大亚湾核电站邻近水域桡足幼体现场摄食研究

doi:10.11978/2017058

Abstract

Abstract:

Copepods play important roles in maintaining the structure of marine ecosystem due to their key position in food chain and their numerous species, quantities and wide distribution. The nutrients obtained by copepod larvae can directly affect their development, then the complement of adult copepods and even their population stability. In this study, in situ feeding of copepodites was evaluated by molecular methods in the waters near the nuclear power plant in Daya Bay (S1: control station, S2: outfall station) in summer 2015. The results showed that 16 prey species in total were identified in both stations, including diatoms (11 species), tunicate (two species), fungi (one species), ichthyosporea (one species), and oomycetes (one species). Diatoms (47.30%) and tunicate (41.89%) were the most abundant food types revealed by the significant proportion of the clones. Similar feeding diversity was discovered in copepodites at S1 and S2, with nine prey species at S1 and 10 at S2, but significant differences in diet composition were revealed, with metazoan (61.54%) and diatoms (68.57%) dominating the diets of copepodites at S1 and S2, respectively. In addition, copepodites appeared to be more herbivorous at S2 with a lower Omnivory Index (0.31) than at S1 (0.72). These results suggested that copepodites could optimize their diet composition by consuming a reasonable percentage of animal and plant materials according to available food sources. More phytoplankton, especially diatoms as diet of copepodites at the outfall station implied that copepodites might change their feeding habits with global warming development.

Key words: Daya Bay, copepodites, feeding, diversity, increased temperature

Cuilian XU, Tao LI, Simin HU, Youjun WANG, Hui HUANG, Sheng LIU. In situ feeding of copepodites in the water near Daya Bay nuclear power plant[J].Journal of Tropical Oceanography, 2018, 37(2): 17-25.

