YU, et al, 2016. Hydroacoustic assessment of spatial-temporal distribution and biomass of fishery resources in the central South China Sea[J]. South China Fisheries Science, 12(4):28-37 (in Chinese with English abstract).
[12]	唐启升, 1999. 海洋食物网与高营养层次营养动力学研究策略[J]. 海洋水产研究, 20(2):1-6.
	TANG QISHENG, 1999. Strategies of research on marine food web and trophodynamics between high trophic levels[J]. Marine Fisheries Research, 20(2):1-6 (in Chinese with English abstract).
[13]	颜云榕, 冯波, 卢伙胜, 等, 2012. 南沙群岛北部海域鸢乌贼(Sthenoteuthis oualaniensis)夏季渔业生物学研究[J]. 海洋与湖沼, 43(6):1177-1186.
	YAN YUNRONG, FENG BO, LU HUOSHENG, et al, 2012. Fishery biology of purpleback flying squid Sthenoteuthis oualaniensis in northern sea areas around Nansha islands in summer[J]. Oceanologia et Limnologia Sinica, 43(6):1177-1186 (in Chinese with English abstract).
[14]	张宇美, 颜云榕, 卢伙胜, 等, 2013. 西沙群岛海域鸢乌贼摄食与繁殖生物学初步研究[J]. 广东海洋大学学报, 33(3):56-64.
	ZHANG YUMEI, YAN YUNRONG, LU HUOSHENG, et al, 2013. Study on feeding and reproduction biology of purple flying squid, Sthenoteuthis oualaniensis in the western south china sea[J]. Journal of Guangdong Ocean University, 33(3):56-64 (in Chinese with English abstract).
[15]	张壮丽, 叶孙忠, 洪明进, 等, 2008. 闽南—台湾浅滩渔场中国枪乌贼生物学特性研究[J]. 福建水产, 3(1):1-5.
	ZHANG ZHUANGLI, YE SUNZHONG, HONG MINGJIN, et al, 2008. Biological characteristics of the Chinese squid (Loligo chinensis) in Minnan-Taiwan shallow fishing ground[J]. Journal of Fujian Fisheries, 3(1):1-5 (in Chinese with English abstract).
[16]	郑玉水, 杨甘霖, 曾建忠, 等. 台湾海峡枪乌贼资源探捕调查报告[J]. 福建水产. 1988: 8-16.
[17]	ALTSCHUL S F, MADDEN T L, SCHAFFER A A, et al, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs[J]. Nucleic Acids Research, 25(17):3389-3402. pmid: 9254694
[18]	COSTA F O, DEWAARD J R, BOUTILLIER J, et al, 2007. Biological identifications through DNA barcodes: the case of the Crustacea[J]. Canadian Journal of Fisheries and Aquatic Sciences, 64(2):272-295.
[19]	DEAGLE B E, EVESON J P, JARMAN S N, 2006. Quantification of damage in DNA recovered from highly degraded samples--a case study on DNA in faeces[J]. Frontiers in Zoology, 3:11. pmid: 16911807
[20]	DRUMMOND A J, SUCHARD M A, XIE D, et al, 2012. Bayesian Phylogenetics with BEAUti and the BEAST 1.7[J]. Molecular Biology and Evolution, 29(8):1969-1973.
[21]	EZARD T, FUJISAWA T, BARRACLOUGH T G, 2009. splits: SPecies’ LImits by Threshold Statistics. R package version 1.0-14/r31. http://R-Forge.R-project.org/ projects/splits/.
[22]	FOLMER O, BLACK M, HOEH W, et al, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol, 3(5):294-299. https://www.ncbi.nlm.nih.gov/pubmed/7881515 pmid: 7881515
[23]	FUJISAWA T, BARRACLOUGH T G, 2013. Delimiting species using single-locus data and the generalized mixed Yule coalescent approach: a revised method and evaluation on simulated data sets[J]. Systematic Biology, 62(5):707-724.
[24]	GELLER J, MEYER C, PARKER M, et al, 2013. Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all-taxa biotic surveys[J]. Molecular Ecology Resources, 13(5):851-861. https://www.ncbi.nlm.nih.gov/pubmed/23848937 pmid: 23848937
[25]	KING R A, READ D S, TRAUGOTT M, et al, 2008. INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA-based approaches[J]. Molecular Ecology, 17(4):947-963. https://www.ncbi.nlm.nih.gov/pubmed/18208490 pmid: 18208490
[26]	LERAY M, YANG J Y, MEYER C P, et al, 2013. A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents[J]. Frontiers in Zoology, 10(1):34.
[27]	MARKAIDA U, SOSA-NISHIZAKI O, 2003. Food and feeding habits of jumbo squid Dosidicus gigas (Cephalopoda: Ommastrephidae) from the Gulf of California, Mexico[J]. Journal of the Marine Biological Association of the United Kingdom, 83(3):507-522.
[28]	MEYER C P. 2003. Molecular systematics of cowries (Gastropoda: Cypraeidae) and diversification patterns in the tropics[J]. Biological Journal of the Linnean Society, 79(3):401-459.
[29]	PAGE R D M, 1996. TreeView: an application to display phylogenetic trees on personal computers[J]. Computer Applications in the Biosciences: CABIOS, 12(4):357-358. https://www.ncbi.nlm.nih.gov/pubmed/8902363 pmid: 8902363
[30]	POSADA D, CRANDALL K A, 2001. Selecting the best-fit model of nucleotide substitution[J]. Systematic Biology, 50(4):580-601. https://www.ncbi.nlm.nih.gov/pubmed/12116655 pmid: 12116655
[31]	SHCHETINNIKOV A S, 1992. Feeding spectrum of squid Sthenoteuthis oualaniensis (Oegopsida) in the eastern Pacific[J]. Journal of the Marine Biological Association of the United Kingdom, 72(4):849-860.
[32]	SYMONDSON W O C, 2002. Molecular identification of prey in predator diets[J]. Molecular Ecology, 11(4):627-641. https://www.ncbi.nlm.nih.gov/pubmed/11972753 pmid: 11972753
[33]	WARD R D, ZEMLAK T S, INNES B H, et al, 2005. DNA barcoding Australia's fish species[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462):1847-1857.
[34]	XU LEI, WANG LIANGGEN, NING JIAJIA, et al, 2019. Diversity of marine planktonic ostracods in South China Sea: a DNA taxonomy approach[J]. Mitochondrial DNA Part A, 30(1):118-125.

