基于形态学和高通量测序的春季南澳海域浮游植物群落特征及其与环境因子关系

doi:10.11978/2024046

Abstract

Abstract:

The structure of phytoplankton community was investigated based on morphological identification and high-throughput sequencing in the Nan’ao waters in May 2022. The spatial distribution of phytoplankton in relation to environmental factors was explored and results from two different methods were also compared and discussed. According to the cluster analysis of environmental factors, the survey area can be divided into nearshore area and offshore area. Totally, 105 species belong to 52 genera, 39 families, 27 orders, 8 orders, 5 phyla were morphological identified, while 543 species in 6 phyla, 32 orders, 97 families, 155 families, 272 genera through high-throughput sequencing were identified. Bacillariophyta and Dinophyta were the main groups, composing 74.54% and 24.78% through microscopic identification, and 17.52% and 67.84% through high-throughput sequencing, respectively. The abundance of phytoplankton identified by morphology ranged from 0.11×10⁵ to 6.85×10⁵cells·L^-1, which was significantly correlated with the distribution of chlorophyll a concentration, and the phytoplankton diversity index was lower than that of the high-throughput sequencing method. 7 dominant species were obtained by both methods, of which the absolutely dominant specie in the morphological identification results was Chaetoceros compressus, and the absolute dominant specie in the high-throughput sequencing identification results was Heterocapsa rotundata. Correlation analysis showed that the main environmental factor affecting phytoplankton community structure in Nan’ao in spring was pH, salinity and DIP (dissolved inorganic phosphorus). Using the combination of morphological identification and high-throughput sequencing technology, the structure and diversity of the phytoplankton community can be described more comprehensively and accurately.

Key words: Nan’ao Island, phytoplankton, community structure, high-throughput sequencing, environmental factor

CLC Number:

P735

ZHOU Zhixi, TANG Huijuan, KE Zhixin, LIU Jiaxing, ZHOU Weihua. Phytoplankton community structure and its relationship with environmental factors in the spring coastal region of Nan’ao based on morphology and high-throughput sequencing[J].Journal of Tropical Oceanography, 2025, 44(1): 53-65.

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因子	温度/℃	盐度/‰	pH	浊度/NTU	DIN/(μmol·L^-1)	DIP/(μmol·L^-1)	DSi/(μmol·L^-1)	氮磷比
近岸	23.55±0.39	28.04±1.85	8.03±0.09	4.17±2.63	12.31±4.39	0.77±0.59	24.56±4.55	26.13±21.53
远岸	23.22±0.50	31.37±0.78	8.16±0.06	1.93±2.22	2.06±0.64	0.16±0.09	7.20±3.61	105.08±18.47
t-test	t=1.528, P=0.187	t=5.126, P<0.01	t=5.665, P<0.01	t=1.53, P=0.187	t=5.81, P<0.05	t=2.95, P<0.05	t=8.93, P<0.01	t=17.45, P<0.01

分类水平		优势度		出现频率%
门	种	形态学鉴定	高通量测序	出现频率%
硅藻门Bacillariophyta	扁面角毛藻Chaetoceros compressus	0.33	-	83.33
	微眼藻Minutocellus sp.	-	0.05	100
	多形微眼藻Minutocellus polymorphus	-	0.03	100
	艾氏角毛藻Chaetoceros eibenii	0.02	-	58.33
	洛氏菱形藻Nitzschia lorenziana	0.02	-	83.33
	尖刺拟菱形藻Pseudo-nitzschia pungens	0.02	-	100
	中肋骨条藻Skeletonema costatum	0.02	-	41.67
甲藻门 Dinophyta	球形异帽藻Heterocapsa rotundata	-	0.22	100
	海洋原甲藻Prorocentrum micans	0.13	-	100
	剧毒卡洛藻Karlodinium veneficum	-	0.09	100
	单眼藻Warnowia sp.	-	0.09	100
	单眼藻Warnowia spp.		0.07	100
	锥状斯氏藻Scrippsiella trochoidea	0.02	-	83.33
绿藻门Chlorophyta	共球藻目Picochlorum sp.	-	0.03	100

Phytoplankton community structure and its relationship with environmental factors in the spring coastal region of Nan’ao based on morphology and high-throughput sequencing

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