Figures/Tables 5

Fig. 1

Tab. 1

Tab. 2

Fig. 2

Fig. 3

References 45

[1]	高亚辉, 李松, 1990. 瘦尾胸刺水蚤摄食率的观察实验[J]. 热带海洋, 9(3): 59-65.
	GAO YAHUI, LI SONG, 1990. Observational experiments on feeding rates of Centropages tenuiremis[J]. Tropic Oceanology, 9(3): 59-65 (in Chinese with English abstract).
[2]	金德祥, 陈金环, 黄凯歌, 1965.中国海洋浮游硅藻类[M]. 上海: 上海科学技术出版社: 58-209.
[3]	金鑫, 2011. 黄东海浮游食物网的初步研究-基于脂肪酸标记法和碳氮稳定同位素比值法[D]. 青岛: 中国科学院海洋研究所: 34-55.
	JIN XIN, 2011. Plankton food web analysis of the East China Sea and the Yellow Sea using analyses of fatty acids and stable isotopes[D]. Qingdao: The Institute of Oceanology, Chinese Academy of Sciences: 34-55 (in Chinese with English abstract).
[4]	李海涛, 杨官品, 石媛嫄, 等, 2006. 长菱形藻(Nitzschia longissima)和新月细柱藻(Cylindrotheca closterium)分子分类研究[J]. 海洋湖沼通报, (3): 67-72.
	LI HAITAO, YANG GUANPIN, SHI YUANYUAN, et al, 2006. Studies on molecular identification of Nitzschia longissima and Cylindrotheca closterium[J]. Transactions of Oceanology and Limnology, (3): 67-72 (in Chinese with English abstract).
[5]	李捷, 孙松, 李超伦, 等, 2006. 不同饵料对桡足类无节幼体存活、发育的影响研究[J]. 海洋科学, 30(12): 13-20.
	LI JIE, SUN SONG, LI CHAOLUN, et al, 2006. The effects of different diets on the survival and development of copepod nauplii[J]. Marine Sciences, 30(12): 13-20 (in Chinese with English abstract).
[6]	刘梦坛, 李超伦, 孙松, 2010. 两种甲藻和两种硅藻脂肪酸组成的比较研究[J]. 海洋科学, 34(10): 77-82.
	LIU MENGTAN, LI CHAOLUN, SUN SONG, 2010. Comparative study of fatty acid compositions of two dinoflagellates and two diatoms[J]. Marine Sciences, 34(10): 77-82 (in Chinese with English abstract).
[7]	孙军, 宋书群, 徐兆礼, 等, 2007. 东海米氏凯伦藻水华中中华哲水蚤的选择性摄食[J]. 海洋与湖沼, 38(6): 536-541.
	SUN JUN, SONG SHUQUN, XU ZHAOLI, et al, 2007. The selective grazing of Calanus sinicus during a Karenia mikimotoi bloom in the east China sea[J]. Oceanologia et Limnologia Sinica, 38(6): 536-541 (in Chinese with English abstract).
[8]	王小冬, 2006. 胶州湾浮游动物对浮游植物选择性摄食的初步研究[D]. 青岛: 中国海洋大学: 15-52.
	WANG XIAODONG, 2006. Preliminary studies on selective grazing of zooplankton on phytoplankton in Jiaozhou Bay[D]. Qingdao: Ocean University of China: 15-52 (in Chinese with English abstract).
[9]	杨纪明, 1998a. 渤海两种桡足类幼体摄食的初步研究[J]. 现代渔业信息, 13(6): 5-8.
	YANG JIMING, 1998a. Preliminary study on the feeding of copepodid larvae of two species in the Bohai Sea[J]. Modern Fisheries Information, 13(6): 5-8 (in Chinese with English abstract).
[10]	杨纪明, 1998b. 渤海中华哲水蚤桡足幼体摄食的初步研究[J]. 海洋水产研究, 19(2): 1-4.
	YANG JIMING, 1998b. Preliminary study on the feeding of copepodid larvae of the Bohai Sea Calanus sinicus brodsky[J]. Marine Fisheries Research, 19(2): 1-4 (in Chinese with English abstract).
[11]	杨庆霄, 1988. 温度对一些浮游植物中脂肪酸组成的影响[J]. 海洋与湖沼, 19(5): 439-446.
	YANG QINGXIAO, 1988. The effect of temperature on fatty acid composition of marine phytoplankton[J]. Oceanologia et Limnologia Sinica, 19(5): 439-446 (in Chinese with English abstract).
[12]	曾祥波, 黄邦钦, 陈纪新, 等, 2006. 台湾海峡小型浮游动物的摄食对夏季藻华演替的影响[J]. 海洋学报, 28(5): 107-116.
	ZENG XIANGBO, HUANG BANGQIN, CHEN JIXIN, et al, 2006. Grazing impact of microzooplankton on algal bloom in the Taiwan Strait in summer[J]. Acta Oceanologica Sinica, 28(5): 107-116 (in Chinese with English abstract).
