水质变化对豆荚软珊瑚(Lobophytum sp.)共生藻(Symbiodiniaceae)、菌群落结构的影响

doi:10.11978/2024138

Abstract

Abstract:

In this study, Lobophytum sp. was cultured under different water qualities for one month, and the community structure of Symbiodiniaceae, ambient water bacteria, and symbiotic bacteria were analyzed using high-throughput sequencing technology. The findings indicated the following. 1) Symbiodiniaceae richness decreased significantly under nutrients conditions ranging from 0 to 80.64 μmol·L^-1nitrate and 0 to 1.05 μmol·L^-1phosphate. Cladocopium sp. dominated the Symbiodiniaceae in all three coral groups, with a relative abundance ranging from 70.25% to 98.13%, exhibiting higher tolerance to low nutrients concentration but greater sensitivity to high nutrients concentration. 2) At the phylum level, water-associated bacteria and coral symbiotic bacteria differed in relative abundance, with all dominant bacterial populations belonging to Proteobacteria, ranging from 45.63% to 86.55% in relative abundance. However, the environmental bacterial diversity at the genus level (Shannon index 4.60~4.97) was higher than that of coral symbiotic bacteria (Shannon index 2.58~3.81), with distinct taxonomic separation between the two communities at the genus level. 3) The coral symbiotic bacterium Cohaesibacter exhibited high tolerance to low nutrient levels, with its relative abundance increasing significantly from < 3% to 40.27% as nutrient levels decreased. Additionally, this genus of soft corals demonstrated strong adaptability to symbiotic bacteria Vibrio, with no significant anomalies observed even at a high Vibrio abundance of 23.71%. These results suggest that the abundance of Symbiodiniaceae and symbiotic bacteria in corals is influenced by ambient water quality, leading to changes in dominant bacteria and alterations in community structure of symbiotic bacteria from corals. Moreover, nutrient fluctuations preferentially shaped the community structure of coral-associated bacteria over environmental bacteria, with more pronounced effects on the former. This study contributes to the growing body of research on soft corals by providing a foundation for understanding how different water quality parameters dynamically affect the structure of symbiotic microorganisms in soft corals. It also offers insights into the effect of water quality fluctuations on soft coral Symbiodiniaceae and bacterial community structure in artificial environments, thereby supporting the development of coral conservation programs.

Key words: Lobophytum, Symbiodiniaceae, symbiotic bacteria, nutrients, community structure

CLC Number:

LI Da, WANG Yunzhong, QI Jiguang, YANG Cuihua. Effects of water quality changes on community structures of Symbiodiniaceae and symbiotic bacteria in soft coral Lobophytum sp.[J].Journal of Tropical Oceanography, 2025, 44(4): 136-144.

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珊瑚缸	红皮藻科	刺尾鱼科	雀鲷科	天竺鲷科	软珊瑚科	海葵科
1	Y	Y	N	N	Y	Y
2	N	N	Y	Y	N	Y
3	N	N	N	N	N	Y

珊瑚缸	水温/℃	盐度/‰	pH	亚硝态氮 /(μmol·L^-1)	氨氮 /(μmol·L^-1)	硝态氮 /(μmol·L^-1)	磷酸盐 /(μmol·L^-1)	KH/dKH	钙离子 /(μmol·L^-1)	镁离子 /(μmol·L^-1)
1	25	32	8.1~8.4	0~0.43	0~2.77	80.64~161.28	1.05~2.11	8~9	9.48~9.98	46.49~52.58
2	25	32	8.1~8.4	0~0.43	0~2.77	403.20~806.39	2.11~5.26	8~9	9.48~9.98	46.49~52.58
3	25	32	8.1~8.4	0~0.43	0~2.77	32.26~80.64	0~1.05	8~9	9.48~9.98	46.49~52.58

样本组名	效序列条数	平均长度/bp	最短序列长度/bp	最长序列长度/bp
C1	106234	289.89	240	369
C2	92647	305.81	201	369
C3	95787	283.77	237	385

样本组名	Cladocopium 相对丰度/%	Symbiodinium 相对丰度/%	未鉴定类相对丰度/%	其他类相对丰度/%
C1	90.69	1.01	8.18	0.12
C2	70.25	0.75	28.85	0.15
C3	98.13	1.14	0.13	0.60

样本组名	序列数	分类单元	Shannon 指数	Simpson 指数	Chao 指数	Ace 指数	覆盖率
C1	105818	20	0.42	0.82	21.00	22.82	1.00
C2	92363	22	0.74	0.56	22.00	22.00	1.00
C3	95698	14	0.15	0.95	14.00	14.00	1.00

Effects of water quality changes on community structures of Symbiodiniaceae and symbiotic bacteria in soft coral Lobophytum sp.

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样本组名	有效序列条数	平均长度/bp	最短序列长度/bp	最长序列长度/bp
W1	70993	410.70	351	468
W2	101847	410.76	350	463
W3	117782	409.87	350	456
B1	53373	422.88	354	462
B2	120092	416.80	350	465
B3	95859	406.93	350	457

样本组名	变形菌门	髌骨菌门	放线菌门	螺旋体门	蓝菌门	疣微菌门	拟杆菌门	蛭弧菌门	厚壁菌门	浮霉菌门
W1	53.46	6.21	17.33	0.01	1.52	3.62	5.68	2.17	1.15	2.26
W2	45.63	23.51	9.98	0.02	0.83	8.37	1.74	1.53	0.38	0.80
W3	55.98	19.64	4.21	0.05	1.55	5.47	2.22	2.96	0.14	1.16
B1	61.03	0.10	2.03	14.93	13.07	0.38	2.43	0.01	3.61	0.77
B2	69.75	0.07	3.24	14.56	3.52	0.64	4.73	0.26	1.90	0.31
B3	86.55	0.51	0.42	7.60	0.64	0.49	1.21	0.88	0.57	0.44

样本组名	序列数	分类单元	Shannon指数	Simpson指数	Chao指数	Ace指数	覆盖率
W1	53759	2746	4.60	0.13	2862.37	2924.59	0.99
W2	70846	2658	4.97	0.05	2793.00	2887.91	0.99
W3	112451	1809	4.89	0.06	1833.67	1822.23	1.00
B1	47324	608	3.33	0.09	647.65	656.31	1.00
B2	116473	1007	3.81	0.06	1066.35	1052.82	1.00
B3	88783	739	2.58	0.20	803.75	829.94	1.00

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