	站位	东经	北纬
鸢乌贼	S3	111°37'50"	14°58'04"
	S9	110°35'06"	14°02'10"
	S16	117°28'28"	13°00'00"
	S18	110°30'35"	11°02'09"
	S19	111°28'51"	10°59'30"
	S20	113°51'30"	11°02'17"
	S21	114°32'36"	11°06'13"
	S26	110°28'22"	9°30'53"
中国枪乌贼	SQ	118°29'21"	23°05'46"

引物名称	序列(5′-3′)	参考文献
HCO2198(下游)	TAAACTTCAGGGTGACCAAAAAATCA	Folmer et al, 1994
dgHCO2198(下游)	TAAACTTCAGGGTGACCAAARAAYCA	Meyer, 2003
jgHCO2198(下游)	TAIACYTCIGGRTGICCRAARAAYCA	Geller et al, 2013
mlCOIintF(上游)	GGWACWGGWTGAACWGTWTAYCCYCC	Leray et al, 2013

最佳匹配物种	相似度	序列号	样品号
克氏副葉鰺 Alepes Swainson	99.90%	KY371115.1	N0709、N0711、N0752、N0759、N0767、N0771、N0772
蓝圆鲹 Decapterus maruadsi	100.00%	MK264594.1	N0718
金带细鲹 Selaroides leptolepis	99.64%	JX261038.1	N0740、N0742、N0779
三线鯻 Terapon puta	100.00%	KY372209.1	N0722
怀氏方头鲳 Cubiceps whiteleggii	100.00%	KY371367.1	N0743
黄泽小沙丁鱼 Sardinella lemuru	100.00%	MK264430.1	N0758
角突仿对Kishinouyepenaeopsis amicus	98.71%	KR349255.1	N0720、N0723
赤虾属 Metapenaeopsis sp.	87.05%	KR150460.1	N0753
刺铠虾属 Munida sp.	86.58%	AY350938.1	N0749、N0754、N0757、N0761、N0768、N0769、N0775
招潮蟹属 Uca sp.	84.40%	AB813678.1	N0735
团扇蟹属 Ozius sp.	87.45%	HM638053.1	N0770
梭子蟹属Portunus sp.	88.67%	JX398096.1	N0777
中国枪乌贼 Uroteuthis chinensis	100.00%	MG192385.1	N0736

最佳匹配物种	相似度	序列号	样品号
孔锯鳞鱼 Myripristis kuntee	99.53%	MK658063.1	S20-41-2
尾斑棘鳞鱼 Sargocentron caudimaculatum	100.00%	MF409503.1	S19-44-4
克氏副葉鰺 Alepes Swainson	100.00%	MK264421.1	S3-23-3
脂眼凹肩鲹 Selar crumenophthalmus	100.00%	MH638727.1	S26-14-2
鲣鱼 Katsuwonus pelamis	99.78%	MH638766.1	S19-44-3、S16-24-3
金焰灯笼魚 Myctophum aurolaternatum	100.00%	KJ555427.1	S20-28-3
鸢乌贼微型群 Sthenoteuthis oualaniensis	100.00%	MF411130.1	S18-1、S18-14、S19-31-2
鸢乌贼 Sthenoteuthis oualaniensis	100.00%	MF411105.1	S21-45-1、S3-25、S9-22

Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 34

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XU Chao, LONG Lijuan, LI Sha, YUAN Li, XU Xiaolu. Systematic reorganization of historical data of scientific investigation in the South China Sea and its affiliated islands and reefs 3. data sharing service and application [J]. Journal of Tropical Oceanography, 2024, 43(5): 158-165.
[2]	XU Chao, LONG Lijuan, LI Sha, HE Yunkai, YUAN Li, XU Xiaolu. Systematic reorganization of historical data of scientific investigation in the South China Sea and its affiliated islands and reefs 1. data reorganization technology and application [J]. Journal of Tropical Oceanography, 2024, 43(5): 143-149.
[3]	XU Chao, LONG Lijuan, LI Sha, XU Xiaolu, YUAN Li. Systematic reorganization of historical data of scientific investigation in the South China Sea and its affiliated islands and reefs 2. data curation and application [J]. Journal of Tropical Oceanography, 2024, 43(5): 150-157.
[4]	LIU Yuan, KE Zhixin, LI Kaizhi, TAN Yehui, LIANG Junce, ZHOU Weihua. Zooplankton community in the coastal waters of eastern Guangdong under the influence of human activities and ocean currents [J]. Journal of Tropical Oceanography, 2024, 43(4): 98-111.
[5]	LIU Didi, ZHANG Xiyang, SUN Fulin, WANG Mingzhuang, TAN Fei, SHI Qi, WANG Guan, YANG Hongqiang. Microbial communities and specific strains within beachrocks of the South China Sea: implications for the origin of beachrock* [J]. Journal of Tropical Oceanography, 2024, 43(4): 112-122.
[6]	HU Simin, ZHOU Tiancheng, ZHANG Chen, LIU Sheng, LI Tao, HUANG Hui. Effect of suspended solids on zooplankton community and their feeding selectivity in the Sanya coral waters [J]. Journal of Tropical Oceanography, 2024, 43(3): 122-130.
[7]	JIANG Lyumiao, CHEN Tianran, ZHAO Kuan, ZHANG Ting, XU Lijia. Experimental study on bioerosion of marginal reefs in the Weizhou Island, northern South China Sea [J]. Journal of Tropical Oceanography, 2024, 43(3): 155-165.
[8]	JIA Nan, ZHOU Tiancheng, HU Simin, ZHANG Chen, HUANG Hui, LIU Sheng. Difference in the feeding contents of three hermit crabs in the coral reefs of the Nansha Islands, South China Sea [J]. Journal of Tropical Oceanography, 2024, 43(3): 109-121.
[9]	LIN Xianzhi, ZHOU Yanyan, LIN Haoye, HU Simin, HUANG Hui, ZHANG Li, LIU Sheng. Diet analysis of the parrotfish (Scarus globiceps) in coral reefs of the Nansha Islands [J]. Journal of Tropical Oceanography, 2024, 43(3): 100-108.
[10]	XU Lijia, LIAO Zhiheng, CHEN Hui, WANG Yongzhi, HUANG Baiqiang, LIN Qiaoyun, GAN Jianfeng, YANG Jing. Community structure of scleractinian corals in the northern South China Sea and their responses to the marine heatwaves [J]. Journal of Tropical Oceanography, 2024, 43(3): 58-71.
[11]	ZHAO Minghui, YUAN Ye, ZHANG Jiazheng, ZHANG Cuimei, GAO Jinwei, WANG Qiang, SUN Zhen, CHENG Jinhui. New developments on the rift-breakup of the continent-ocean transition zone in the northern margin of the South China Sea [J]. Journal of Tropical Oceanography, 2024, 43(2): 173-183.
[12]	HUANG Yu, WANG Lin, MAI Zhimao, LI Jie, ZHANG Si. Isolation and characterization of sand fixation ability of bacteria in biological soil crusts of the tropical islands, South China Sea [J]. Journal of Tropical Oceanography, 2023, 42(6): 101-110.
[13]	WANG Chenyan, SHI Jingwen, YAN Annan, KANG Yaru, WANG Yuxuan, QIN Suli, HAN Minwei, ZHANG Ruijie, YU Kefu. Bioaccumulation characteristics and source apportionment of organophosphate esters in Acanthaster planci from the South China Sea [J]. Journal of Tropical Oceanography, 2023, 42(5): 30-37.
[14]	LI Niu, DI Pengfei, FENG Dong, CHEN Duofu. The impact of cold seepage on geochemical indices for redox conditions of marine sediments ―Site F active seep site in the northeastern South China Sea^* [J]. Journal of Tropical Oceanography, 2023, 42(5): 144-153.
[15]	ZHANG Zhisheng, XIE Lingling, LI Junyi, LI Qiang. Comparative analysis of mesoscale eddy evolution during life cycle in marginal sea and open ocean: South China Sea and Kuroshio Extension [J]. Journal of Tropical Oceanography, 2023, 42(4): 63-76.