[13]	张晓东, 2012. 厦门港骨条藻属Skeletonema (Bacillariophyta)物种多样性及周年变化的研究[D]. 青岛: 中国科学院海洋研究所: 36.
	ZHANG XIAODONG, 2012. Diversity and annual variation of Skeletonema (Bacillariophyta) in Xiamen Harbor waters[D]. Qingdao: The Institute of Oceanology, Chinese Academy of Sciences: 36 (in Chinese with English abstract).
[14]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会, 2008. GB/T 12763.6-2007 海洋调查规范第6部分: 海洋生物调查[S]. 北京: 中国标准出版社.
	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China, 2008. GB/T 12763.6-2007 Specifications for oceanographic survey—Part 6: Marine biological survey[S]. Beijing: China Standard Publishing House (in Chinese).
[15]	ADOLPH S, BACH S, BLONDEL M, et al, 2004. Cytotoxicity of diatom-derived oxylipins in organisms belonging to different phyla[J]. Journal of Experimental Biology, 207(17): 2935-2946.
[16]	BOERSMA M, MATHEW K A, NIEHOFF B, et al, 2016. Temperature driven changes in the diet preference of omnivorous copepods: no more meat when it's hot?[J]. Ecology Letters, 19(1): 45-53.
[17]	CALBET A, LANDRY M R, SCHEINBERG R D, 2000. Copepod grazing in a subtropical bay: species-specific responses to a midsummer increase in nanoplankton standing stock[J]. Marine Ecology Progress Series, 193: 75-84.
[18]	CHAO A, 1984. Nonparametric estimation of the number of classes in a population[J]. Scandinavian Journal of Statistics, 11(4): 265-270.
[19]	CORKETT C J, MCLAREN I A, 1970. Relationships between development rate of eggs and older stages of copepods[J]. Journal of the Marine Biological Association of the United Kingdom, 50(1): 161-168.
[20]	FENAUX R, 1985. Rhythm of secretion of oikopleurid's houses[J]. Bulletin of Marine Science, 37(2): 498-503.
[21]	FENCHEL T, 1988. Marine plankton food chains[J]. Annual Review of Ecology and Systematics, 19: 19-38.
[22]	GUO ZHILING, LIU SHENG, HU SIMIN, et al, 2012. Prevalent ciliate symbiosis on copepods: high genetic diversity and wide distribution detected using small subunit ribosomal RNA gene[J]. PLoS One, 7(9): e44847.
[23]	HEIDELBERG K B, SEBENS K P, PURCELL J E, 2004. Composition and sources of near reef zooplankton on a Jamaican forereef along with implications for coral feeding[J]. Coral Reefs, 23(2): 263-276.
[24]	HARRIS A S D, MEDLIN L K, LEWIS J, et al, 1995. Thalassiosira species (Bacillariophyceae) from a Scottish sea-loch[J]. European Journal of Phycology, 30: 117-131.
[25]	HU SIMIN, GUO ZHILIN, LI TAO, et al, 2014. Detecting in situ copepod diet diversity using molecular technique: development of a Copepod/Symbiotic Ciliate-Excluding Eukaryote-Inclusive PCR Protocol[J]. PLoS One, 9(7): e103528.
[26]	HU SIMN, GUO ZHILING, LI TAO, et al, 2015. Molecular analysis of in situ diets of coral reef copepods: evidence of terrestrial plant detritus as a food source in Sanya Bay, China[J]. Journal of Plankton Research, 37(2): 363-371.
[27]	IRIGOIEN X, HARRIS R P, VERHEYE H M, et al, 2002. Copepod hatching success in marine ecosystems with high diatom concentrations[J]. Nature, 419(6905): 387-389.
[28]	ISMAR S M, HANSEN T, SOMMER U, 2008. Effect of food concentration and type of diet on Acartia survival and naupliar development[J]. Marine Biology, 154(2): 335-343.
[29]	KRISHNAKUMAR V, SASTRY J S, SWAMY G N, 1991. Implication of thermal discharges into the sea-a review[J]. Indian Journal of Environmental Protection, 11(7): 525-527.
[30]	LAMBERT G, 2005. Ecology and natural history of the protochordates[J]. Canadian Journal of Zoology, 83(1): 34-50.
[31]	LARSEN P S, MADSEN C V, RIISGÅRD H U, 2008. Effect of temperature and viscosity on swimming velocity of the copepod Acartia tonsa, brine shrimp Artemia salina and rotifer Brachionus plicatilis[J]. Aquatic Biology, 4(1): 47-54.
[32]	LÓPEZ-URRUTIA Á, HARRIS R P, SMITH T, 2004. Predation by calanoid copepods on the appendicularian Oikopleura dioica[J]. Limnology and Oceanography, 49(1): 303-307.
[33]	LI TAO, LIU SHENG, HUANG LIANGMIN, et al, 2011. Diatom to dinoflagellate shift in the summer phytoplankton community in a bay impacted by nuclear power plant thermal effluent[J]. Marine Ecology Progress Series, 424: 75-85.
[34]	MEUNIER C L, BOERSMA M, WILTSHIRE K H, et al, 2016. Zooplankton eat what they need: copepod selective feeding and potential consequences for marine systems[J]. Oikos, 125(1): 50-58.
[35]	NAKAMURA Y, 1998. Blooms of tunicates Oikopleura spp. and Dolioletta gegenbauri in the Seto Inland Sea, Japan, during summer[J]. Hydrobiologia, 385(1-3): 183-192.
[36]	SHANNON C E, WEAVER W, 1949.The mathematical theory of communication[M]. Urbana, Champaign: University of Illinois Press: 6-28.
[37]	SIMPSON E H, 1949. Measurement of diversity[J]. Nature, 163(4148): 688.
[38]	SMITH J K, LONSDALE D J, GOBLER C J, et al, 2008. Feeding behavior and development of Acartia tonsa nauplii on the brown tide alga Aureococcus anophagefferens[J]. Journal of Plankton Research, 30(8): 937-950.
[39]	SWADLING K M, MARCUS N H, 1994. Selectivity in the natural diets of Acartia tonsa dana (Copepoda: Calanoida): Comparison of juveniles and adults[J]. Journal of Experimental Marine Biology and Ecology, 181(1): 91-103.
[40]	TAYLOR R L, ABRAHAMSSON K, GODHE A, et al, 2009. Seasonal variability in polyunsaturated aldehyde production potential among strains of Skeletonema marinoi (Bacillariophyceae)[J]. Journal of Phycology, 45(1): 46-53.
[41]	VAN DE WAAL D B, VERSCHOOR A M, VERSPAGEN J M H, et al, 2010. Climate-driven changes in the ecological stoichiometry of aquatic ecosystems[J]. Frontiers in Ecology and the Environment, 8(3): 145-152.
[42]	VIDOUDEZ C, POHNERT G, 2008. Growth phase-specific release of polyunsaturated aldehydes by the diatom Skeletonema marinoi[J]. Journal of Plankton Research, 30(11): 1305-1313.
[43]	YOUN S H, CHOI J K, 2007. Egg production of the copepod Acartia hongi in Kyeonggi Bay, Korea[J]. Journal of Marine Systems, 67(3-4): 217-224.
[44]	YU JING, TANG DANLING, YAO LIJUN, et al, 2010. Long-term water temperature variations in Daya Bay, China using satellite and in situ observations[J]. Terrestrial, Atmospheric and Oceanic Sciences, 21(2): 393-399.
[45]	ZHANG HUAN, LIN SENJIE, 2005. Development of a cob-18S rRNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment[J]. Applied and Environmental Microbiology, 71(11): 7053-7063.

	种类数		丰度百分比		细胞丰度/ (×10⁵cells·L^-1)	香农-威纳指数^a	优势种	叶绿素a含量/(μg·L^-1)
	甲藻	硅藻	甲藻	硅藻	细胞丰度/ (×10⁵cells·L^-1)	香农-威纳指数^a	优势种	叶绿素a含量/(μg·L^-1)
S1	7	14	66.14%	38.36%	4.38	1.89	Scrippsiella trochoidea	5.09
S2	5	12	71.94%	28.06%	3.92	1.39	Scrippsiella trochoidea	3.18

样品编号	种类数	有效克隆数	辛普森指数^b	香农-威纳指数^c	Chao1 指数^d
S1	9	39	0.6772	1.593	10.5
S2	10	35	0.8245	1.987	11

In situ feeding of copepodites in the water near Daya Bay nuclear power plant

RichHTML

PDF (PC)

Like

Knowledge

Cited

Abstract

Cite this article

share this article

Figures/Tables 5

References 45

Related Articles 15

Metrics

Comments

Recommended 0

[1]	RAO Yiyong, ZHAO Meirong, KUANG Zexing, HUANG Honghui, TAN Erhui. Influence of raft-string oyster culture on the functional structure of macrobenthic communities: a case study in the Dapeng Cove^* [J]. Journal of Tropical Oceanography, 2024, 43(5): 69-83.
[2]	LIU Weiwei, WEN Shaowei, TAN Yehui. Progress in studies on diversity and ecological role of ciliates in mariculture [J]. Journal of Tropical Oceanography, 2024, 43(4): 1-19.
[3]	MO Danyang, NING Zhiming, YANG Bin, XIA Ronglin, LIU Zhijin. Response of dissimilatory nitrate reduction processes in coral reef sediments of the Weizhou island to temperature changes [J]. Journal of Tropical Oceanography, 2024, 43(4): 137-143.
[4]	XI Chen, LIN Zongxuan, SA Rula, DENG Xi, LIU Qiang, NI Liang, LUO Laicai, MA Teng, XIE Zhijie, CHEN Siruo, CHEN Songze. Analysis of water environmental changes and influencing factors in the southwestern waters of the Daya Bay based on continuous monitoring data from dual buoys [J]. Journal of Tropical Oceanography, 2024, 43(4): 153-164.
[5]	HU Simin, ZHOU Tiancheng, ZHANG Chen, LIU Sheng, LI Tao, HUANG Hui. Effect of suspended solids on zooplankton community and their feeding selectivity in the Sanya coral waters [J]. Journal of Tropical Oceanography, 2024, 43(3): 122-130.
[6]	XU Lijia, LIAO Zhiheng, CHEN Hui, WANG Yongzhi, HUANG Baiqiang, LIN Qiaoyun, GAN Jianfeng, YANG Jing. Community structure of scleractinian corals in the northern South China Sea and their responses to the marine heatwaves [J]. Journal of Tropical Oceanography, 2024, 43(3): 58-71.
[7]	CHEN Junqiang, WANG Wenbo, WANG Qing, YANG Yufeng. Species diversity and habitat preference of bdelloid rotifers in the Weizhou island, Guangxi [J]. Journal of Tropical Oceanography, 2024, 43(2): 81-91.
[8]	HUANG Yu, WANG Lin, MAI Zhimao, LI Jie, ZHANG Si. Isolation and characterization of sand fixation ability of bacteria in biological soil crusts of the tropical islands, South China Sea [J]. Journal of Tropical Oceanography, 2023, 42(6): 101-110.
[9]	SUN Cuici, YUE Weizhong, ZHAO Wenjie, WANG Youshao. Distribution of the microbial Carbohydrate-Active enzymes genes in the surface sediment of the Daya Bay, China [J]. Journal of Tropical Oceanography, 2023, 42(5): 76-91.
[10]	SONG Xingyu, LIN Yajun, ZHANG Liangkui, XIANG Chenhui, HUANG Yadong, ZHENG Chuanyang. Distribution characteristics and influencing factors of meso- and micro-zooplankton communities in the offshore waters of the Guangdong-Hong Kong-Macao Greater Bay Area^* [J]. Journal of Tropical Oceanography, 2023, 42(3): 136-148.
[11]	CHENG Xiawen, ZHANG Lanlan, QIU Zhuoya, XIANG Rong, CHANG Hu. Biodiversity, biogeography and seasonal variation of zooplankton Collodarians (Radiolaria) in surface waters from the northern Indian Ocean to the South China Sea^* [J]. Journal of Tropical Oceanography, 2023, 42(2): 97-112.
[12]	WANG Zihan, ZENG Cong, JIANG Ziyu, CAO Ling. Conservation gap analysis of threatened fish in the East China Sea and adjacent sea areas [J]. Journal of Tropical Oceanography, 2023, 42(1): 66-86.
[13]	JIANG Xun, WU Wen, SONG Dehai. Identification and quantitative analysis of key controlling factors of water quality response to human activities in the Daya Bay, China [J]. Journal of Tropical Oceanography, 2023, 42(1): 182-191.
[14]	YE Jincheng, CHEN Yiqing, GAO Lin, ZHOU Xianjiao, ZHONG Cairong, ZHANG Ying, WANG Yun. Analysis of rhizosphere bacterial community characteristics of mangrove plant Sonneratia × gulngai and its parents [J]. Journal of Tropical Oceanography, 2022, 41(6): 75-89.
[15]	CHEN Jingfu, ZHONG Yu, WANG Lei, GUO Yupei, QIU Dajun. Noctiluca scintillans effects on eukaryotic plankton community structure using Environmental DNA analysis in Daya Bay* [J]. Journal of Tropical Oceanography, 2022, 41(5): 121-132.